Sema4b (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  Sema4b
Gene Name:  sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4B
Original Clone ID:
FLH270147.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format: 
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297284
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2469nts
Open reading frame: 1 to 2469

ATGGGCCGGGCGAGCCGCTCCGCTGTCCTGCGGCGCGCGCTGCTGCTGCTGCTGCTACTG
CTGCTGCTGCGGACGACGACTACGCGGGCGCTCGGCCCCCGGATCAGTGTGCCGCTGGGC
TCTGAAGAAAGGCTGATTAGAAAATTTGAAGCTGAAAACATCTCCAACTACACGGCCCTT
CTGCTGAGCCAGGATGGCAAGACGCTGTATGTGGGGGCCCGAGAGGCCCTCTTTGCACTT
AACAGCAACCTCAGCTTCTTGCCAGGCGGGGAGTACCAAGAGCTGCTGTGGAGTGCAGAT
GCTGACAGGAAGCAGCAGTGCAGCTTCAAGGGCAAGGACCCAAAGCGTGACTGTCAAAAC
TACATCAAGATCCTCCTGCCACTCAACAGCAGCCACCTGCTCACCTGTGGCACGGCCGCC
TTCAGCCCCCTGTGTGCTTACATTCACATAGCGAGCTTTACTTTAGCCCAAGATGAGGCC
GGCAATGTCATTCTGGAGGATGGCAAGGGTCGTTGTCCCTTTGACCCCAACTTCAAGTCC
ACGGCTCTGGTGGTTGATGGTGAGCTGTACACTGGAACAGTCAGTAGCTTCCAGGGAAAC
GACCCAGCCATTTCCCGGAGCCAGAGTTCCCGCCCCACCAAGACTGAGAGCTCCCTCAAC
TGGCTACAAGACCCTGCCTTTGTGGCCTCGGCCTACGTCCCCGAGAGCCTGGGCAGCCCC
ATAGGTGATGATGATAAGATCTACTTCTTCTTCAGCGAGACGGGCCAGGAGTTTGAGTTC
TTTGAGAACACCATCGTGTCCCGAGTTGCCCGAGTCTGTAAGGGCGATGAGGGTGGAGAG
CGGGTGTTGCAGCAACGCTGGACCTCCTTTCTCAAGGCTCAGCTCCTGTGCTCCCGGCCT
GATGATGGCTTTCCCTTTAACGTGCTACAAGATGTCTTCACCCTGAACCCCAACCCTCAG
GATTGGCGCAAGACCCTTTTCTATGGGGTCTTTACCTCCCAGTGGCACAGAGGGACCACA
GAAGGCTCTGCCATCTGCGTCTTCACCATGAATGATGTGCAGAAGGCCTTTGACGGCCTG
TACAAGAAAGTAAACAGAGAGACACAGCAGTGGTATACCGAGACCCACCAGGTGCCCACA
CCGCGGCCGGGAGCGTGCATTACCAACAGTGCCCGGGAACGGAAGATCAACTCGTCCCTG
CAGCTCCCAGACCGAGTGCTGAACTTCCTCAAGGATCACTTCTTGATGGATGGGCAGGTC
CGCAGTCGCCTGCTGCTGCTGCAGCCCAGAGCCCGCTACCAGCGTGTGGCTGTGCACCGT
GTGCCTGGCCTGCACAGCACTTATGATGTCCTATTTCTGGGCACTGGTGATGGCCGCCTG
CACAAAGCAGTGACCCTGAGCTCCAGAGTCCACATCATTGAGGAGCTGCAGATCTTCCCT
CAAGGACAGCCTGTGCAGAACCTGCTCTTGGACAGCCATGGGGGACTGTTGTATGCCTCC
TCCCATTCCGGGGTGGTGCAAGTGCCCGTAGCCAACTGCAGCCTGTACCCAACCTGTGGA
GACTGCCTCCTGGCTCGAGACCCCTACTGCGCCTGGACTGGCTCTGCCTGCAGGCTCGCT
AGCCTCTACCAGCCTGATCTGGCCTCCAGGCCATGGACCCAGGACATTGAGGGTGCCAGT
GTCAAGGAACTCTGCAAGAATTCCTCATACAAGGCCCGGTTTCTTGTGCCAGGTAAGCCA
TGTAAACAAGTCCAGATCCAACCAAACACAGTGAACACCCTGGCCTGCCCACTCCTCTCA
AACCTGGCCACTCGGCTCTGGGTGCACAATGGAGCCCCAGTCAATGCCTCTGCCTCCTGC
CGCGTGTTACCCACCGGGGACCTGCTGCTGGTGGGCAGCCAGCAGGGTTTGGGGGTGTTC
CAGTGTTGGTCGATAGAAGAAGGATTCCAGCAGCTTGTGGCCAGCTACTGCCCAGAGGTG
ATGGAGGAGGGGGTAATGGACCAAAAGAACCAGCGTGATGGTACCCCAGTCATTATCAAC
ACATCACGAGTGAGTGCACCGGCTGGTGGCAGGGCCAGCTGGGGTGCGGACAAGTCCTAC
TGGAATGAATTCCTGGTGATGTGTACTCTGTTTGTGTTTGCTATGGTGCTTTTGTTTCTG
TTCTTTCTCTACCGACATCGGGATGGCATGAAACTCTTCCTAAAGCAGGGCGAATGTGCC
AGTGTGCACCCCAAGACTCGCCCTATAGTGCTACCACCTGAGACCCGACCGCTGAATGGT
GTCGGCCCTCCTAGCACCCCACTTGACCACCGAGGCTACCAGGCTCTGTCGGATAGCTCC
CCAGGGCCCAGAGTCTTCACTGAATCAGAGAAGAGGCCACTGAGCATCCAGGACAGCTTT
GTAGAGGTGTCTCCCGTGTGTCCCCGGCCCCGAGTTCGACTGGGCTCTGAGATCCGAGAC
TCTGTGGTA

Protein sequence predicted based on nucleotide sequence

MGRASRSAVLRRALLLLLLLLLLRTTTTRALGPRISVPLGSEERLIRKFEAENISNYTAL
LLSQDGKTLYVGAREALFALNSNLSFLPGGEYQELLWSADADRKQQCSFKGKDPKRDCQN
YIKILLPLNSSHLLTCGTAAFSPLCAYIHIASFTLAQDEAGNVILEDGKGRCPFDPNFKS
TALVVDGELYTGTVSSFQGNDPAISRSQSSRPTKTESSLNWLQDPAFVASAYVPESLGSP
IGDDDKIYFFFSETGQEFEFFENTIVSRVARVCKGDEGGERVLQQRWTSFLKAQLLCSRP
DDGFPFNVLQDVFTLNPNPQDWRKTLFYGVFTSQWHRGTTEGSAICVFTMNDVQKAFDGL
YKKVNRETQQWYTETHQVPTPRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQV
RSRLLLLQPRARYQRVAVHRVPGLHSTYDVLFLGTGDGRLHKAVTLSSRVHIIEELQIFP
QGQPVQNLLLDSHGGLLYASSHSGVVQVPVANCSLYPTCGDCLLARDPYCAWTGSACRLA
SLYQPDLASRPWTQDIEGASVKELCKNSSYKARFLVPGKPCKQVQIQPNTVNTLACPLLS
NLATRLWVHNGAPVNASASCRVLPTGDLLLVGSQQGLGVFQCWSIEEGFQQLVASYCPEV
MEEGVMDQKNQRDGTPVIINTSRVSAPAGGRASWGADKSYWNEFLVMCTLFVFAMVLLFL
FFLYRHRDGMKLFLKQGECASVHPKTRPIVLPPETRPLNGVGPPSTPLDHRGYQALSDSS
PGPRVFTESEKRPLSIQDSFVEVSPVCPRPRVRLGSEIRDSVV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence 2758
Start on reference sequence 287
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable