Gene:
Gene Symbol:
LTBP4
Gene Name:
latent transforming growth factor beta binding protein 4
Original Clone ID:
FLH269979.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297211
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGGAGACGTAAAAGCGTTGCTGTTTGTCGCTGCTGCCCGGGCCAGACGTCTAGGAGGA
GCCGCTGCATCCGAGTCCCTGGCTGTCTCCGAAGCCTTCTGCAGGGTCCGAAGCTGCCAG
CCCAAAAAGTGTGCAGGCCCCCAGCGGTGCCTGAACCCAGTGCCTGCAGTGCCCAGTCCC
AGCCCCAGCGTGAGGAAGAGACAGGTGTCCCTCAACTGGCAGCCACTGACGCTCCAGGAG
GCCAGAGCTCTACTGAAGCGGCGGCGGCCCCGGGGGCCAGGGGGCCGGGGACTACTGAGA
AGGAGGCCCCCACAGCGTGCCCCCGCTGGCAAGGCCCCGGTCCTGTGTCCCTTGATCTGT
CACAATGGCGGTGTGTGCGTGAAGCCTGACCGCTGCCTCTGTCCCCCGGACTTCGCTGGC
AAGTTCTGCCAGTTGCACTCCTCGGGCGCCCGGCCCCCGGCCCCGGCTGTACCAGGCCTC
ACCCGCTCCGTGTACACTATGCCACTGGCCAACCACCGCGACGACGAGCACGGCGTGGCA
TCTATGGTGAGCGTCCACGTGGAGCACCCGCAGGAGGCGTCGGTGGTGGTGCACCAGGTG
GAGCGTGTGTCTGGCCCTTGGGAGGAGGCGGACGCTGAGGCGGTGGCGCGGGCGGAAGCG
GCGGCGCGGGCGGAGGCGGCAGCGCCCTACACGGTGTTGGCACAGAGCGCGCCGCGGGAG
GACGGCTACTCAGATGCCTCGGGCTTCGGTTACTGCTTTCGGGAGCTGCGCGGAGGCGAA
TGCGCGTCCCCGCTGCCCGGGCTCCGGACGCAGGAGGTCTGCTGCCGAGGGGCCGGCTTG
GCCTGGGGCGTTCACGACTGTCAGCTGTGCTCCGAGCGCCTGGGGAACTCCGAAAGAGTG
AGCGCCCCAGATGGACCTTGTCCAACCGGCTTTGAAAGAGTTAATGGGTCCTGCGAAGAT
GTGGATGAGTGCGCGACTGGCGGGCGCTGCCAGCACGGCGAGTGTGCAAACACGCGCGGC
GGGTACACGTGTGTGTGCCCCGACGGCTTTCTGCTCGACTCGTCCCGCAGCAGCTGCATC
TCCCAACACGTGATCTCAGAGGCCAAAGGGCCCTGCTTCCGCGTGCTCCGCGACGGCGGC
TGTTCGCTGCCCATTCTGCGGAACATCACTAAACAGATCTGCTGCTGCAGCCGCGTAGGC
AAGGCCTGGGGCCGGGGCTGCCAGCTCTGCCCACCCTTCGGCTCAGAGGGTTTCCGGGAG
ATCTGCCCGGCTGGTCCTGGTTACCACTACTCGGCCTCCGACCTCCGCTACAACACCAGA
CCCCTGGGCCAGGAGCCACCCCGAGTGTCACTCAGCCAGCCTCGTACCCTGCCAGCCACC
TCTCGGCCATCTGCAGGCTTTCTGCCCACCCATCGCCTGGAGCCCCGGCCTGAACCCCGG
CCCGATCCCCGGCCCGGCCCTGAGCTTCCCTTGCCCAGCATCCCTGCCTGGACTGGTCCT
GAGATTCCTGAATCAGGTCCCTCCTCCGGCATGTGTCAGCGCAACCCCCAGGTCTGCGGC
CCAGGACGCTGCATTTCCCGGCCCAGCGGCTACACCTGCGCTTGCGACTCTGGCTTCCGG
CTCAGCCCCCAGGGCACCCGATGCATTGATGTGGACGAATGTCGCCGCGTGCCCCCGCCC
TGTGCTCCCGGGCGCTGCGAGAACTCACCAGGCAGCTTCCGCTGCGTGTGCGGCCCGGGC
TTCCGAGCCGGCCCACGGGCTGCGGAATGCCTGGATGTGGACGAGTGCCACCGCGTGCCG
CCGCCGTGTGACCTCGGGCGCTGCGAGAACACGCCAGGCAGCTTCCTGTGCGTGTGCCCC
GCCGGGTACCAGGCTGCACCGCACGGAGCCAGCTGCCAGGATGTGGATGAATGCACCCAG
AGCCCAGGCCTGTGTGGCCGAGGGGCCTGCAAGAACCTGCCTGGCTCTTTCCGCTGTGTT
TGCCCGGCTGGCTTCCGGGGCTCGGCGTGTGAAGAGGATGTGGATGAGTGTGCCCAGGAG
