Gene:
Gene Symbol:
COL15A1
Gene Name:
collagen, type XV, alpha 1
Original Clone ID:
FLH269972.01L
Keyword:
STOP codon removed based on OC annotation
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297204
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCACCAAGGAGGAACAACGGGCAGTGCTGGTGTCTGCTGATGCTGCTCTCGGTCTCC
ACGCCCCTCCCTGCTGTCACCCAGACCCGCGGTGCGACAGAGACTGCTTCCCAGGGTCAC
CTGGACCTCACGCAGCTCATCGGTGTCCCGCTGCCCTCGTCCGTATCCTTTGTCACAGGC
TATGGTGGCTTCCCGGCCTACAGTTTCGGGCCTGGTGCCAATGTTGGCCGCCCAGCCAGG
ACTCTCATCCCATCCACCTTCTTCAGGGACTTCGCCATCAGCGTCGTGGTGAAGCCCAGC
AGCACCCGTGGTGGCGTGCTCTTCGCCATCACTGACGCCTTCCAGAAGGTCATCTACCTG
GGCCTGCGGCTCTCAGGTGTGGAGGACGGCCACCAGCGGATCATCCTCTACTACACGGAG
CCAGGCTCCCATGTGTCCCAAGAGGCTGCTGCCTTCTCGGTGCCTGTGATGACCCACAGG
TGGAACCGCTTCGCCATGATTGTCCAGGGTGAGGAAGTGACCCTCCTCGTGAACTGTGAG
GAGCACAGCCGCATCCCCTTCCAGCGGTCCTCCCAGGCTTTGGCTTTTGAGTCCAGCGCT
GGAATCTTCATGGGCAATGCAGGAGCTACAGGGCTCGAGAGATTCACTGGCTCCCTCCAG
CAGCTCACCGTGCACCCCGACCCCAGGACTCCCGAGGAGCTGTGTGACCCTGAAGAGTCC
TCGGCATCTGGAGAGACCAGTGGGCTGCAGGAGGCAGACGGAGTAGCTGAGATCTTAGAA
GCCGTCACCTACACTCAAGCCTCGCCCAAAGAAGCAAAAGTTGAACCCATAAACACACCT
CCAACTCCATCCTCCCCCTTTGAAGACATGGAACTTTCTGGTGAACCTGTACCCGAGGGG
ACCCTGGAAACCACCAACATGAGCATCATCCAGCACAGCAGCCCCAAACAAGGGTCTGGT
GAGATCCTGAATGACACACTGGAGGGGGTTCATTCTGTGGATGGTGACCCCATTACTGAC
AGCGGCTCAGGGGCTGGGGCCTTCCTTGACATTGCTGAAGAAAAGAATTTAGCAGCAACA
GCAGCGGGGCTGGCCGAGGTGCCCATCAGCACTGCTGGAGAAGCAGAGGCCAGCAGTGTG
CCCACCGGGGGACCAACCCTCTCTATGTCCACGGAGAACCCAGAGGAAGGGGTCACTCCA
GGTCCAGATAATGAAGAGCGTTTAGCAGCAACAGCAGCAGGGGAGGCCGAGGCACTCGCC
AGCATGCCTGGGGAAGTGGAGGCCAGTGGTGTGGCCCCCGGGGAGCTGGACCTCTCCATG
TCCGCCCAGAGCCTCGGGGAAGAGGCCACTGTGGGTCCAAGCAGTGAAGACAGTTTAACA
ACAGCTGCAGCTGCAACCGAAGTGTCCCTCAGTACTTTTGAGGATGAGGAAGCCAGTGGG
GTCCCCACAGATGGCCTGGCTCCCCTCACAGCCACCATGGCCCCTGAGCGGGCAGTCACT
TCTGGTCCTGGTGATGAAGAAGACTTGGCAGCAGCCACAACAGAGGAGCCCCTCATCACA
GCTGGGGGTGAAGAGTCCGGCAGCCCTCCCCCTGATGGGCCACCGCTGCCCCTGCCCACA
GTGGCTCCTGAAAGATGGATCACTCCAGCTCAAAGAGAACATGTGGGAATGAAAGGACAG
GCTGGGCCCAAAGGAGAAAAGGGTGATGCTGGGGAGGAGCTTCCTGGCCCTCCTGAACCT
