Gene:
Gene Symbol:
NLRP8
Gene Name:
NLR family, pyrin domain containing 8
Original Clone ID:
FLH270876.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297335
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGTGACGTGAATCCACCCTCTGA
CACCCCCATTCCCTTTTCATCCTCCTCCACTCACAGTTCTCATATTCCGCCCTGGACATT
CTCTTGCTACCCCGGCTCCCCATGTGAAAATGGGGTCATGCTGTACATGAGAAACGTGAG
CCATGAGGAGCTACAACGGTTCAAGCAGCTCTTACTGACTGAGCTCAGTACTGGCACCAT
GCCCATCACCTGGGACCAGGTCGAGACAGCCAGCTGGGCAGAGGTGGTTCATCTCTTGAT
AGAGCGTTTCCCTGGACGACGCGCTTGGGATGTGACTTCGAACATCTTTGCCATTATGAA
CTGTGATAAAATGTGTGTTGTAGTCCGCAGAGAGATAAATGCCATTCTGCCTACCTTGGA
ACCAGAGGACTTGAATGTGGGAGAAACACAGGTGAATCTGGAGGAAGGAGAATCTGGTAA
AATACGGCGGTATAAATCGAATGTGATGGAAAAGTTTTTCCCCATATGGGACATTACGAC
TTGGCCTGGAAACCAGAGGGACTTCTTCTACCAAGGTGTACACAGGCACGAGGAGTACTT
ACCATGTCTGCTTCTGCCCAAAAGACCCCAGGGTAGACAGCCCAAGACCGTGGCCATACA
GGGAGCTCCTGGGATCGGAAAAACAATCCTGGCCAAAAAGGTGATGTTTGAGTGGGCCAG
AAACAAGTTCTACGCCCACAAGCGCTGGTGTGCTTTCTACTTCCATTGCCAAGAGGTGAA
CCAGACGACAGACCAGAGCTTCTCCGAGCTGATTGAGCAAAAGTGGCCTGGATCTCAGGA
CCTCGTGTCAAAGATTATGTCCAAACCCGACCAACTTCTGCTGCTCTTGGATGGCTTTGA
GGAGCTCACATCTACCCTCATTGACAGACTGGAGGACCTGAGTGAAGACTGGAGGCAGAA
ATTGCCTGGGTCTGTCCTACTGAGCAGTTTGCTGAGCAAAACGATGCTTCCAGAGGCCAC
GCTACTGATCATGATAAGATTTACCTCTTGGCAGACATGCAAGCCCTTGCTGAAATGTCC
CTCTCTCGTAACCCTTCCGGGGTTTAATACGATGGAAAAAATCAAGTATTTCCAGATGTA
TTTTGGACACACAGAGGAGGGAGACCAAGTCTTGAGTTTCGCCATGGAAAACACCATTCT
CTTCTCCATGTGCCGGGTCCCTGTGGTTTGCTGGATGGTCTGCTCTGGTCTGAAACAGCA
AATGGAGAGAGGAAACAATCTCACACAGTCATGTCCAAATGCCACCTCTGTGTTCGTCCG
GTATATTTCTAGCTTGTTTCCCACCAGAGCTGAGAACTTTTCCAGAAAGATCCACCAAGC
ACAACTGGAAGGTCTGTGTCACTTGGCCGCAGACAGCATGTGGCACAGGAAATGGGTGTT
AGGTAAAGAAGATCTTGAGGAAGCCAAGCTGGATCAGACGGGAGTCACCGCCTTCCTTGG
CATGAGTATTCTTCGGAGAATTGCAGGTGAGGAAGACCACTATGTCTTTACCCTCGTGAC
TTTTCAGGAATTTTTTGCGGCCTTGTTTTATGTTCTCTGTTTCCCACAAAGACTCAAAAA
TTTTCATGTGTTGAGCCACGTGAATATCCAGCGCCTGATAGCGAGTCCCAGAGGAAGCAA
AAGCTATCTCTCTCACATGGGACTTTTCTTATTCGGTTTTCTGAACGAGGCCTGCGCTTC
GGCCGTGGAACAGTCATTCCAATGCAAGGTGTCTTTCGGTAATAAGAGGAAACTGCTGAA
AGTCATACCTCTGTTGCATAAATGTGACCCACCTTCTCCGGGCAGTGGGGTCCCGCAGTT
ATTCTACTGTCTGCATGAAATCCGGGAGGAAGCCTTTGTAAGCCAAGCCCTAAATGATTA
TCATAAAGTTGTCTTGAGAATTGGCAACAACAAAGAAGTTCAAGTGTCTGCTTTTTGCCT
GAAGCGGTGTCAATATTTGCATGAGGTGGAACTGACCGTCACCCTGAACTTCATGAACGT
GTGGAAGCTCAGCTCCAGCTCCCATCCTGGCTCTGAAGCGCCAGAGAGCAATGGGCTGCA
