Dgki (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Dgki
Gene Name:  diacylglycerol kinase, iota
Original Clone ID:
FLH270131.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297268
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3213nts
Open reading frame: 1 to 3213

ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCC
CGCGCTCCCGCCGCCTCCAGCGCCCTCAGCCCGACCGGCCTCTGCAGCGGCACCACCTCG
GCTTCTTTCGCGGCCGCCGGAGCGGTCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAG
AGAGGGGCGACGGGTGGCAGCAGCAGCAGCGGAAGCGGCGCCGGGAGCTGCTGCCTGGGC
GCCGAGGGCGGCGCGGACCCGCGGGGTGCAGGGGCAGCGGCTGCTGCTGCCTTGGAGGAG
CCCGCGGCCGCCGGACAGAAAGAGAAGGAAGAGGCGCTGGAGGAGAAGCTGAGGGACTTA
ACTTTCCGGAAGCAGGTCTCTTACAGGAAAGCCATCTCCCGGACAGGCCTCCAGCATCTG
GCACCTGCACATCCTCTTGGTCTTCCTGTGGCTAACGGTCCAGCGAAGGAGCCCAGAGCC
ACTCTGGACTGGAGCGAGAATGCTGTGAATGGGGAGCACCTGTGGCTGGAGACCAACGTT
TCAGGGGACCTCTGCTACCTGGGGGAGGAGAACTGCCAAGTCCGATTTGCAAAATCCGCT
CTCAGGAGGAAGTGTGCCGTCTGCAAAATCGTGGTCCACACAGCCTGCATTGAGCAACTA
GAGAAGATTAATTTCAGATGTAAACCAACATTTCGGGAAGGAGGCTCCAGATCGCCAAGA
GAAAACTTTGTGCGTCACCACTGGGTCCACAGGAGGCGGCAGGAGGGAAAATGTAAGCAG
TGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAGATCGTGGCCATCAGC
TGTTCTTGGTGCAAGCAGGCGTTTCACAATAAAGTGACCTGCTTCATGCTGCATCATATT
GAGGAACCCTGCTCCCTGGGGGCTCACGCCGCTGTTATTGTCCCGCCCACCTGGATCATT
AAGGTGAAGAAACCTCAGAACTCCCTGAAGGCTTCCAACCGGAAGAAGAAGAGGACAAGT
TTTAAAAGGAAAGCCAGTAAAAGAGGAACTGAGCAGGAAACCAAAGGCCGTCCTTTTGTG
ATCAAGCCCATCTCCTCCCCTCTCATGAAGCCTTTGCTTGTCTTTGTGAACCCAAAGAGT
GGGGGAAACCAGGGAACCAAAGTCCTACAGATGTTTATGTGGTACCTGAATCCACGGCAA
GTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTGGAGATGTACAGGAAGGTACCA
AATCTGAGAATTCTGGCCTGCGGTGGGGATGGAACGGTGGGCTGGATCCTCTCCATCCTG
GACGAGCTGCAGCTGAGCCCACAGCCTCCTGTGGGGGTGCTTCCACTGGGGACTGGGAAT
GACCTGGCTCGGACGCTCAACTGGGGAGGGGGTTACACCGATGAGCCTGTTTCTAAGATC
CTGTGCCAGGTGGAAGATGGGACCATTGTACAGTTGGATCGCTGGAACCTCCACGTAGAG
AGAAACCCAGACCTGCCGCCCGAAGAACTTGAAGATGGCGTGTGTAAGCTTCCTCTGAAT
GTCTTCAATAATTACTTCAGCCTTGGATTTGATGCTCACGTCACATTGGAGTTCCATGAA
TCCCGAGAAGCAAATCCAGAGAAATTCAATAGTCGTTTTCGAAATAAAATGTTCTATGCA
GGGGCAGCCTTTTCTGATTTCCTACAAAGAAGTTCTAGAGATCTATCCAAACACGTCAAA
GTTGTCTGTGATGGGACAGACCTCACCCCAAAGATCCAGGACCTGAAGTTCCAGTGTATA
GTATTTTTAAATATTCCCAGATATTGTGCTGGCACAATGCCTTGGGGAAACCCTGGAGAT
CATCATGACTTTGAACCCCAACGTCATGATGACGGCTACATTGAAGTCATTGGATTCACT
ATGGCCTCTTTGGCTGCCCTGCAAGTTGGGGGGCACGGAGAAAGGCTGCACCAGTGTCGG
GAAGTGATGCTTCTGACTTACAAGTCCATTCCCATGCAAGTGGACGGGGAGCCATGTCGG
TTGGCACCTGCTATGATTCGGATCTCCTTGAGGAATCAGGCCAACATGGTACAGAAGAGC
