Gene:
Gene Symbol:
Nell1
Gene Name:
NEL-like 1
Original Clone ID:
FLH270171.01L
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
FUSION
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297308
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2430nts
Open reading frame: 1 to 2430
ATGCCGATGGATGTGATTTTAGTTTTGTGGTTCTGTGTGTGCACCGCCAGGACAGTGCTG
GGCTTTGGGATGGACCCTGACCTTCAGATGGACATCATCACTGAACTTGACCTTGTGAAC
ACCACCCTGGGCGTCACTCAGGTGGCTGGACTACACAATGCCAGTAAGGCATTTCTGTTT
CAAGATGTACAGAGAGAGATCCACTCAGCCCCTCATGTGAGTGAGAAGCTGATCCAGCTA
TTCCGGAATAAGAGTGAGTTTACCTTTTTGGCTACAGTGCAGCAGAAGCCGTCCACCTCA
GGGGTGATACTGTCGATCCGGGAGCTGGAACACAGCTATTTTGAACTGGAGAGCAGTGGC
CCAAGAGAAGAGATACGCTATCATTACATCCATGGCGGCAAGCCCAGGACTGAGGCCCTT
CCCTACCGCATGGCCGATGGACAGTGGCACAAGGTCGCGCTGTCTGTGAGCGCCTCTCAC
CTCCTACTCCATGTCGACTGCAATAGGATTTATGAGCGTGTGATAGATCCTCCGGAGACC
AACCTTCCTCCAGGAAGCAATCTATGGCTTGGGCAACGTAATCAAAAGCATGGCTTTTTC
AAAGGAATCATCCAAGATGGCAAGATCATCTTCATGCCGAACGGCTTCATCACACAGTGC
CCCAACCTAAATCGCACTTGCCCAACATGCAGTGATTTCCTGAGCCTGGTTCAAGGAATA
ATGGATTTGCAAGAGCTTTTGGCCAAGATGACTGCAAAACTGAATTATGCAGAGACGAGA
CTTGGTCAACTGGAAAATTGCCACTGTGAGAAGACCTGCCAAGTGAGTGGGCTGCTCTAC
AGGGACCAAGACTCCTGGGTAGATGGTGACAACTGCAGGAACTGCACATGCAAAAGTGGT
GCTGTGGAGTGCCGAAGGATGTCCTGTCCCCCACTCAACTGTTCCCCAGACTCACTTCCT
GTGCATATTTCTGGCCAATGTTGTAAAGTTTGCAGACCAAAATGTATCTATGGAGGAAAA
GTTCTTGCTGAGGGCCAGCGGATTTTAACCAAGACCTGCCGGGAATGTCGAGGTGGAGTC
TTGGTAAAAATCACAGAAGCTTGCCCTCCTTTGAACTGCTCAGAGAAGGATCATATTCTT
CCGGAGAACCAGTGCTGCAGGGTCTGCCGAGGTCATAACTTCTGTGCAGAAGCACCTAAG
TGTGGAGAAAACTCGGAATGCAAAAATTGGAATACAAAAGCGACTTGTGAGTGCAAGAAT
GGATACATCTCTGTCCAGGGCAACTCTGCATACTGTGAAGATATCGATGAGTGTGCAGCA
AAGATGCACTACTGTCATGCCAACACGGTGTGTGTCAACTTGCCGGGGTTATATCGCTGT
GACTGCATCCCAGGATACATCCGTGTGGATGACTTCTCTTGTACGGAGCATGATGATTGT
GGCAGCGGACAACACAACTGTGACAAAAATGCCATCTGTACCAACACAGTCCAGGGACAC
AGCTGTACCTGCCAGCCAGGCTACGTGGGAAATGGTACTGTCTGCAAAGCATTCTGTGAA