TCGTTGGTGGCAAGACTTATGCTCTGTGTTTGCAACGAATGATAAGCTGGAAGTCCTGAC
TATGACCAACAGTGTTTTGGGGCCTCCTTTTTTGAAGGCTCTCGCGGCCGCACTGAGGCA
CCCTCAGTGCAAACTGCAAAAGCTACTCCTAAGGCGTGTGAATAGCACCATGTTGAACCA
GGACTTAATCGGTGTTTTGACGGGGAACCAGCATCTGAGATACTTGGAAATACAACATGT
GGAAGTGGAGTCCAAAGCTGTGAAGCTTCTATGCAGGGTGCTGAGATCCCCCCGGTGCCG
TCTGCAGTGTCTCAGGTTGGAAGACTGCTTGGCCACCCCTAGAATTTGGACTGATCTTGG
CAATAATCTTCAAGGTAACGGGCATCTAAAGACTCTCATACTAAGAAAAAACTCCCTGGA
GAACTGTGGGGCGTATTACCTGTCTGTGGCCCAGCTGGAGAGGCTGTCGATAGAGAACTG
CAACCTTACACAGCTTACTTGTGAAAGCCTTGCCTCCTGTCTCAGGCAGAGTAAGATGCT
GACCCACCTGAGCTTGGCAGAAAACGCCTTGAAAGATGAAGGGGCCAAGCATATTTGGAA
TGCCCTGCCACACCTGAGATGTCCTCTGCAGAGGCTGGTACTGAGAAAGTGTGACTTGAC
CTTTAATTGCTGTCAGGATATGATCTCTGCGCTCTGTAAAAATAAAACCCTGAAAAGTCT
TGACCTAAGTTTTAATAGCCTGAAGGATGATGGGGTGATCCTGCTGTGTGAGGCCCTGAA
GAACCCTGACTGTACATTACAGATCCTGGAGCTGGAAAACTGCCTGTTCACCTCCATCTG
CTGCCAGGCCATGGCTTCCATGCTCCGCAAAAACCAACATCTGAGACATCTGGACTTGAG
CAAGAATGCGATTGGAGTCTATGGTATTCTGACCTTGTGCGAGGCCTTCTCAAGCCAAAA
GAAGAGAGAAGAGGTCATTTTCTGTATTCCTGCCTGGACTCGAATAACTAGCTTCTCCCC
AACTCCTCACCCACCCGACTTCACGGGAAAAAGTGACTGCCTATCCCAGATTAATCCTTA
AGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSDVNPPSDTPIPFSSSSTHSSHIPPWTFSCYPGSPCENGVMLYMRNVSHEELQRFKQLL LTELSTGTMPITWDQVETASWAEVVHLLIERFPGRRAWDVTSNIFAIMNCDKMCVVVRRE INAILPTLEPEDLNVGETQVNLEEGESGKIRRYKSNVMEKFFPIWDITTWPGNQRDFFYQ GVHRHEEYLPCLLLPKRPQGRQPKTVAIQGAPGIGKTILAKKVMFEWARNKFYAHKRWCA FYFHCQEVNQTTDQSFSELIEQKWPGSQDLVSKIMSKPDQLLLLLDGFEELTSTLIDRLE DLSEDWRQKLPGSVLLSSLLSKTMLPEATLLIMIRFTSWQTCKPLLKCPSLVTLPGFNTM EKIKYFQMYFGHTEEGDQVLSFAMENTILFSMCRVPVVCWMVCSGLKQQMERGNNLTQSC PNATSVFVRYISSLFPTRAENFSRKIHQAQLEGLCHLAADSMWHRKWVLGKEDLEEAKLD QTGVTAFLGMSILRRIAGEEDHYVFTLVTFQEFFAALFYVLCFPQRLKNFHVLSHVNIQR LIASPRGSKSYLSHMGLFLFGFLNEACASAVEQSFQCKVSFGNKRKLLKVIPLLHKCDPP SPGSGVPQLFYCLHEIREEAFVSQALNDYHKVVLRIGNNKEVQVSAFCLKRCQYLHEVEL TVTLNFMNVWKLSSSSHPGSEAPESNGLHRWWQDLCSVFATNDKLEVLTMTNSVLGPPFL KALAAALRHPQCKLQKLLLRRVNSTMLNQDLIGVLTGNQHLRYLEIQHVEVESKAVKLLC RVLRSPRCRLQCLRLEDCLATPRIWTDLGNNLQGNGHLKTLILRKNSLENCGAYYLSVAQ LERLSIENCNLTQLTCESLASCLRQSKMLTHLSLAENALKDEGAKHIWNALPHLRCPLQR LVLRKCDLTFNCCQDMISALCKNKTLKSLDLSFNSLKDDGVILLCEALKNPDCTLQILEL ENCLFTSICCQAMASMLRKNQHLRHLDLSKNAIGVYGILTLCEAFSSQKKREEVIFCIPA WTRITSFSPTPHPPDFTGKSDCLSQINP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
3218
Start on reference sequence
72
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