CCGCCGCCCTGTGGGCCCGGCCGCTGTGACAACACGGCAGGCTCCTTTCACTGTGCCTGC
CCTGCTGGCTTCCGCTCCCGAGGGCCCGGGGCCCCCTGCCAAGATGTGGATGAGTGTGCC
CGAAGCCCCCCACCCTGCACCTACGGCCGGTGTGAGAACACAGAAGGCAGCTTCCAGTGT
GTCTGCCCCATGGGCTTCCAACCCAACACTGCTGGCTCCGAGTGCGAGGATGTGGATGAG
TGTGAGAACCACCTCGCATGCCCTGGGCAGGAGTGTGTGAACTCGCCCGGCTCCTTCCAG
TGCAGGACCTGTCCTTCTGGCCACCACCTGCACCGTGGCAGATGCACTGATGTGGACGAA
TGCAGTTCGGGTGCCCCTCCCTGTGGTCCCCACGGCCACTGCACTAACACCGAAGGCTCC
TTCCGCTGCAGCTGCGCGCCAGGCTACCGGGCGCCGTCGGGTCGGCCCGGGCCCTGCGCA
GACGTGAACGAGTGCCTGGAGGGCGATTTCTGCTTCCCTCACGGCGAGTGCCTCAACACT
GACGGCTCCTTTGCCTGTACTTGTGCCCCTGGCTACCGACCCGGACCCCGCGGAGCCTCT
TGCCTCGACGTTGACGAGTGCAGCGAGGAGGACCTTTGCCAGAGCGGCATCTGTACCAAC
ACCGACGGCTCCTTCGAGTGCATCTGTCCTCCGGGACACCGCGCTGGCCCGGACCTCGCC
TCCTGCCTCGACGTGGACGAATGTCGCGAGCGAGGCCCAGCCCTGTGCGGGTCGCAGCGC
TGTGAGAACTCTCCCGGCTCCTACCGCTGTGTCCGGGACTGCGATCCTGGGTACCACGCG
GGCCCCGAGGGCACCTGTGACGATGTGGATGAGTGCCAAGAATATGGTCCCGAGATTTGT
GGAGCCCAGCGTTGTGAGAACACCCCTGGCTCCTACCGCTGCACACCAGCCTGTGACCCT
GGCTATCAGCCCACGCCAGGGGGCGGATGCCAGGATGTGGACGAATGCCGGAACCGGTCC
TTCTGCGGTGCCCACGCCGTGTGCCAGAACCTGCCCGGCTCCTTCCAGTGCCTCTGTGAC
CAGGGTTACGAGGGGGCACGGGATGGGCGTCACTGCGTGGATGTGAACGAGTGTGAAACA
CTACAGGGTGTATGTGGAGCTGCCCTGTGTGAAAATGTCGAAGGCTCCTTCCTCTGTGTC
TGCCCCAACAGCCCGGAAGAGTTTGACCCCATGACTGGACGCTGTGTTCCCCCACGAACT
TCTGCTGGCACGTTCCCAGGCTCGCAGCCCCAGGCACCTGCTAGCCCCGTTCTGCCCGCC
AGGCCACCTCCGCCACCCCTGCCCCGCCGACCCAGCACACCTAGGCAGGGCCCTGTGGGG
AGTGGGCGCCGGGAGTGCTACTTTGACACAGCGGCCCCGGATGCATGTGACAACATCCTG
GCTCGGAATGTGACATGGCAGGAGTGCTGCTGTACTGTGGGTGAGGGCTGGGGCAGCGGC
TGCCGCATCCAGCAGTGCCCGGGCACCGAGACAGCTGAGTACCAGTCATTGTGCCCTCAC
GGCCGGGGCTACCTGGCGCCCAGTGGAGACCTGAGCCTCCGGAGAGACGTGGACGAATGT
CAGCTCTTCCGAGACCAGGTGTGCAAGAGTGGCGTGTGTGTGAACACGGCCCCGGGCTAC
TCATGCTATTGCAGCAACGGCTACTACTACCACACACAGCGGCTGGAGTGCATCGACAAT
GACGAGTGCGCCGATGAGGAACCGGCCTGTGAGGGCGGCCGCTGTGTCAACACTGTGGGC
TCTTATCACTGTACCTGCGAGCCCCCACTGGTGCTGGATGGCTCGCAGCGCCGCTGCGTC
TCCAACGAGAGCCAGAGCCTCGATGACAATCTGGGAGTGTGCTGGCAGGAAGTGGGGGCT
GACCTCGTGTGCAGCCACCCTCGGCTGGACCGTCAGGCCACCTACACAGAGTGCTGCTGC
CTGTATGGAGAGGCCTGGGGCATGGACTGCGCCCTCTGCCCTGCGCAGGACTCAGATGAC
TTCGAGGCCCTGTGCAATGTGCTACGCCCCCCCGCATATAGCCCCCCGCGACCAGGTGGC
TTTGGACTCCCCTACGAGTACGGCCCAGACTTAGGTCCACCTTACCAGGGCCTCCCATAT
GGGCCTGAGTTGTACCCACCACCTGCGCTACCCTACGACCCCTACCCACCGCCACCTGGG
