Gene:
Gene Symbol:
TGM7
Gene Name:
transglutaminase 7
Original Clone ID:
FLH270910.01X
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
HsCD00297369
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATCAGGTGGCAACCTTGCGGCT
TGAGTCTGTCGACCTGCAGAGCTCCAGGAACAACAAGGAGCACCACACGCAGGAGATGGG
CGTCAAGCGGCTCACTGTGCGCCGCGGCCAGCCCTTCTACCTCCGGCTGAGCTTCAGCCG
ACCCTTCCAGTCCCAGAACGACCACATCACCTTTGTGGCTGAGACCGGACCCAAGCCGTC
AGAGCTGCTGGGGACCCGAGCCACATTCTTCCTCACCCGGGTCCAGCCCGGGAATGTCTG
GAGCGCTTCTGATTTCACCATTGACTCCAACTCTCTCCAAGTTTCCCTTTTCACACCAGC
CAATGCAGTTATTGGCCATTACACTCTGAAAATAGAGATCTCTCAGGGCCAAGGTCACAG
TGTGACTTACCCGCTGGGAACTTTCATCCTACTTTTTAACCCTTGGAGTCCAGAGGACGA
CGTCTACCTGCCAAGTGAAATACTGCTGCAGGAGTATATCATGCGAGATTATGGCTTTGT
TTACAAGGGTCATGAAAGATTCATCACCTCCTGGCCCTGGAACTACGGGCAGTTTGAAGA
GGACATCATAGACATCTGCTTTGAGATCCTGAACAAGAGCCTGTATCACTTAAAGAACCC
GGCCAAAGACTGTTCCCAGCGGAACGACGTGGTGTATGTGTGCAGGGTGGTGAGTGCCAT
GATCAACAGCAACGATGACAATGGCGTGCTGCAGGGGAACTGGGGCGAGGACTACTCCAA
AGGGGTCAGTCCTCTGGAGTGGAAGGGCAGTGTGGCCATCCTACAGCAGTGGTCAGCCAG
GGGCGGGCAGCCTGTGAAGTACGGACAGTGCTGGGTCTTCGCCTCTGTTATGTGCACCGT
AATGAGATGCTTAGGTGTTCCAACCCGTGTTGTTTCCAATTTCCGTTCCGCGCACAACGT
GGATAGGAACTTGACCATCGATACGTACTATGACCGAAATGCCGAGATGCTGTCAACTCA
GAAACGAGACAAAATATGGAACTTCCACGTCTGGAATGAGTGCTGGATGATCCGGAAAGA
TCTCCCACCAGGATACAACGGGTGGCAGGTTCTGGACCCCACTCCCCAGCAGACCAGCAG
TGGGCTGTTCTGCTGTGGCCCTGCCTCTGTGAAGGCCATCAGGGAAGGGGATGTCCACCT
GGCCTATGACACCCCTTTTGTGTATGCCGAGGTGAACGCCGATGAAGTCATTTGGCTCCT
TGGGGATGGCCAGGCCCAGGAAATCCTGGCCCACAACACCAGTTCCATCGGGAAGGAGAT
CAGCACTAAGATGGTGGGGTCAGACCAGCGCCAGAGCATCACCAGCTCCTACAAGTACCC
AGAAGGATCCCCTGAGGAGAGAGCTGTCTTCATGAAGGCTTCTCGGAAAATGCTGGGCCC
CCAAAGAGCTTCTTTGCCCTTCCTGGATCTCCTGGAGTCTGGGGGTCTTAGGGATCAGCC
AGCGCAGCTGCAGCTTCACCTGGCCAGGATACCCGAGTGGGGCCAGGACCTGCAGCTGCT
GCTGCGTATCCAGAGGGTGCCAGACAGCACCCACCCTCGGGGGCCCATCGGACTGGTGGT
GCGCTTCTGTGCACAGGCCCTGCTGCATGGGGGTGGTACCCAGAAGCCCTTCTGGAGGCA
CACAGTGCGGATGAACCTGGACTTTGGGAAGGAGACACAGTGGCCGCTCCTCCTGCCCTA
CAGCAATTACAGAAACAAGCTAACGGACGAAAAGCTCATCCGCGTGTCTGGCATCGCGGA
GGTTGAAGAGACAGGGAGGTCCATGCTGGTCCTAAAAGATATCTGTCTGGAGCCTCCCCA
CTTGTCTATTGAGGTGTCTGAGAGGGCTGAGGTGGGCAAGGCGCTGAGAGTCCATGTCAC
CCTCACCAACACCTTAATGGTGGCTCTGAGCAGCTGCACGATGGTGCTGGAAGGAAGCGG
CCTCATCAATGGGCAGATAGCAAAGGACCTTGGGACTCTGGTGGCCGGACACACCCTCCA
AATTCAACTGGACCTCTACCCGACCAAAGCTGGACCCCGCCAGCTCCAGGTTCTCATCAG
CAGCAACGAGGTCAAGGAGATCAAAGGCTACAAGGACATCTTCGTCACTGTGGCTGGGGC
TCCCTAAGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDQVATLRLESVDLQSSRNNKEHHTQEMGVKRLTVRRGQPFYLRLSFSRPFQSQNDHITF VAETGPKPSELLGTRATFFLTRVQPGNVWSASDFTIDSNSLQVSLFTPANAVIGHYTLKI EISQGQGHSVTYPLGTFILLFNPWSPEDDVYLPSEILLQEYIMRDYGFVYKGHERFITSW PWNYGQFEEDIIDICFEILNKSLYHLKNPAKDCSQRNDVVYVCRVVSAMINSNDDNGVLQ GNWGEDYSKGVSPLEWKGSVAILQQWSARGGQPVKYGQCWVFASVMCTVMRCLGVPTRVV SNFRSAHNVDRNLTIDTYYDRNAEMLSTQKRDKIWNFHVWNECWMIRKDLPPGYNGWQVL DPTPQQTSSGLFCCGPASVKAIREGDVHLAYDTPFVYAEVNADEVIWLLGDGQAQEILAH NTSSIGKEISTKMVGSDQRQSITSSYKYPEGSPEERAVFMKASRKMLGPQRASLPFLDLL ESGGLRDQPAQLQLHLARIPEWGQDLQLLLRIQRVPDSTHPRGPIGLVVRFCAQALLHGG GTQKPFWRHTVRMNLDFGKETQWPLLLPYSNYRNKLTDEKLIRVSGIAEVEETGRSMLVL KDICLEPPHLSIEVSERAEVGKALRVHVTLTNTLMVALSSCTMVLEGSGLINGQIAKDLG TLVAGHTLQIQLDLYPTKAGPRQLQVLISSNEVKEIKGYKDIFVTVAGAP*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2138
Start on reference sequence
6
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
