Gene:
Gene Symbol:
Agap2
Gene Name:
ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Original Clone ID:
FLH270168.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297305
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3558nts
Open reading frame: 1 to 3558
ATGAGCCGGGGCGCGGGCGCGCTTCAGCGCCGGACAACGACCTACCTCATCTCGCTGACC
CTGGTCAAGCTCGAGTCGGTACCTCCGCCGCCGCCTTCTCCATCTGCAGCCGCAGCCGGC
GCCCCCGGGGCCAGAGGCTCCGAGCCCCGAGATCCTGGCAGCCCCCGAGGCTCGGAGGAA
CCCGGCAAGAAGCGGCACGAGCGTCTCTTCCACCGGCAGGATGCGCTGTGGATCAGTACC
AGCAGCGCAGGCACCGGGGGTGCGGAGCCCCCCGCGCTGTCCCCGGCTCCGGCCAGTCCG
GCTCGCCCGGTCTCCCCGGCTCCGGGCCGCCGACTGTCCCTATGGGCCGCCCCTCCGGGA
CCCCCGCTCTCCGGGGGGCTGAGCCCGGACTCCAAGCCGGGAGGCGCCCCCTCTTCCTCC
CGGCGCCCTCTACTCAGCAGCCCGAGCTGGGGGGGTCCGGAACCTGAAGGCCGGACGGGT
GGCGGAGTCCCGGGCTCGTCCTCCCCGCATCCTGGCACCGGTAGCCGAAGGCTCAAGGTG
GCGCCTCCTCCACCTGCTCCCAAGCCTTTCAAGACCGTGACCACTAGTGGAGCCAAAGCC
GGCGGGGGCAAGGGCGCAGGCAGCCGCCTGTCATGGCCCGAAAGCGAGGGCAAACCCAGG
GTCAAGGGGTCAAAGAGCACCGCCGGGACTGGAGCTTCTGCCGCTGCGGCCGGCGGGGGA
GGGAGCGCTGCAGTCACGACCTCTGGTGGGGTCGGGGCTGGGGCTGGAACCAGAGGGAAG
TTGTCCCCTCGGAAAGGCAAGAGTAAGACCTTGGACAATAGTGACTTACACCCAGGACCC
AGTGCCGGCTCTCCTCCGCTCACCGTGCCAGCAATCCCAGTTCCAGCCACTTCTGTCACT
GCCACCTCCACGCAGCCCCTCGGGCCTGCACCCCCTATCACTCTGGAGCCACCAGCTCCA
GGGCTGAAAAGGGGCCGGGAGGGCGGCCGAGCGTCCACTCGTGACCGCAAGATGCTCAAG
TTTATCAGCGGTATCTTCACCAAGAGCACAGGAGGGCCTCCTGGTCCAGGGCCCCTTCCC
GGACCCCAAGGCTTGTCTTCCAGCAGCGGGTCCAGGGAGCTGCTGGGAGCAGAACTACGC
ACCTCCCCTAAGGCTGTGGTTAATAGCCAGGAATGGACTTTGAGCCGATCAATTCCCGAA
CTGCGCCTGGGTGTGCTGGGTGATGTCAGGAGTGGGAAGTCGTCCCTCATCCATAGATTC
CTCACGGGTTCATACCAGGTGCTGGAGAAGATTGAGAGTGAGCAGTACAAGAAGGAGATG
CTAGTGGATGGGCAGACCCATCTGGTGCTGATCCGGGAGGAAGCAGGGGCACCCGATGCC
AAGTTCTCAGGCTGGGCAGATGCTGTGATCTTCGTCTTCAGCCTGGAGGATGAGAGCAGC
TTCCAGGCTGTGAGCCGTCTCCATGGGCAGCTGAGCTCCCTCCGGGGGGAGGGGCGAGGA
GGTCTGGCCCTGGCTCTGGTTGGGACCCAAGACAGGATCAGTGCATCATCTCCTCGAGTG
GTGGGTGATGCTCGAGCCAGGGCTCTGTGCACGGACATGAAGCGCTGTAGCTACTACGAG
ACCTGCGCAACCTATGGGCTCAATGTGGACCGGGTCTTCCAGGAGGTGGCCCAGAAGGTG
GTGACCTTGCGCAAACAACAACAACTCCTGGCTGCTTGCAAATCCCTGCCTAGCTCGCCA
AGCCATTCAGCTGCATCTACTCCTGTAGCTGGGCAGGCTAGTAACGGGGGCCACACTAGC
GACTACTCTTCTTCCCTCCCATCCTCACCCAACGTTGGACACCGGGAGCTCCGAGCTGAG
GCAGCTGCGGTGGCTGGGTTGAGTACCCCAGGGTCCTTACACAGAGCAGCCAAGCGGCGG
ACCAGCCTTTTTGCGAACCGTCGGGGCAGCGATGCTGAGAAGCGGAGCTTAGACAGCCGG
GGAGAGACAACAGGGAGTGGGCGAGCCATCCCCATCAAACAGAGCTTCCTGCTAAAACGA
AGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTGACCTTGTCCAGCAATGGC
TTTCTACTCTACCACCCCAGCATTAATGACTACATCCACAGTACACATGGCAAAGAGATG
GACTTGTTACGAACAACGGTCAAAGTCCCAGGCAAGCGGCCCCCAAGGGCTATCTCTGCT
TTCGGCCCCTCAGCCAGCATCAACGGGTTGGTCAAGGATATGAGCACCGTCCAGATGGGT
GAAGGCCCTGAAGCCTCTACTCCCATGCCAAGCCCCAGCCCTAGCCCCAGTTCCCTGCAG
CTTCCAACAGACCAGACATCCAAGCACCTCTTGAAGCCAGACCGGAATTTGGCCCGTGCC