TCTGGGCCTGTTGGACCCACGGCAGGAGCAGAAGCAGAGGGCTCTGGCCTAGGCTGGGGC
TCGGACGTCGGCTCTGGCTCTGGTGACCTGGTGGGCAGTGAGCAGCTGCTGAGAGGTCCT
CCAGGACCCCCAGGGCCACCTGGCTTACCTGGGATTCCAGGAAAACCAGGAACTGATGTT
TTCATGGGACCCCCTGGATCTCCTGGAGAGGATGGACCTGCTGGTGAACCTGGGCCCCCG
GGCCCTGAGGGACAGCCTGGAGTTGATGGAGCCACCGGCCTTCCCGGGATGAAAGGGGAG
AAGGGAGCAAGAGGGCCTAATGGCTCAGTTGGTGAAAAGGGTGACCCTGGCAACAGAGGC
TTACCTGGACCCCCGGGGAAAAAGGGACAAGCTGGCCCTCCTGGGGTCATGGGACCCCCA
GGGCCTCCTGGACCCCCTGGGCCCCCAGGCCCTGGATGCACAATGGGACTTGGATTCGAG
GATACCGAAGGCTCTGGAAGCACCCAGCTATTGAATGAACCCAAACTCTCCAGACCAACG
GCTGCAATTGGTCTCAAAGGAGAGAAAGGAGACCGGGGACCCAAGGGAGAAAGGGGGATG
GATGGAGCCAGTATTGTGGGACCCCCTGGGCCGAGAGGGCCACCTGGGCACATCAAGGTC
TTGTCTAATTCCTTGATCAATATCACCCATGGATTCATGAATTTCTCGGACATTCCTGAG
CTGGTGGGGCCTCCGGGGCCGGACGGGTTGCCTGGGCTGCCAGGATTTCCAGGTCCTAGA
GGACCAAAAGGTGACACTGGTTTACCTGGCTTTCCAGGACTAAAAGGAGAACAGGGCGAG
AAGGGAGAGCCGGGTGCCATCCTGACAGAGGACATTCCTCTGGAAAGGCTGATGGGGAAA
AAGGGTGAACCTGGAATGCATGGAGCCCCAGGACCAATGGGGCCCAAAGGACCACCAGGA
CATAAAGGAGAATTTGGCCTTCCCGGGCGACCTGGTCGCCCAGGACTGAATGGCCTCAAG
GGTACCAAAGGAGATCCAGGGGTCATTATGCAGGGCCCACCTGGCTTACCTGGCCCTCCA
GGCCCCCCTGGGCCACCTGGAGCTGTGATTAACATCAAAGGAGCCATTTTCCCAATACCC
GTCCGACCACACTGCAAAATGCCAGTTGATACTGCTCATCCTGGGAGTCCAGAGCTCATC
ACTTTTCACGGTGTTAAAGGAGAGAAAGGATCCTGGGGTCTTCCTGGCTCAAAGGGAGAA
AAAGGCGACCAGGGAGCCCAGGGACCACCAGGTCCTCCACTTGATCTAGCTTACCTGAGA
CACTTTCTGAACAACTTGAAGGGGGAGAATGGAGACAAGGGGTTCAAAGGTGAAAAAGGA
GAAAAAGGAGACATTAATGGCAGCTTCCTTATGTCTGGGCCTCCAGGCCTGCCCGGAAAT
CCAGGCCCGGCTGGCCAAAAAGGGGAGACAGTCGTTGGGCCCCAAGGACCCCCAGGTGCT
CCTGGTCTGCCTGGGCCACCTGGCTTTGGAAGACCTGGTGATCCTGGGCCACCGGGGCCC
CCGGGGCCACCAGGACCTCCAGCTATCCTGGGAGCAGCTGTGGCCCTTCCAGGTCCCCCT
GGCCCTCCAGGACAGCCAGGGCTTCCCGGATCCAGAAACCTGGTCACAGCATTCAGCAAC
ATGGATGACATGCTGCAGAAAGCGCATTTGGTTATAGAAGGAACATTCATCTACCTGAGG
GACAGCACTGAGTTTTTCATTCGTGTTAGAGATGGCTGGAAAAAATTACAGCTGGGAGAA
CTGATCCCCATTCCTGCCGACAGCCCTCCACCCCCTGCGCTTTCCAGCAACCCACATCAG
CTTCTGCCTCCACCAAACCCTATTTCAAGTGCCAATTATGAGAAGCCTGCTCTGCATTTG
GCTGCTCTGAACATGCCATTTTCTGGGGACATTCGAGCTGATTTTCAGTGCTTCAAGCAG