CCCTTCGCCCGCCGGGAGGCTCCTTATGGGGCACCCCGCTTCGACATGCCAGACTTTGAG
GACGATGGTGGCCCCTATGGCGAATCTGAGGCTCCTGCGCCACCTGGCCCGGGCACCCGC
TGGCCCTATCGGTCCCGGGACACCCGCCGCTCCTTCCCAGAGCCCGAGGAGCCTCCTGAA
GGTGGAAGCTATGCTGGTTCCCTGGCTGAGCCCTACGAGGAGCTGGAGGCGGAGGAGTGC
GGGATCCTGGACGGCTGCACCAACGGCCGCTGCGTGCGCGTCCCCGAAGGCTTCACCTGC
CGTTGCTTCGACGGCTACCGCCTGGACATGACCCGCATGGCCTGCGTTGACATCAACGAG
TGTGATGAGGCCGAGGCTGCCTCCCCGCTGTGCGTCAACGCGCGTTGCCTCAACACGGAT
GGCTCCTTCCGCTGCATCTGCCGCCCGGGATTCGCACCCACGCACCAGCCGCACCACTGT
GCGCCCGCACGGCCCCGGGCC
Protein sequence predicted based on nucleotide sequence
MGDVKALLFVAAARARRLGGAAASESLAVSEAFCRVRSCQPKKCAGPQRCLNPVPAVPSP SPSVRKRQVSLNWQPLTLQEARALLKRRRPRGPGGRGLLRRRPPQRAPAGKAPVLCPLIC HNGGVCVKPDRCLCPPDFAGKFCQLHSSGARPPAPAVPGLTRSVYTMPLANHRDDEHGVA SMVSVHVEHPQEASVVVHQVERVSGPWEEADAEAVARAEAAARAEAAAPYTVLAQSAPRE DGYSDASGFGYCFRELRGGECASPLPGLRTQEVCCRGAGLAWGVHDCQLCSERLGNSERV SAPDGPCPTGFERVNGSCEDVDECATGGRCQHGECANTRGGYTCVCPDGFLLDSSRSSCI SQHVISEAKGPCFRVLRDGGCSLPILRNITKQICCCSRVGKAWGRGCQLCPPFGSEGFRE ICPAGPGYHYSASDLRYNTRPLGQEPPRVSLSQPRTLPATSRPSAGFLPTHRLEPRPEPR PDPRPGPELPLPSIPAWTGPEIPESGPSSGMCQRNPQVCGPGRCISRPSGYTCACDSGFR LSPQGTRCIDVDECRRVPPPCAPGRCENSPGSFRCVCGPGFRAGPRAAECLDVDECHRVP PPCDLGRCENTPGSFLCVCPAGYQAAPHGASCQDVDECTQSPGLCGRGACKNLPGSFRCV CPAGFRGSACEEDVDECAQEPPPCGPGRCDNTAGSFHCACPAGFRSRGPGAPCQDVDECA RSPPPCTYGRCENTEGSFQCVCPMGFQPNTAGSECEDVDECENHLACPGQECVNSPGSFQ CRTCPSGHHLHRGRCTDVDECSSGAPPCGPHGHCTNTEGSFRCSCAPGYRAPSGRPGPCA DVNECLEGDFCFPHGECLNTDGSFACTCAPGYRPGPRGASCLDVDECSEEDLCQSGICTN TDGSFECICPPGHRAGPDLASCLDVDECRERGPALCGSQRCENSPGSYRCVRDCDPGYHA GPEGTCDDVDECQEYGPEICGAQRCENTPGSYRCTPACDPGYQPTPGGGCQDVDECRNRS FCGAHAVCQNLPGSFQCLCDQGYEGARDGRHCVDVNECETLQGVCGAALCENVEGSFLCV CPNSPEEFDPMTGRCVPPRTSAGTFPGSQPQAPASPVLPARPPPPPLPRRPSTPRQGPVG SGRRECYFDTAAPDACDNILARNVTWQECCCTVGEGWGSGCRIQQCPGTETAEYQSLCPH GRGYLAPSGDLSLRRDVDECQLFRDQVCKSGVCVNTAPGYSCYCSNGYYYHTQRLECIDN DECADEEPACEGGRCVNTVGSYHCTCEPPLVLDGSQRRCVSNESQSLDDNLGVCWQEVGA DLVCSHPRLDRQATYTECCCLYGEAWGMDCALCPAQDSDDFEALCNVLRPPAYSPPRPGG FGLPYEYGPDLGPPYQGLPYGPELYPPPALPYDPYPPPPGPFARREAPYGAPRFDMPDFE DDGGPYGESEAPAPPGPGTRWPYRSRDTRRSFPEPEEPPEGGSYAGSLAEPYEELEAEEC GILDGCTNGRCVRVPEGFTCRCFDGYRLDMTRMACVDINECDEAEAASPLCVNARCLNTD GSFRCICRPGFAPTHQPHHCAPARPRA
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4764
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
