Gene:
Gene Symbol:
Adcy8
Gene Name:
adenylate cyclase 8
Original Clone ID:
FLH270155.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297292
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3747nts
Open reading frame: 1 to 3747
ATGGAGCTCTCGGATGTGCACTGCCTTAGTGGCAGCGAGGAACTCTACACCATCCAACCG
ACGCCCCCGGCCGGCGACGACGGGAGCGGCTCTCGGCCGCAGCGGCTGCTGTGGCAGACG
GCGGTGCGGCACATCACGGAGCAGCGCTTCATCCACGGGCACCGAGGCGGCGGCGGCGGC
GGGGTCTCCCGCAAAGCCTCGAACCCTGCGGGCAGTGGACCCAATCACCACGCGCCGCAG
CTGTCTAGCGACTCGGTGCTGCCTCTCTATTCTCTGGGCCCCGGAGAGCGAGCGCACAAC
ACCGGTGGCACCAAAGTCTTTCCGGAACGCAGCGGGAGCGGCAGTGCCAGTGGCAGCGGG
GGCGGGGGCGACTTGGGCTTCCTACACCTTGACTGTGCCCCAAGTAACTCGGATTTCTTC
CTCAATGGAGGATACAGCTACCGAGGGGTCATTTTTCCAACCCTACGCAACTCCTTCAAG
TCTCGGGATCTGGAGCGCCTCTACCAACGCTATTTCCTGGGCCAGAGGCGCAAATCGGAG
GTAGTGATGAACGTGTTGGATGTACTAACCAAACTCACCCTTTTAGTCCTGCACTTGAGC
CTGGCCTCGGCTCCAATGGACCCTCTCAAGGGCATCCTGCTAGGCTTTTTCACTGGCATC
GAGGTGGTGATCTGCGCCCTCGTGGTGGTCAGGAAGGACAACACCTCCCACACTTACCTG
CAATACAGCGGCGTGGTCACTTGGGTGGCTATGACCACCCAGATTCTGGCAGCAGGCCTG
GGCTATGGGCTTCTGGGCGACGGCATAGGCTACGTGCTTTTTACACTCTTCGCCACCTAC
AGCATGCTTCCCCTGCCTCTCACCTGGGCCATCTTGGCCGGCCTGGGCACATCTTTGCTG
CAAGTCACCCTTCAAGTACTCATACCCAGGCTAGCGGTCTTTTCCATCAACCAGGTCCTG
GCCCAGGTGGTGCTATTCATGTGTATGAATACAGCAGGAATCTTCATCAGTTACCTTTCA
GACCGTGCCCAGCGCCAGGCCTTCCTGGAGACCCGGAGGTGTGTGGAGGCCAGGCTCCGC
TTGGAGACAGAGAACCAAAGACAGGAGCGGCTTGTGCTGTCTGTGCTCCCCAGGTTTGTC
GTCCTGGAAATGATCAACGACATGACCAATGTGGAGGATGAGCACCTGCAGCATCAGTTC
CACCGCATCTACATCCATCGCTATGAGAACGTCAGTATTCTTTTTGCAGATGTCAAAGGA
TTTACCAACCTCTCTACGACCTTGTCTGCTCAGGAGCTGGTCAGGATGCTCAACGAACTC
TTTGCCAGATTCGACCGGCTGGCCCATGAGCATCACTGTCTTCGCATTAAAATCCTGGGG
GACTGCTACTACTGTGTGTCTGGACTGCCTGAGCCCCGCCGGGACCACGCTCATTGCTGT
GTTGAAATGGGCCTCAGCATGATCAAAACTATCAGGTTTGTGAGATCCAGAACGAAGCAC
GATGTTGACATGCGAATTGGAATCCATTCAGGTTCTGTGCTATGTGGTGTGTTGGGCCTG
AGGAAATGGCAGTTTGATGTCTGGTCTTGGGATGTGGACATAGCAAACAAGCTTGAGTCT
GGAGGAATCCCTGGGAGGATTCACATTTCTAAAGCCACCCTGGATTGCCTCAATGGTGAC
TATAACGTGGAAGAGGGTCACGGGAAAGAGAGGAACGAATTCTTGAGGAAGCATAACATA
GAGACCTATTTGATTAAGCAGCCTGAGGAGAGTTTGCTCTGCTTGCCTGAAGACATCGTT
AAGGAGTCGGTGAGCTGCTCTGACAGGAGAAACAGTGGAGCAACATTCACAGAAGGATCG
TGGAGCCCAGAGCTGCCTTTCGACAACATCGTGGGCAAACAGAATACTCTGGCTGCCCTA
ACAAGAAATTCAATAAATCTGCTTCCAAACCATCTCGCACAAGCTTTGCATGTCCAGTCT
GGGCCTGAGGAAATTAACAAGAGAATAGAGCATACCATCGACTTGCGGAGTGGCGATAAG
TTGAGAAGAGAGCATATCAAGCCATTCTCACTGATGTTTAAAGACTCCAGCCTGGAGCAC
AAGTATTCTCAAATGAGGGATGAAGTATTCAAGTCAAACTTGGTCTGTGCATTTATCGTT
CTTCTGTTTATCACTGCAATTCAAAGTTTGCTTCCTTCTTCAAGGCTGATGCCTATGACT
ATCCAGTTCTCCATCCTGATCATGCTGCACTCTGCCCTGGTCCTCATCACCACAGCAGAA
GACTATAAGTGTCTGCCCCTCATCCTCCGAAAAACCTGCTGTTGGATTAATGAGACCTAT
CTGGCCCGCAACGTCATCATCTTCGCTTCCATCTTAATTAACTTCCTGGGAGCTGTCCTA
AATATTCTGTGGTGTGATTTTGACAAGTCGATACCCTTGAAGAACCTGACTTTCAATTCC
TCAGCTGTGTTTACAGATATCTGCTCCTACCCAGAGTACTTTGTCTTCACTGGGGTGTTG
GCCATGGTGACCTGTGCTGTGTTTCTACGGCTTAACTCTGTCCTGAAGCTGGCAGTGCTA
CTCATCATGATCGCCATCTACGCCCTGCTCACAGAGACCATCTACGCAGGTCTCTTTCTG
AGTTATGACAACCTGAACCACAGTGGAGAAGATTTCCTGGGGACCAAGGAAGCATCACTG
CTGCTGATGGCCATGTTCCTTCTTGCTGTGTTCTACCATGGACAACAGCTGGAGTACACA
GCCCGCCTGGATTTCCTGTGGCGCGTACAGGCCAAAGAGGAGATCAACGAGATGAAGGAA
CTGAGGGAACACAACGAGAACATGCTGCGCAATATCTTACCCAGCCATGTGGCCCGCCAC
TTCCTGGAGAAAGACAGAGACAATGAGGAGCTGTATTCTCAATCCTACGATGCTGTTGGA
GTGATGTTCGCCTCCATCCCTGGGTTCGCAGACTTCTACTCTCAGACAGAAATGAACAAC
CAGGGAGTAGAATGTCTGCGCTTGCTGAATGAGATCATTGCTGACTTTGATGAGCTGCTC
GGGGAGGACCGGTTTCAGGACATTGAGAAGATTAAGACCATTGGTAGTACATACATGGCT
GTCTCAGGACTGTCCCCAGAGAAACAGCAATGTGAAGATAAATGGGGACATTTGTGTGCC
CTGGCTGACTTCTCTCTTGCTCTGACTGAAAGCATACAAGAGATCAACAAGCATTCATTC
AACAATTTTGAACTCCGAATCGGCATCAGCCATGGCTCAGTGGTGGCAGGTGTAATTGGA
GCTAAGAAACCACAGTATGACATTTGGGGGAAAACTGTGAACTTGGCAAGCCGAATGGAC
AGCACGGGAGTAAGTGGCCGGATCCAAGTTCCTGAGGAGACCTACCTTATCCTGAAGGAC
CAGGGCTTTGCCTTTGACTACCGTGGGGAGATATATGTGAAGGGCATCAGTGAACAAGAA
GGGAAAATCAAAACATACTTTCTCCTGGGACGAGTCCAACCCAACCCATTCATCTTACCC
CCAAGGAGACTTCCCGGGCAATACTCTCTGGCTGCGGTTGTCCTTGGCCTTGTCCAGTCT
CTCAACAGGCAAAGGCAGAAGCAACTTCTCAACGAGAACAGCAATTCGGGCATCATCAAG
AGCCATTACAACCGGCGGACTTTGCTAACGCCAAGTGGGCCAGAGCCTGGAGCACAAGCT
GAAGGCACTGACAAATCCGATTTGCCA
Protein sequence predicted based on nucleotide sequence
MELSDVHCLSGSEELYTIQPTPPAGDDGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGG GVSRKASNPAGSGPNHHAPQLSSDSVLPLYSLGPGERAHNTGGTKVFPERSGSGSASGSG GGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSE VVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALVVVRKDNTSHTYL QYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLL QVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLR LETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKG FTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRRDHAHCC VEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLES GGIPGRIHISKATLDCLNGDYNVEEGHGKERNEFLRKHNIETYLIKQPEESLLCLPEDIV KESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQS GPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIV LLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETY LARNVIIFASILINFLGAVLNILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVL AMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASL LLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILPSHVARH FLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELL GEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSF NNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKD QGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQS LNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1192
End on reference sequence
4941
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