GCCAGAGCTGCAGGACTGTTGTCCACCTACCGAGCATTCTTATCTTCCCATTTGCAAGAT
CTGTCCACCATTGTGAGGAAAGCAGAGAGATACAGCCTTCCCATAGTGAACCTCAAGGGC
CAAGTACTTTTTAATAATTGGGACTCAATTTTTTCTGGCCACGGAGGTCAGTTCAATATG
CATATTCCAATATACTCCTTTGATGGTCGAGACATAATGACAGATCCTTCTTGGCCCCAG
AAAGTCATTTGGCATGGCTCCAGCCCCCATGGCGTCCGCCTTGTGGATAACTACTGTGAA
GCATGGCGAACCGCGGACACAGCGGTCACGGGACTTGCCTCCCCGCTGAGCACGGGGAAG
ATTCTGGACCAGAAAGCATACAGCTGTGCTAATCGGCTAATTGTCCTATGTATCGAAAAC
AGTTTCATGACAGACGCTAGGAAG
Protein sequence predicted based on nucleotide sequence
MAPRRNNGQCWCLLMLLSVSTPLPAVTQTRGATETASQGHLDLTQLIGVPLPSSVSFVTG YGGFPAYSFGPGANVGRPARTLIPSTFFRDFAISVVVKPSSTRGGVLFAITDAFQKVIYL GLRLSGVEDGHQRIILYYTEPGSHVSQEAAAFSVPVMTHRWNRFAMIVQGEEVTLLVNCE EHSRIPFQRSSQALAFESSAGIFMGNAGATGLERFTGSLQQLTVHPDPRTPEELCDPEES SASGETSGLQEADGVAEILEAVTYTQASPKEAKVEPINTPPTPSSPFEDMELSGEPVPEG TLETTNMSIIQHSSPKQGSGEILNDTLEGVHSVDGDPITDSGSGAGAFLDIAEEKNLAAT AAGLAEVPISTAGEAEASSVPTGGPTLSMSTENPEEGVTPGPDNEERLAATAAGEAEALA SMPGEVEASGVAPGELDLSMSAQSLGEEATVGPSSEDSLTTAAAATEVSLSTFEDEEASG VPTDGLAPLTATMAPERAVTSGPGDEEDLAAATTEEPLITAGGEESGSPPPDGPPLPLPT VAPERWITPAQREHVGMKGQAGPKGEKGDAGEELPGPPEPSGPVGPTAGAEAEGSGLGWG SDVGSGSGDLVGSEQLLRGPPGPPGPPGLPGIPGKPGTDVFMGPPGSPGEDGPAGEPGPP GPEGQPGVDGATGLPGMKGEKGARGPNGSVGEKGDPGNRGLPGPPGKKGQAGPPGVMGPP GPPGPPGPPGPGCTMGLGFEDTEGSGSTQLLNEPKLSRPTAAIGLKGEKGDRGPKGERGM DGASIVGPPGPRGPPGHIKVLSNSLINITHGFMNFSDIPELVGPPGPDGLPGLPGFPGPR GPKGDTGLPGFPGLKGEQGEKGEPGAILTEDIPLERLMGKKGEPGMHGAPGPMGPKGPPG HKGEFGLPGRPGRPGLNGLKGTKGDPGVIMQGPPGLPGPPGPPGPPGAVINIKGAIFPIP VRPHCKMPVDTAHPGSPELITFHGVKGEKGSWGLPGSKGEKGDQGAQGPPGPPLDLAYLR HFLNNLKGENGDKGFKGEKGEKGDINGSFLMSGPPGLPGNPGPAGQKGETVVGPQGPPGA PGLPGPPGFGRPGDPGPPGPPGPPGPPAILGAAVALPGPPGPPGQPGLPGSRNLVTAFSN MDDMLQKAHLVIEGTFIYLRDSTEFFIRVRDGWKKLQLGELIPIPADSPPPPALSSNPHQ LLPPPNPISSANYEKPALHLAALNMPFSGDIRADFQCFKQARAAGLLSTYRAFLSSHLQD LSTIVRKAERYSLPIVNLKGQVLFNNWDSIFSGHGGQFNMHIPIYSFDGRDIMTDPSWPQ KVIWHGSSPHGVRLVDNYCEAWRTADTAVTGLASPLSTGKILDQKAYSCANRLIVLCIEN SFMTDARK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
207
End on reference sequence
4373
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
