Gene:
Gene Symbol:
Adcy6
Gene Name:
adenylate cyclase 6
Original Clone ID:
FLH270093.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297230
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3504nts
Open reading frame: 1 to 3504
ATGTCATGGTTTAGTGGCCTCCTGGTTCCCAAAGTGGATGAACGGAAAACAGCTTGGGGG
GAACGCAATGGGCAGAAGCGCCCACGCCACGCGAATCGAGCCAGTGGCTTCTGCGCACCT
CGCTACATGAGCTGCCTCAAGAATGCGGAGCCACCCAGCCCCACTCCTGCAGCTCACACT
CGGTGCCCCTGGCAGGATGAAGCCTTCATCAGGAGGGCGGGCCCGGGCAGGGGTGTGGAG
CTGGGGCTGCGGTCAGTGGCCTTGGGGTTTGACGACACTGAGGTGACCACACCGATGGGC
ACAGCTGAAGTGGCACCGGATACATCGCCTCGGAGCGGTCCGTCCTGCTGGCACCGGCTT
GTGCAGGTGTTCCAGTCTAAGCAGTTCCGCTCTGCCAAGCTGGAGCGGCTGTACCAGCGG
TACTTCTTCCAGATGAACCAGAGCAGCCTCACGCTGCTCATGGCGGTGCTGGTGCTGCTC
ATGGCTGTACTGTTGACTTTCCACGCTGCGCCTGCCCAGCCTCAGCCTGCTTACGTGGCC
CTGCTGACCTGTGCCTCTGTCCTTTTTGTGGTACTCATGGTGGTGTGTAACCGACACAGC
TTCCGCCAGGACTCCATGTGGGTGGTGAGCTATGTGGTCCTGGGCATCCTAGCAGCCGTG
CAAGTCGGGGGTGCCCTGGCAGCCAATCCACACAGCCCCTCGGCGGGCCTTTGGTGCCCC
GTGTTCTTCGTCTACATCACCTACACTCTTCTTCCCATTCGCATGCGAGCCGCAGTACTC
AGCGGCCTGGGCCTCTCTACTCTGCATTTGATTTTGGCCTGGCAGCTCAACAGCAGCGAC
CCCTTCCTTTGGAAGCAGCTCGGTGCTAACGTGGTGCTCTTCCTCTGCACCAATGCCATC
GGTGTCTGCACACACTACCCTGCTGAAGTGTCTCAGCGCCAAGCCTTTCAGGAGACCCGA
GGTTACATCCAGGCGCGGCTGCACCTGCAGCATGAGAACCGTCAGCAGGAACGGCTGCTG
CTATCGGTGTTGCCCCAGCACGTTGCCATGGAGATGAAAGAAGACATCAACACAAAAAAA
GAGGACATGATGTTCCATAAGATCTACATCCAGAAGCATGATAATGTCAGCATCCTGTTT
GCGGACATTGAGGGCTTCACCAGCCTGGCCTCCCAGTGCACTGCACAGGAACTGGTCATG
ACCTTGAATGAGCTCTTTGCCCGGTTTGACAAGCTGGCTGCGGTGAGGGAGAATCACTGT
CTGAGGATCAAGATCTTAGGAGACTGTTACTACTGCGTGTCAGGGCTGCCCGAGGCCCGG
GCAGATCACGCCCACTGCTGTGTGGAGATGGGGGTAGACATGATCGAAGCCATCTCGCTG
GTGCGTGAGGTAACAGGTGTGAACGTGAACATGCGTGTGGGCATCCACAGCGGACGTGTG
CATTGCGGCGTCCTTGGCCTACGGAAATGGCAGTTTGATGTCTGGTCAAACGATGTGACC
CTGGCTAACCACATGGAGGCCGGGGGCCGGGCCGGCCGCATCCACATCACTCGGGCTACA
CTGCAGTACTTGAACGGGGACTATGAGGTGGAGCCAGGCCGTGGTGGTGAACGCAATGCG
TACCTCAAGGAGCAGTGCATTGAGACCTTCCTCATACTTGGCGCCAGCCAAAAACGGAAA
GAGGAGAAAGCCATGCTGGCCAAGCTTCAGCGGACACGGGCCAACTCCATGGAAGGACTG
ATGCCCCGCTGGGTTCCTGACCGTGCCTTCTCCCGGACCAAGGACTCTAAGGCATTCCGC
CAGATGGGCATTGATGATTCTAGCAAAGACAACCGGGGTGCCCAAGATGCTCTGAACCCT
GAAGATGAGGTGGATGAGTTCCTGGGCCGAGCCATCGATGCCCGAAGCATCGACCAACTG
CGTAAGGACCATGTGCGCCGGTTCCTGCTCACCTTCCAGAGAGAGGATCTTGAGAAGAAG
TATTCACGGAAAGTAGATCCTCGCTTCGGAGCCTACGTCGCCTGTGCCCTCCTGGTTTTT
TGCTTCATCTGTTTTATCCAGCTCCTTGTGTTCCCATACTCCACCCTGATACTCGGGATT
TATGCCGCTATCTTCCTGCTGTTGCTGGTCACTGTGCTGATCTGTGCCGTGTGCTCCTGC
GGTTCTTTCTTCCCCAAGGCCCTGCAACGCCTGTCCCGCAATATTGTCCGCTCACGGGTG
CACAGCACCGCGGTTGGAATCTTCTCGGTTCTGCTTGTGTTCATCTCTGCCATCGCCAAC
ATGTTTACCTGTAATCACACCCCAATAAGGACCTGCGCGGCCCGGATGCTGAACTTAACA