GAGGGTTGCAGATACGGAGGTACCTGTGTGGCCCCTAACAAATGTGTCTGTCCTTCTGGA
TTCACAGGAAGCCACTGTGAGAAAGATATTGATGAATGTGCAGAGGGATTCGTTGAGTGC
CACAACCACTCCCGCTGCGTTAACCTTCCAGGGTGGTACCACTGTGAGTGCAGAAGCGGT
TTCCATGACGATGGGACCTATTCACTGTCCGGGGAGTCCTGCATTGATATTGATGAATGT
GCCTTAAGAACTCACACTTGTTGGAATGACTCTGCCTGCATCAACTTAGCAGGAGGATTT
GACTGCCTGTGTCCCTCTGGGCCCTCCTGCTCTGGTGACTGTCCCCACGAAGGGGGGCTG
AAGCATAATGGGCAGGTGTGGATTCTGAGAGAAGACAGGTGTTCAGTCTGTTCCTGTAAG
GATGGGAAGATATTCTGCCGGCGGACAGCTTGTGATTGCCAGAATCCAAATGTTGACCTT
TTCTGCTGCCCAGAGTGTGACACCAGGGTCACTAGCCAATGTTTAGATCAAAGCGGACAG
AAGCTCTATCGAAGTGGAGACAACTGGACCCACAGCTGCCAGCAGTGCCGATGTCTGGAA
GGAGAGGCAGACTGCTGGCCTCTAGCTTGCCCTAGTTTGAGCTGTGAATACACAGCCATC
TTTGAAGGAGAGTGTTGTCCCCGCTGTGTCAGTGACCCCTGCCTGGCTGATAATATTGCC
TATGACATCAGAAAAACTTGCCTGGACAGCTCTGGTATTTCGAGGCTGAGCGGCGCAGTG
TGGACAATGGCTGGATCTCCCTGTACAACCTGTCAATGCAAGAATGGGAGAGTCTGCTGC
TCTGTGGATCTGGTGTGTCTTGAGAATAAC
Protein sequence predicted based on nucleotide sequence
MPMDVILVLWFCVCTARTVLGFGMDPDLQMDIITELDLVNTTLGVTQVAGLHNASKAFLF QDVQREIHSAPHVSEKLIQLFRNKSEFTFLATVQQKPSTSGVILSIRELEHSYFELESSG PREEIRYHYIHGGKPRTEALPYRMADGQWHKVALSVSASHLLLHVDCNRIYERVIDPPET NLPPGSNLWLGQRNQKHGFFKGIIQDGKIIFMPNGFITQCPNLNRTCPTCSDFLSLVQGI MDLQELLAKMTAKLNYAETRLGQLENCHCEKTCQVSGLLYRDQDSWVDGDNCRNCTCKSG AVECRRMSCPPLNCSPDSLPVHISGQCCKVCRPKCIYGGKVLAEGQRILTKTCRECRGGV LVKITEACPPLNCSEKDHILPENQCCRVCRGHNFCAEAPKCGENSECKNWNTKATCECKN GYISVQGNSAYCEDIDECAAKMHYCHANTVCVNLPGLYRCDCIPGYIRVDDFSCTEHDDC GSGQHNCDKNAICTNTVQGHSCTCQPGYVGNGTVCKAFCEEGCRYGGTCVAPNKCVCPSG FTGSHCEKDIDECAEGFVECHNHSRCVNLPGWYHCECRSGFHDDGTYSLSGESCIDIDEC ALRTHTCWNDSACINLAGGFDCLCPSGPSCSGDCPHEGGLKHNGQVWILREDRCSVCSCK DGKIFCRRTACDCQNPNVDLFCCPECDTRVTSQCLDQSGQKLYRSGDNWTHSCQQCRCLE GEADCWPLACPSLSCEYTAIFEGECCPRCVSDPCLADNIAYDIRKTCLDSSGISRLSGAV WTMAGSPCTTCQCKNGRVCCSVDLVCLENN
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2472
Start on reference sequence
40
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
