DDX54 (Homo sapiens) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  DDX54
Gene Name:  DEAD (Asp-Glu-Ala-Asp) box polypeptide 54
Original Clone ID:
FLH269965.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297197
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2643nts
Open reading frame: 1 to 2643

ATGGCGGCCGACAAGGGCCCGGCGGCTGGACCTCGGTCGCGAGCTGCCATGGCCCAGTGG
AGGAAGAAGAAAGGGCTCCGGAAGCGCCGAGGCGCGGCCTCCCAGGCCCGCGGCAGCGAC
TCGGAGGACGGCGAGTTTGAGATCCAGGCGGAAGATGACGCCCGGGCCCGGAAGCTGGGA
CCTGGAAGACCCCTGCCCACCTTCCCCACCTCGGAATGCACCTCGGATGTGGAGCCGGAC
ACCCGGGAGATGGTGCGTGCCCAGAACAAGAAGAAGAAGAAGTCTGGAGGCTTCCAGTCC
ATGGGCCTGAGCTACCCGGTGTTCAAAGGCATCATGAAGAAGGGGTACAAGGTGCCAACA
CCCATCCAGAGGAAGACCATCCCGGTGATCTTGGATGGCAAGGACGTGGTGGCCATGGCC
CGGACGGGCAGTGGCAAGACAGCCTGCTTCCTCCTCCCAATGTTCGAGCGGCTCAAGACC
CACAGTGCCCAGACCGGGGCCCGCGCCCTCATCCTCTCGCCGACCCGAGAGCTGGCCCTG
CAGACCCTGAAGTTCACTAAGGAGCTAGGCAAGTTCACTGGCCTCAAGACTGCCCTGATC
CTGGGTGGAGACAGGATGGAAGACCAGTTTGCAGCCCTGCACGAAAATCCCGACATAATT
ATTGCCACGCCCGGACGGTTGGTGCATGTGGCTGTGGAAATGAGCCTGAAGCTGCAGAGT
GTGGAATACGTGGTGTTCGATGAAGCTGACCGGCTTTTTGAAATGGGTTTCGCAGAGCAG
CTGCAGGAGATCATCGCCCGCCTCCCCGGGGGCCACCAGACGGTGCTGTTCTCCGCCACG
CTGCCCAAACTGCTGGTGGAATTTGCCCGGGCTGGCCTCACGGAGCCCGTGCTCATCCGG
CTTGACGTGGATACCAAGCTCAACGAGCAGCTGAAGACCTCCTTCTTCCTCGTGCGGGAG
GACACCAAGGCTGCCGTGCTGCTCCACCTGCTGCACAACGTGGTGCGGCCCCAGGACCAG
ACCGTGGTGTTTGTGGCCACGAAGCACCACGCCGAGTACCTCACTGAGCTGCTGACGACC
CAGCGGGTGAGCTGCGCCCACATCTACAGTGCCCTAGACCCGACAGCCCGCAAGATCAAT
CTCGCCAAATTCACGCTTGGCAAGTGCTCCACTCTCATTGTGACTGACCTGGCCGCCCGA
GGCCTGGACATCCCGCTGCTGGACAATGTCATCAACTACAGCTTCCCCGCCAAGGGCAAA
CTCTTCCTGCACCGCGTGGGCCGTGTGGCTCGGGCTGGCCGAAGTGGCACAGCCTACTCC
TTGGTGGCCCCTGATGAAATCCCCTACCTGCTGGATCTGCACCTGTTCCTGGGCCGCTCC
CTCACCCTCGCCCGACCCCTCAAGGAGCCCTCAGGTGTGGCCGGTGTGGATGGCATGCTG
GGTCGGGTGCCACAGAGTGTGGTGGACGAGGAGGACAGTGGTCTGCAGAGCACCCTGGAG
GCATCGCTGGAGCTACGGGGCCTGGCCCGCGTTGCTGATAACGCCCAGCAGCAGTATGTG
CGCTCACGCCCGGCGCCCTCGCCTGAGTCCATCAAGAGGGCCAAGGAGATGGACCTTGTG
GGGCTGGGCCTGCACCCCCTCTTCAGCTCGCGTTTTGAGGAGGAGGAGCTGCAGCGGCTG
AGGCTGGTGGACAGCATAAAGAACTACCGCTCCCGGGCGACTATCTTTGAGATCAACGCC
TCCAGCCGAGACCTGTGCAGCCAGGTGATGCGCGCCAAGCGGCAGAAGGACCGCAAGGCC
ATCGCCCGCTTCCAGCAGGGACAGCAGGGGCGGCAGGAGCAGCAGGAGGGCCCAGTGGGC
CCAGCCCCGAGCCGCCCAGCACTGCAGGAGAAGCAGCCTGAGAAGGAGGAGGAGGAGGAG
GCGGGAGAGAGTGTGGAGGACATTTTCTCAGAGGTCGTGGGCCGGAAGCGGCAGCGGTCA
GGACCCAACAGGGGAGCCAAGAGGCGGAGGGAGGAGGCCCGGCAGCGGGACCAGGAATTC
TACATCCCCTACCGGCCCAAGGACTTTGACAGCGAGCGGGGCCTGAGCATCAGCGGGGAA
GGGGGAGCCTTTGAGCAGCAGGCAGCTGGCGCTGTCCTGGACTTGATGGGGGATGAAGCC
CAGAACCTGACGAGGGGCCGGCAGCAGCTCAAGTGGGACCGTAAGAAGAAGCGGTTTGTG
GGACAGTCAGGACAGGAAGACAAGAAGAAGATTAAGACAGAGAGCGGCCGCTACATCAGC
AGCTCCTACAAGCGAGACCTCTATCAGAAGTGGAAACAGAAACAGAAAATTGATGATCGT
GACTCGGACGAAGAAGGGGCATCTGACCGGCGAGGCCCAGAGCGAAGAGGTGGGAAGCGA
GACCGTGGCCAAGGTGCATCCCGGCCCCACGCCCCAGGCACCCCTGCAGGCCGAGTCCGC
CCGGAACTCAAGACCAAGCAGCAGATCCTGAAGCAGCGGCGCCGGGCCCAGAAGCTGCAC
TTCCTGCAGCGTGGTGGCCTCAAGCAGCTCTCTGCCCGCAACCGCCGCCGCGTCCAGGAG
CTGCAGCAGGGCGCCTTCGGCCGGGGTGCCCGCTCCAAGAAGGGCAAGATGCGGAAGAGG
ATG