CTTAGCACCGACTGTACCCCATCTGGAGATCTGAGTCCCCTGAGTCGGGAACCCCCACCT
TCGCCCATGGTGAAGAAGCAGAGGAGGAAGAAACTGTCCACGCCTTCTAAGACTGAAGGC
TCGGCTGTGCAAGCTGAAGCCAAGCGCAAAATGTGGAAACTAAAATCCTTTGGTAGTTTA
AGAAATATTTATAAAGCAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACTGGTCAG
ACGTGGCACTTTGAGGCAGCCAGCTTTGAGGAGCGGGATGCTTGGGTTCAGGCCATTGAG
AGTCAGATCCTGGCCAGTCTGCAGTGCTGTGAGAGCAGCAAGGTCAAGCTGCGCACTGAC
AGCCAAAGCGAAGCTGTGGCCATCCAGGCGATCCGCAACGCAAAGGGAAACTCGACCTGT
GTGGACTGTGGAGCTCCCAACCCCACGTGGGCCAGTTTGAATCTGGGCGCACTCATCTGC
ATCGAGTGTTCTGGCATCCACCGGAACCTGGGCACACACCTGTCCCGAGTGCGTTCACTG
GATTTGGATGACTGGCCGCGGGAGCTGACCCTGGTGCTGACGGCCATTGGCAACGACACA
GCCAACCGCGTGTGGGAGAGCGACACTCGAGGCCGTGCCAAGCCTACACGGGACTCTTCG
CGGGAGGAGCGTGAGTCGTGGATTCGCGCCAAGTATGAGCAGCTGCTGTTCCTGGCGCCT
CTGGGCACCACGGAGGAGCCGCTGGGCCGCCAACTGTGGGCCGCTGTGGAGGCCCAGGAC
GTGGCCGCCGTTCTGCTGCTTCTGGCCCACGCGCGACATGGGCCGCTCGACACCAGCGTG
GAGGACCCGCAGCTCCGCTCGCCGCTGCACTTGGCGGCCGAGCTCGCCCACGTTGTCATT
ACACAGCTGCTGTTGTGGTATGGCGCAGACGTGGCGGCCCGCGATGCGCAAGGCCGCACG
GCACTGTTCTATGCCCGCCAGGCTGGCAGCCAGCTGTGTGCAGACATCCTTCTCCAGCAC
GGCTGCCCGGGAGAGGGCGGCAGCACGGCCACCACGCCCAGCGCGGCCACCACCCCCAGC
ATTACCGCCACGCCCAGCCCGAGGCGCCGGAGCAGTGCTGCCAGCTTGGGCCGCGTGGAC
ACCACGATCGCGCTGGTA
Protein sequence predicted based on nucleotide sequence
MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAPGARGSEPRDPGSPRGSEE PGKKRHERLFHRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAAPPG PPLSGGLSPDSKPGGAPSSSRRPLLSSPSWGGPEPEGRTGGGVPGSSSPHPGTGSRRLKV APPPPAPKPFKTVTTSGAKAGGGKGAGSRLSWPESEGKPRVKGSKSTAGTGASAAAAGGG GSAAVTTSGGVGAGAGTRGKLSPRKGKSKTLDNSDLHPGPSAGSPPLTVPAIPVPATSVT ATSTQPLGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISGIFTKSTGGPPGPGPLP GPQGLSSSSGSRELLGAELRTSPKAVVNSQEWTLSRSIPELRLGVLGDVRSGKSSLIHRF LTGSYQVLEKIESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDESS FQAVSRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCTDMKRCSYYE TCATYGLNVDRVFQEVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTS DYSSSLPSSPNVGHRELRAEAAAVAGLSTPGSLHRAAKRRTSLFANRRGSDAEKRSLDSR GETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYVTLSSNGFLLYHPSINDYIHSTHGKEM DLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGPEASTPMPSPSPSPSSLQ LPTDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLSTPSKTEG SAVQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIE SQILASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSTCVDCGAPNPTWASLNLGALIC IECSGIHRNLGTHLSRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPTRDSS REERESWIRAKYEQLLFLAPLGTTEEPLGRQLWAAVEAQDVAAVLLLLAHARHGPLDTSV EDPQLRSPLHLAAELAHVVITQLLLWYGADVAARDAQGRTALFYARQAGSQLCADILLQH GCPGEGGSTATTPSAATTPSITATPSPRRRSSAASLGRVDTTIALV
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
4277
Start on reference sequence
717
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
