Gene:
Gene Symbol:
Arhgef6
Gene Name:
Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6
Original Clone ID:
FLH270120.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297257
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2313nts
Open reading frame: 1 to 2313
ATGAATCCAGAAGAACGCCTTGTGACATGGCTTATATCTTTGGGAGTTCTAGAGTCTCCC
AAAAAGACTGTCTGTGATCCAGAGGAGTTCTTGAAGTCCTCGCTGAAAAATGGGGTAGTG
TTGTGCAAACTCATAAACAGACTTCTGCCTGGCTCGGTGGAGAAGTATTGTCTGGAACCC
CAAACTGAGGCCGACTGCATCGACAACATCAATGACTTCCTGAAAGGATGTGCAACTCTT
CAAGTGGAAGTGTTTGAGCCTGATGACCTCTATTCGGGGGCCAATTTCTCCAAGGTTCTG
AATACTCTTTTAGCTGTCAACAAAGCAACTGAAGATCAGCTCTCAGAAAGACCATGTGGA
AGGTCCTCTTCTCTTAGTGCTGCGACCAGCTCTCAGACAAACCCGCAGGTGGCCGTTCCC
AGCACAGCTCCAGAGCAGCACAGCGAGGAAAAGGCAGAGATGACGGAAAATGGAAGTCAC
CAGTTGATAGTAAAGGCAAGATTCAACTTCAAGCAGACGAATGAAGATGAATTGTCAGTG
TGTAAAGGGGACATCATTTATGTCACACGAGTTGAAGAAGGAGGCTGGTGGGAAGGCACA
TTAAATGGGAGAACTGGATGGTTCCCTAGTAATTATGTCCGAGAAATTAAACCCAGTGAG
AGACCTCTGTCCCCTAAGGCTATCAAAGGATTTGACACTGCTCCACTTACCAAGAATTAC
TACACTGTGGTGTTACAGAACATCTTGGACACTGAAAAAGAATATGCCAAAGAACTTCAG
TCTCTTCTTGTTACTTACCTAAGACCCTTGCAGTCTAATAACAACCTGAGTACTGTGGAG
TTTACATGTTTATTGGGAAACTTTGAAGAAGTATGCACATTTCAACAGACACTCTGCCAA
GCCTTGGAAGAATGTTCAAAGTTTCCAGAAAACCAACACAAAGTAGGAGGCTGCCTACTG
AACCTCATGCCTCATTTCAAATCGATGTACCTGGCTTACTGTGCAAATCATCCATCAGCT
GTCAACGTGCTCACCCAGCACAGTGATGATCTAGAACGATTCATGGAAAATCAAGGTGCA
TCCAGCCCGGGTATCCTCATTCTAACAACAAGTCTCAGCAAACCATTCATGCGACTGGAA
AAGTATGTTACTCTCTTGCAAGAACTGGAACGACATATGGAGGACACTCATCCAGACCAT
CAGGATATTCTGAAAGCAATCATAGCATTCAAAACACTCATGGGGCAGTGTCAAGATCTG
AGAAAGAGAAAGCAGCTGGAATTGCAGATCCTTTCAGAACCTATTCAGGCATGGGAAGGA
GATGATATTAAGACTTTGGGAAATGTGATCTTTATGTCTCAAGTAGTGATGCAGCATGGG
GCCTGTGAGGAGAAAGAGGAACGATATTTTCTGCTGTTTTCAAGTGTCTTGATAATGTTG
TCTGCAAGTCCTCGGATGAGTGGCTTTATGTATCAGGGAAAAATACCAATAGCAGGGATG
GTAGTGAACAGATTGGATGAAATTGAAGGCAGTGACTGTATGTTCGAAATCACAGGTAGC
ACGGTTGAGAGAATTGTGGTCCATTGCAACAATAACCAGGACTTCCAGGAGTGGATGGAG
CAGCTGAACAGACTGACCAAAGGACCAACCTCTTGCGGTTCATTGTCTAAGACCTCCTCC
TCATCATGTAGTACTCATTCTTCTTTTAGCTCTACCGGACAGCCCCGAGGACCCTTGGAG
CCTCCTCAAATTATAAAACCGTGGAGTTTAAGTTGCCTACGACCTGCACCTCCACTTAGA
CCATCAGCAGCACTAGGTTATAAAGAGAGGATGTCTTATATCTTAAAGGAATCTAGTAAG
AGCCCCAAAACAATGAAGAAGTTTCTTCACAAAAGAAAGACGGAGAGGAAAGCATCCGAG
GAAGAATACGTGATTAGGAAAAGTACTGCTGCCCTGGAAGAGGATGCTCAGATTCTTAAG
GTGATCGAAGCTTATTGCACCAGCGCCAGTTTTCAACAAGGTACTCGTAAAGATTCTGTT
CCACAAGTTCTACTCCCCGAGGAAGAGAAACTCATTATTGAAGAAACCAGAAGTAATGGG
CAGACCATCATTGAAGAGAAGAGCCTTGTTGATACTGTCTATGCCTTGAAGGATGAGGTC
AAGGAACTGAAGCAGGAGAATAAAAAAATGAAGCAATGTCTGGAAGAAGAATTGAAGTCG
AGGAAGGACCTAGAGAAGCTGGTCCGGAAGCTGTTGAAACAAACAGATGAATGCATTCGA
TCTGAATCCAGCAGCAAGACCTCGATTCTCCAG
Protein sequence predicted based on nucleotide sequence
MNPEERLVTWLISLGVLESPKKTVCDPEEFLKSSLKNGVVLCKLINRLLPGSVEKYCLEP QTEADCIDNINDFLKGCATLQVEVFEPDDLYSGANFSKVLNTLLAVNKATEDQLSERPCG RSSSLSAATSSQTNPQVAVPSTAPEQHSEEKAEMTENGSHQLIVKARFNFKQTNEDELSV CKGDIIYVTRVEEGGWWEGTLNGRTGWFPSNYVREIKPSERPLSPKAIKGFDTAPLTKNY YTVVLQNILDTEKEYAKELQSLLVTYLRPLQSNNNLSTVEFTCLLGNFEEVCTFQQTLCQ ALEECSKFPENQHKVGGCLLNLMPHFKSMYLAYCANHPSAVNVLTQHSDDLERFMENQGA SSPGILILTTSLSKPFMRLEKYVTLLQELERHMEDTHPDHQDILKAIIAFKTLMGQCQDL RKRKQLELQILSEPIQAWEGDDIKTLGNVIFMSQVVMQHGACEEKEERYFLLFSSVLIML SASPRMSGFMYQGKIPIAGMVVNRLDEIEGSDCMFEITGSTVERIVVHCNNNQDFQEWME QLNRLTKGPTSCGSLSKTSSSSCSTHSSFSSTGQPRGPLEPPQIIKPWSLSCLRPAPPLR PSAALGYKERMSYILKESSKSPKTMKKFLHKRKTERKASEEEYVIRKSTAALEEDAQILK VIEAYCTSASFQQGTRKDSVPQVLLPEEEKLIIEETRSNGQTIIEEKSLVDTVYALKDEV KELKQENKKMKQCLEEELKSRKDLEKLVRKLLKQTDECIRSESSSKTSILQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
228
End on reference sequence
2543
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
