Gene:
Gene Symbol:
Plcb2
Gene Name:
phospholipase C, beta 2
Original Clone ID:
FLH270152.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297289
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3543nts
Open reading frame: 1 to 3543
ATGTCTTTGCTCAACCCTGTTCTATTGCCCCCTAACGTGAAGGCATATCTGAGCCAAGGG
GAGCGCTTCATCAAGTGGGACGATGAAACTTCAATAGCCTCTCCTGTTATCCTCCGTGTG
GATCCCAAGGGCTATTACTTATACTGGACATATCAAAATCAGGAGATGGAGTTCCTGGAT
GTCACGAGTATCCGAGATACTCGCTTTGGGAAGTTTGCAAAGATACCCAAGAGCCAGAAA
CTCCGGGAGGTCTTCAACATGGACTTTCCAGACAACCACTTCCTGCTGAAAACACTCACA
GTGGTGTCCGGCCCTGACATGGTGGACCTCACCTTCTACAACTTTGTCTCTTACAAGGAG
AACGTAGGCAAGGACTGGGCTGAGGACGTGCTAGCTCTCGCCAAACACCCGATGACAGTC
AATGCTCCTCGAAGCACATTCCTGGACAAGATCCTGGTGAAGCTTAAGATGCAGCTCAAC
CCTGAAGGGAAGATTCCCGTGAAGAATTTTTTCCAGATGTTTCCTGCTGATCGAAAACGG
GTGGAAGCTGCCCTCGGTGCTTGTCACCTTGCAAAAGGCAAAAATGATGCTATCAACCCA
GAGGACTTCCCAGAATCCGTGTACAAGAGCTTCCTCATGAGCCTCTGTCCTCGGCCAGAA
ATCGACGAGATCTTCACTTCCTACCACTCTAAAGCTAAGCCCTACATGACCAAGGAACAC
CTGACCAAATTCATCAATCAGAAGCAGCGAGACCCTCGACTCAACTCCTTGCTGTTCCCA
CCAGCCCGGCCTGAGCAAGTTCAAGTGCTCATTGACAAGTACGAACCCAGCGGCATCAAT
GTGCAGAGGGGCCAGCTGTCACCAGAGGGGATGGTCTGGTTTCTCTGTGGACCAGAGAAC
AGTGTGCTGGCCCACGATACACTGCTGATCCACCAAGACATGACACAACCACTGAATCAC
TATTTCATCAACTCCTCACACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTTTCC
TCGGCTGAGATGTACCGCCAGGTGCTGCTGTCTGGCTGCCGGTGTGTAGAGTTAGACTGT
TGGAAGGGCAAGCCCCCTGACGAAGAGCCCATTATCACCCACGGCTTCACCATGACCACA
GATATCTTGTTCAAGGAAGCAATTGAAGCCATTGCAGAAAGTGCCTTTAAGACCTCCCCG
TATCCTGTCATCCTATCATTTGAAAACCATGTAGACTCACCCCGGCAACAGGCTAAGATG
GCTGAGTACTGCAGGAGCATGTTTGGAGAGACCTTGCTCACAGATCCCCTGGAAAATTTC
CCTCTGAAACCTGGCATCCCTCTGCCCAGCCCCGAGGACCTCCGGGGCAAGATCCTCATT
AAGAATAAGAAGAACCAGTTTTCTGGGCCAGCATCCCCCAGCAAGAAGCCTGGTGGGGTG
GCTGAGGGCAGCCTCCCGTCTAGTGTCCCTGTAGAAGAGGACACGGGGTGGACTGCTGAG
GACCGGACTGAGGTGGAGGAGGAGGAGGTGGTGGAAGAGGAGGAAGAGGAGGAGTCAGGA
AACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCGGATGAGGGCACAGCAGGCCTGGAG
GTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTATATCCAGCCCACCAAGTTTATC
TCCTTCGAGTTCTCTGCACAGAAGAACAGAAGTTACGTTGTCTCTTCCTTCACGGAGCTC
AAGGCCTACGAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAG
ATGAGCCGTGTGTACCCCAAGGGCACACGCATGGACTCTTCCAACTACATGCCCCAGATG
TTCTGGAATGCTGGATGTCAGATGGTTGCCCTCAATTTCCAGACAATGGACCTGCCCATG
CAGCAGAACATGGCACTGTTTGAGTTCAACGGGCAGAGTGGCTACCTCCTAAAGCACGAG
TTCATGCGCAGACTGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCATTGATGTGGTG
GTAGCCACCACCCTTTCCATTACGATCATCTCTGGGCAGTTCCTGTCAGAGCGCAGTGTA
CGCACCTATGTGGAAGTGGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGGTATCGA
ACCAAGCTGTCACCTACTGCTAACTCCATCAATCCTGTCTGGAAAGAGGAACCCTTTATC
TTTGAGAAGATCTTGATGCCTGAGCTGGCCTCCCTCAGGATAGCTGTGATGGAAGAAGGC
AGCAAATTTCTTGGACACCGCATCATCCCCATCAATGCCTTACATTCTGGATACCACCAT