AAGCGGAGAACCTCGATGCCTTTGCTCAATGACATCCATCAGGTGCAAGCTGCGGACCTG
CGGCGAGTGTCTGCTCCCCCCGGCTCCTTCACCATTCCCCAGTCTGTCCCAGATCGCCTG
AGGATCCGTGTGAACAAAATCAGTTTACAAGACTATGAAGGACTCCACTATGACAAGGAC
AAACTCCGGGAAGCTTCCATCCCTCTGGGTATTCTAGTTGTTCGTGGAGACTGTGATTTG
GAGACTTGCCGTATGTACATAGACCGTCTACAGGAGGACTTGCAGTCAGTTTCTTCTGGG
TCACAGAGAGTTCATTACCAGGACCAAGAAACCTCCTTCCCCAGGGCACTCTCAGCTCAG
AGGCTGTCCCCACGGTGGTGCTTCCTAGATGCAACGTCTGCTGACCGCTTTTATCGAATA
GACAGATCTCAGGAACATTTGCACTTTGTGATGGAGATTTCTCACGATGAAATTTTTATT
CTGGACCCAGATATGGTGGTGTCACAGCAGGCAGGGACCCCTCCAGGAATGCCTGACCTG
GTGGTTGAACAGGCTTCTGGGCTGTCTGACTGGTGGAACCCTGCCCTGAGGAAACGTATG
CTGAGTGACAGTGGGATGATCACACCCCACTATGAGGACTCAGATCTGAAAGACTTCAGC
CACTCCAGAGTGCTACAGTCCCCAGTCTCTTCAGAAGATCATGCAATTTTGCAGGCAGTC
CTCACCGGTGACCTTATGAAGCTCATGGAGAGCTATAAGAATGGAGGGAGCTTACTAATT
CAGGGACCAGGGCACTGTTCTCTCCTTCACTACGCAGCTAAGACCGGCAATGGGGACATT
GTGAAGTACATCCTTGACCACGGACCTGCAGAATTACTGGATATGGCAGACAGTGAAACG
GGCGAGACTGCACTGCACAAGGCTGCCTGCCAAAGGAACCGGGCTGTATGCCAGCTTCTG
GTGGATGCAGGAGCATCTCTGAGACAGACAGACTCCAAGGGCAAGACACCCCAGGAACGA
GCACAGCAGGCCGGGGACCCAGATTTGGCTGCTTACCTAGAAAGCCGACAGAACTATAAG
ATCATTGGCCATGAAGACCTGGAAACTGCTGTT

Protein sequence predicted based on nucleotide sequence

MDAAGRGCHLLPLPAARGPARAPAASSALSPTGLCSGTTSASFAAAGAVAMNPSSSAGEE
RGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPAAAGQKEKEEALEEKLRDL
TFRKQVSYRKAISRTGLQHLAPAHPLGLPVANGPAKEPRATLDWSENAVNGEHLWLETNV
SGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPR
ENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHI
EEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQETKGRPFV
IKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALEMYRKVP
NLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKI
LCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHE
SREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQDLKFQCI
VFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCR
EVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQAADL
RRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGLHYDKDKLREASIPLGILVVRGDCDL
ETCRMYIDRLQEDLQSVSSGSQRVHYQDQETSFPRALSAQRLSPRWCFLDATSADRFYRI
DRSQEHLHFVMEISHDEIFILDPDMVVSQQAGTPPGMPDLVVEQASGLSDWWNPALRKRM
LSDSGMITPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVLTGDLMKLMESYKNGGSLLI
QGPGHCSLLHYAAKTGNGDIVKYILDHGPAELLDMADSETGETALHKAACQRNRAVCQLL
VDAGASLRQTDSKGKTPQERAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 29
End on reference sequence 3244
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable