Gene:
Gene Symbol:
Lgr5
Gene Name:
leucine rich repeat containing G protein coupled receptor 5
Original Clone ID:
FLH270082.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297219
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2721nts
Open reading frame: 1 to 2721
ATGGACACCTCCTGCGTCCACATGCTCCTGTCCTTGCTGGCGCTGCTGCAGTTGGTGGCC
GCCGGCAGCTCACCGGGACCAGATGCGATACCGCGGGGCTGCCCATCACACTGTCACTGT
GAGCTGGATGGCAGGATGCTGCTCAGGGTGGACTGCTCGGACCTGGGGCTCTCGGAGCTG
CCCTCCAACCTCAGCGTCTTCACCTCCTACCTGGACCTCAGTATGAACAACATCAGTCAG
CTACCCGCCAGTCTCCTACATCGCCTCTGCTTCCTAGAAGAGTTACGTCTTGCTGGAAAT
GCTTTGACACACATTCCCAAGGGAGCGTTCACGGGCCTTCACAGCCTCAAAGTGCTTATG
CTGCAGAACAACCAGCTGAGACAGGTTCCGGAGGAAGCGCTACAGAATTTGAGAAGCCTT
CAATCCCTGCGCCTAGATGCCAACCACATCAGCTACGTGCCACCCAGCTGTTTCAGCGGC
CTGCACTCCCTGAGGCACCTGTGGCTAGATGACAATGCTCTCACAGACGTCCCTGTCCAG
GCTTTCAGAAGTTTATCAGCCCTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCAC
ATAGCAGACTACGCCTTTGGAAACCTCTCCAGCCTCGTGGTTCTGCATCTCCATAATAAT
AGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGACTCCACAGCCTGGAGACTTTAGAT
TTAAATTATAATAACCTTGATGAATTCCCCACTGCAATCAAGACACTCTCCAACCTTAAG
GAACTAGGATTCCACAGCAACAACATCAGGTCAATACCGGAGCGAGCGTTCGTAGGCAAC
CCTTCTCTTATCACAATACACTTCTATGACAACCCCATCCAATTTGTTGGAGTATCTGCT
TTTCAGCATTTGCCTGAACTAAGAACACTGACTTTGAATGGTGCCTCGCACATTACTGAA
TTTCCTCACTTGACAGGAACTGCCACCCTGGAGAGTCTGACTTTAACTGGAGCAAAGATC
TCATCTCTTCCCCAGGCCGTCTGTGATCAGTTACCTAATCTCCAAGTGCTAGATTTGTCT
TACAACCTACTCGAAGACTTACCCAGTTTGTCAGGCTGCCAAAAACTTCAGAAAATTGAC
CTGAGGCATAACGAGATCTATGAAATTAAGGGCAGCACTTTTCAGCAGTTGTTTAACCTC
CGATCTCTGAACTTAGCATGGAATAAAATTGCTATCATTCACCCCAATGCGTTTTCTACG
TTGCCGTCTCTAATAAAGTTGGACCTATCATCCAATCTCCTGTCGTCCTTCCCTGTGACT
GGGTTACATGGTTTAACTCACTTAAAATTAACAGGGAACCGAGCCTTACAGAGCCTGATA
CCATCTGCAAACTTCCCAGAGCTCAAGATTATAGAAATGCCATCTGCTTACCAGTGTTGT
GCATTTGGGGGGTGTGAGAATGTCTATAAAATTTCTAACCAATGGAATAAAGACGACGGC
AACAGTGTGGACGACCTTCATAAGAAAGACGCTGGGTTATTTCAAGTTCAAGATGAGCGG
GACCTTGAAGATTTCCTACTTGACTTTGAGGAAGACCTGAAGGCCCTTCACTCGGTGCAG
TGCTCGCCTTCCCCAGGTCCCTTCAAGCCCTGTGAGCACCTATTTGGTAGCTGGCTGATC
CGAATCGGGGTGTGGACCACGGCAGTACTGGCGCTTTCCTGCAATGCCTTGGTGGCTTTG
ACCGTGTTCAGAACTCCTCTGTACATCTCTTCCATAAAGCTGCTAATTGGGGTAATCGCG
GTAGTGGACATTCTCATGGGGGTCTCCAGTGCTGTGCTGGCTGCCGTGGATGCATTCACT
TTTGGCCGTTTTGCTCAGCACGGTGCGTGGTGGGAAGACGGAATCGGCTGCCAAATCGTT
GGCTTCCTGTCCATTTTTGCTTCCGAATCGTCGATCTTCCTGCTCACTCTGGCAGCGCTG
GAACGAGGTTTTTCTGTCAAGTGCTCTTCGAAGTTTGAAGTGAAAGCTCCCCTTTTTAGC
CTGAGAGCGATCGTTTTGCTATGTGTCCTGTTGGCCCTGACCATTGCCACAATCCCCTTG
CTAGGAGGCAGTAAGTACAATGCCTCTCCCCTCTGCCTGCCCTTGCCCTTTGGGGAGCCC
AGCACCACGGGCTACATGGTGGCTCTCGTGTTGCTCAACTCTCTCTGTTTCCTCATAATG
ACCATTGCCTACACAAAGCTCTACTGCAGTTTGGAGAAAGGAGAGCTGGAGAATCTTTGG
GATTGTTCGATGGTGAAGCACATTGCTCTGTTGCTCTTCGCCAACTGCATCCTTTACTGC
CCCGTGGCTTTCTTATCCTTCTCCTCTTTGCTAAACCTCACCTTTATCAGTCCTGACGTC
ATTAAATTTATACTTCTCGTGATCGTCCCACTTCCTTCCTGTCTCAACCCACTTCTCTAC
ATTGTCTTCAATCCCCATTTTAAGGAGGATATGGGCAGCCTGGGAAAGCATACCCGTTTC
TGGATGAGATCAAAACACGCGAGTCTGCTGTCCATTAACTCGGACGATGTTGAGAAACGG
TCCTGTGAGTCAACCCAAGCCTTAGTATCCTTTACCCACGCCAGCATAGCCTATGACTTG
CCTTCCACTTCCGGGGCATCACCAGCTTACCCCATGACTGAAAGCTGTCATCTCTCTTCA
GTTGCATTTGTCCCATGTCTC
Protein sequence predicted based on nucleotide sequence
MDTSCVHMLLSLLALLQLVAAGSSPGPDAIPRGCPSHCHCELDGRMLLRVDCSDLGLSEL PSNLSVFTSYLDLSMNNISQLPASLLHRLCFLEELRLAGNALTHIPKGAFTGLHSLKVLM LQNNQLRQVPEEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTDVPVQ AFRSLSALQAMTLALNKIHHIADYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLD LNYNNLDEFPTAIKTLSNLKELGFHSNNIRSIPERAFVGNPSLITIHFYDNPIQFVGVSA FQHLPELRTLTLNGASHITEFPHLTGTATLESLTLTGAKISSLPQAVCDQLPNLQVLDLS YNLLEDLPSLSGCQKLQKIDLRHNEIYEIKGSTFQQLFNLRSLNLAWNKIAIIHPNAFST LPSLIKLDLSSNLLSSFPVTGLHGLTHLKLTGNRALQSLIPSANFPELKIIEMPSAYQCC AFGGCENVYKISNQWNKDDGNSVDDLHKKDAGLFQVQDERDLEDFLLDFEEDLKALHSVQ CSPSPGPFKPCEHLFGSWLIRIGVWTTAVLALSCNALVALTVFRTPLYISSIKLLIGVIA VVDILMGVSSAVLAAVDAFTFGRFAQHGAWWEDGIGCQIVGFLSIFASESSIFLLTLAAL ERGFSVKCSSKFEVKAPLFSLRAIVLLCVLLALTIATIPLLGGSKYNASPLCLPLPFGEP STTGYMVALVLLNSLCFLIMTIAYTKLYCSLEKGELENLWDCSMVKHIALLLFANCILYC PVAFLSFSSLLNLTFISPDVIKFILLVIVPLPSCLNPLLYIVFNPHFKEDMGSLGKHTRF WMRSKHASLLSINSDDVEKRSCESTQALVSFTHASIAYDLPSTSGASPAYPMTESCHLSS VAFVPCL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
288
End on reference sequence
3011
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