CTGTGCCTGCGCAGTGAGAGCAACATGGCCCTTACCATGCCAGCTCTCTTTGTCTTCCTG
GAGATGAAGGACTACATACCTGACACCTGGGCAGATCTTACAGTAGCCCTTGCCAACCCC
ATCAAGTACTTCAATGCCCAGGATAAGAAGTCCGTGAAGCTCAAAGGAGTGACAGGAAGT
CTGCCCGAGAAACTCTTCTCTGGGACACCTGTTGCCAGTCAGTCCAATGGGGCGCCAGTC
TCGGCAGGCAATGGGTCAACAGCGCCTGGGACCAAGGCCACGGGGGAAGAAGCTACGAAA
GAAGTGACAGAGCCACAGACCGCCAGCTTGGAAGAGTTGCGGGAACTGAAGGGCGTGGTG
AAACTGCAGCGAAGACATGAGAAGGAGCTTCGAGAGTTGGAGCGCCGTGGAGCCCGGCGC
TGGGAGGAACTGCTGCAGCGTGGCGCCGCACAGCTGGCGGAGCTCCAGACCCAGGCAGCA
GGCTGCAAGCTCCGCCCAGGCAAGGGCTCCCGCAAGAAGAGGACCCTGCCCTGTGAGGAG
ACCGTCGTGGCGCCTAGCGAGCCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAAG
GACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCCGTCCTCAAG
CGCAAGGAGCAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAA
CAGGCTGCTGAACTCAAGACCTTCAAGGAGACCTCAGAAACTGACACCAAAGAAATGAAG
AAAAAACTGGAGGCCAAGAGGCTGGAACGGATCCAAGCCATGACCAAGGTCACCACAGAC
AAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTAACAACTCCCACATCCAGGAAGTGGTC
CAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGAGAAGCTGGAAGAGAGG
CAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAGGCGCTG
GCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCGGTGCGGGCC
TACTTCAAGGACTGCTTCCCCACCGAAGCAGAAGACAAGCCTGAGAGGTCCTGTGAGGCC
TCTGAGGAGTCGTGTCCACAGGAACCACTTGTGAGCAAGGCAGACACTCAGGAGAGCCGC
CTC
Protein sequence predicted based on nucleotide sequence
MSLLNPVLLPPNVKAYLSQGERFIKWDDETSIASPVILRVDPKGYYLYWTYQNQEMEFLD VTSIRDTRFGKFAKIPKSQKLREVFNMDFPDNHFLLKTLTVVSGPDMVDLTFYNFVSYKE NVGKDWAEDVLALAKHPMTVNAPRSTFLDKILVKLKMQLNPEGKIPVKNFFQMFPADRKR VEAALGACHLAKGKNDAINPEDFPESVYKSFLMSLCPRPEIDEIFTSYHSKAKPYMTKEH LTKFINQKQRDPRLNSLLFPPARPEQVQVLIDKYEPSGINVQRGQLSPEGMVWFLCGPEN SVLAHDTLLIHQDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDC WKGKPPDEEPIITHGFTMTTDILFKEAIEAIAESAFKTSPYPVILSFENHVDSPRQQAKM AEYCRSMFGETLLTDPLENFPLKPGIPLPSPEDLRGKILIKNKKNQFSGPASPSKKPGGV AEGSLPSSVPVEEDTGWTAEDRTEVEEEEVVEEEEEEESGNLDEEEIKKMQSDEGTAGLE VTAYEEMSSLVNYIQPTKFISFEFSAQKNRSYVVSSFTELKAYELLSKASMQFVDYNKRQ MSRVYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMALFEFNGQSGYLLKHE FMRRLDKQFNPFSVDRIDVVVATTLSITIISGQFLSERSVRTYVEVELFGLPGDPKRRYR TKLSPTANSINPVWKEEPFIFEKILMPELASLRIAVMEEGSKFLGHRIIPINALHSGYHH LCLRSESNMALTMPALFVFLEMKDYIPDTWADLTVALANPIKYFNAQDKKSVKLKGVTGS LPEKLFSGTPVASQSNGAPVSAGNGSTAPGTKATGEEATKEVTEPQTASLEELRELKGVV KLQRRHEKELRELERRGARRWEELLQRGAAQLAELQTQAAGCKLRPGKGSRKKRTLPCEE TVVAPSEPHDRADPRVQELKDRLEQELQQQGEEQYRSVLKRKEQHVTEQIAKMMELAREK QAAELKTFKETSETDTKEMKKKLEAKRLERIQAMTKVTTDKVAQERLKREINNSHIQEVV QAVKQMTETLERHQEKLEERQTACLEQIQAMEKQFQEKALAEYEAKMKGLEAEVKESVRA YFKDCFPTEAEDKPERSCEASEESCPQEPLVSKADTQESRL
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
118
End on reference sequence
3663
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