CCAGCGGATGTCACCGCCTGCCACCTACAACAGCTCAATTACTCTCTGGGACTGGATGCT
CCCCTGTGTGAGGGCACCGCACCCACCTGCAGCTTCCCTGAGTACTTCGTCGGGAACGTG
CTGCTGAGTCTTCTAGCCAGCTCTGTCTTCCTACACATCAGCAGCATCGGCAAGCTGGCC
ATGACCTTCATCTTGGGGTTCACCTACTTGGTGCTGCTTTTGCTGGGTCCCCCGGCCGCC
ATCTTTGACAACTATGATCTACTGCTTGGCGTCCATGGCTTGGCTTCCTCCAATGAGACC
TTTGATGGGCTGGACTGCCCAGCTGTGGGGAGGGTAGCGCTCAAATATATGACCCCCGTG
ATTCTGCTGGTGTTTGCCCTGGCACTGTATCTGCATGCACAACAGGTGGAATCGACTGCC
CGCCTGGACTTCCTGTGGAAGTTACAGGCAACAGGGGAGAAGGAGGAGATGGAGGAGCTA
CAGGCATACAACCGGAGGTTGCTGCATAACATTCTTCCCAAGGACGTGGCCGCCCACTTC
CTGGCCCGGGAACGCCGCAACGATGAGCTGTACTACCAGTCGTGTGAATGTGTGGCTGTC
ATGTTTGCCTCCATCGCCAATTTCTCGGAGTTCTACGTGGAGCTCGAGGCAAACAACGAG
GGCGTGGAGTGCCTGCGGCTGCTCAATGAGATCATCGCAGACTTTGACGAGATCATCAGT
GAGGAGAGATTCCGGCAGTTGGAGAAGATCAAGACCATCGGTAGCACCTACATGGCCGCC
TCTGGGCTAAATGCCAGCACCTATGACCAGGTCGGCCGCTCACACATCACGGCGCTGGCT
GACTATGCCATGCGGCTCATGGAGCAGATGAAACACATCAATGAACACTCTTTCAACAAT
TTCCAGATGAAGATCGGGTTGAACATGGGTCCGGTTGTAGCAGGCGTCATTGGGGCCCGA
AAGCCACAGTATGACATCTGGGGAAATACCGTGAATGTTTCCAGTCGTATGGACAGCACT
GGAGTTCCTGACCGAATACAGGTGACTACGGACCTATACCAGGTTCTAGCTGCCAAGGGC
TACCAGCTGGAGTGTCGAGGGGTGGTCAAGGTGAAGGGAAAGGGGGAGATGACCACCTAC
TTCCTCAACGGGGGCCCCAGCAGT
Protein sequence predicted based on nucleotide sequence
MSWFSGLLVPKVDERKTAWGERNGQKRPRHANRASGFCAPRYMSCLKNAEPPSPTPAAHT RCPWQDEAFIRRAGPGRGVELGLRSVALGFDDTEVTTPMGTAEVAPDTSPRSGPSCWHRL VQVFQSKQFRSAKLERLYQRYFFQMNQSSLTLLMAVLVLLMAVLLTFHAAPAQPQPAYVA LLTCASVLFVVLMVVCNRHSFRQDSMWVVSYVVLGILAAVQVGGALAANPHSPSAGLWCP VFFVYITYTLLPIRMRAAVLSGLGLSTLHLILAWQLNSSDPFLWKQLGANVVLFLCTNAI GVCTHYPAEVSQRQAFQETRGYIQARLHLQHENRQQERLLLSVLPQHVAMEMKEDINTKK EDMMFHKIYIQKHDNVSILFADIEGFTSLASQCTAQELVMTLNELFARFDKLAAVRENHC LRIKILGDCYYCVSGLPEARADHAHCCVEMGVDMIEAISLVREVTGVNVNMRVGIHSGRV HCGVLGLRKWQFDVWSNDVTLANHMEAGGRAGRIHITRATLQYLNGDYEVEPGRGGERNA YLKEQCIETFLILGASQKRKEEKAMLAKLQRTRANSMEGLMPRWVPDRAFSRTKDSKAFR QMGIDDSSKDNRGAQDALNPEDEVDEFLGRAIDARSIDQLRKDHVRRFLLTFQREDLEKK YSRKVDPRFGAYVACALLVFCFICFIQLLVFPYSTLILGIYAAIFLLLLVTVLICAVCSC GSFFPKALQRLSRNIVRSRVHSTAVGIFSVLLVFISAIANMFTCNHTPIRTCAARMLNLT PADVTACHLQQLNYSLGLDAPLCEGTAPTCSFPEYFVGNVLLSLLASSVFLHISSIGKLA MTFILGFTYLVLLLLGPPAAIFDNYDLLLGVHGLASSNETFDGLDCPAVGRVALKYMTPV ILLVFALALYLHAQQVESTARLDFLWKLQATGEKEEMEELQAYNRRLLHNILPKDVAAHF LARERRNDELYYQSCECVAVMFASIANFSEFYVELEANNEGVECLRLLNEIIADFDEIIS EERFRQLEKIKTIGSTYMAASGLNASTYDQVGRSHITALADYAMRLMEQMKHINEHSFNN FQMKIGLNMGPVVAGVIGARKPQYDIWGNTVNVSSRMDSTGVPDRIQVTTDLYQVLAAKG YQLECRGVVKVKGKGEMTTYFLNGGPSS
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
155
End on reference sequence
3661
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
