Gene:
Gene Symbol:
CRNKL1
Gene Name:
crooked neck pre-mRNA splicing factor 1
Original Clone ID:
FLH269975.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297207
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGAC
AAAAACACATCCAGATTGGAGCTCCGTTCTTACTCCCTTGCAGGGCGCCACGGCTCGACA
GAGCCACTCGTCCTCGCCTGGTCATCCCAGTTCCGGAGGCTGACCTGGGGATGCGCCCTG
GACGCCCTGCACCGCAGTCCTTGCGTCGCTGCTAGTCAGCACGGCGTTACCCACCTCATC
CGCAGCTCCAGGACGCCGCACTCCACGCGCTGCCGCAAGGAGGACGCGCAGCCTGGTCAC
CATGGAAACGGTGCCGCCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTCAGTCCTGCAA
GTTCCGCTTCCGGTTCCGAGATCCTGTTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGC
TCTCAGAGCCGATTTCTAGCGTCCGTGCCGGGGACAGGTGTCCAGAGGTCGACTGCTGCA
GACATGGCGGCCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAA
AACAAAGCCCCGGCTGAGGTACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAA
AGAGAACTTGAGCTTCTTCCACCTCCACCTCAACAGAAGATCACAGATGAAGAAGAATTA
AATGATTATAAACTAAGGAAAAGGAAGACTTTTGAAGATAATATAAGAAAAAACAGGACT
GTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAAAGCCTAAAGGAGATTCAAAGG
GCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATTACACTCTGGCTG
AAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGAC
CGGGCCATAACAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAG
GAAATGTTGGGAAACGTTGCCGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAG
CCTGAGGAGCAAGCCTGGCACTCCTACATCAACTTTGAGCTGAGATACAAAGAGGTGGAT
CGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACCCTGATGTTAAGAACTGGATC
AAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAAAGTGTATGAG
AGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCC
AAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTG
GACAGAATTTCAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAG
AAGTTTGGTGATAGGCGGGGTATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTAC
GAAGAAGAAGTGAAGGCGAATCCACACAATTATGATGCATGGTTTGATTACTTGCGCTTG
GTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCTATGAAAGGGCCATTGCCAAT
GTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTGGATCAACTAT
GCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCC
TCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCA
CAGTTTGAAATACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATA
GGCAAATGTCCAAAGAACAAATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGA
GAATTTGACAGATGCCGGAAGCTTTATGAAAAGTTCCTGGAATTTGGACCTGAAAATTGT
ACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTGGTGATATTGACAGAGCACGG
GCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGTGCTTTGGAAA
TCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGG
CGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTG
TCTTCAGGAAAAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAA
ACCATGCGAAACTGTGAAGAAAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGT
TTTGAAGAAGAATTTGGAACAGCTTCAGATAAGGAGAGAGTAGACAAACTCATGCCAGAG
AAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGTCTGATGCAGGCTGGGAAGAA
TACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAAACTCCTGGCC
ATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGAT
GAGGACGTCGATGAGAGTGAATCC
Protein sequence predicted based on nucleotide sequence
MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCAL DALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQ VPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVK NKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRT VISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWD RAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVD RARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQY EEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINY ALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSI GKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRAR AIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFEL SSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPE KVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD EDVDESES
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2579
Start on reference sequence
33
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