Protein sequence predicted based on nucleotide sequence

MAADKGPAAGPRSRAAMAQWRKKKGLRKRRGAASQARGSDSEDGEFEIQAEDDARARKLG
PGRPLPTFPTSECTSDVEPDTREMVRAQNKKKKKSGGFQSMGLSYPVFKGIMKKGYKVPT
PIQRKTIPVILDGKDVVAMARTGSGKTACFLLPMFERLKTHSAQTGARALILSPTRELAL
QTLKFTKELGKFTGLKTALILGGDRMEDQFAALHENPDIIIATPGRLVHVAVEMSLKLQS
VEYVVFDEADRLFEMGFAEQLQEIIARLPGGHQTVLFSATLPKLLVEFARAGLTEPVLIR
LDVDTKLNEQLKTSFFLVREDTKAAVLLHLLHNVVRPQDQTVVFVATKHHAEYLTELLTT
QRVSCAHIYSALDPTARKINLAKFTLGKCSTLIVTDLAARGLDIPLLDNVINYSFPAKGK
LFLHRVGRVARAGRSGTAYSLVAPDEIPYLLDLHLFLGRSLTLARPLKEPSGVAGVDGML
GRVPQSVVDEEDSGLQSTLEASLELRGLARVADNAQQQYVRSRPAPSPESIKRAKEMDLV
GLGLHPLFSSRFEEEELQRLRLVDSIKNYRSRATIFEINASSRDLCSQVMRAKRQKDRKA
IARFQQGQQGRQEQQEGPVGPAPSRPALQEKQPEKEEEEEAGESVEDIFSEVVGRKRQRS
GPNRGAKRRREEARQRDQEFYIPYRPKDFDSERGLSISGEGGAFEQQAAGAVLDLMGDEA
QNLTRGRQQLKWDRKKKRFVGQSGQEDKKKIKTESGRYISSSYKRDLYQKWKQKQKIDDR
DSDEEGASDRRGPERRGGKRDRGQGASRPHAPGTPAGRVRPELKTKQQILKQRRRAQKLH
FLQRGGLKQLSARNRRRVQELQQGAFGRGARSKKGKMRKRM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2674
Start on reference sequence 29
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable