Gene:
Gene Symbol:
Phip
Gene Name:
pleckstrin homology domain interacting protein
Original Clone ID:
FLH481706.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297387
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 5466nts
Open reading frame: 35 to 5500
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCTCGTGAGAGGAAAGGCCTCTC
GGAGCTGCGATCGGAGCTCTACTTCCTCATCGCCCGGTTCCTGGAAGATGGACCCTGTCA
GCAGGCGGCTCAGGTGCTGATCCGCGAAGTGGCCGAGAAGGAGCTGCTGCCCCGGCGCAC
CGACTGGACCGGGAAGGAGCACCCCAGGACCTACCAGAATCTGGTGAAGTATTATAGACA
CCTTGCACCTGATCACTTGCTGCAAATATGTCATCGGCTAGGACCTCTTCTTGAGCAAGA
AATTCCTCAGAGTGTTCCTGGAGTACAGACTTTACTAGGAGCTGGAAGACAGTCCTTGCT
ACGAACAAATAAAAGCTGCAAGCATGTGGTATGGAAAGGATCTGCCCTGGCTGCACTGCA
CTGTGGGAGGCCGCCAGAGTCTCCAGTTAACTACGGTAGCCCACCTAGCATTGCGGATAC
TCTGTTTTCAAGGAAGCTGAATGGGAAATACAGACTTGAACGACTTGTTCCAACTGCAGT
TTATCAGCACATGAAGATGCATAAGCGAATTCTTGGACACTTATCATCGGTGTACTGTGT
AACTTTTGATCGAACTGGCAGGCGGATATTTACTGGTTCTGATGATTGTCTTGTGAAAAT
CTGGGCCACAGACGATGGAAGATTGCTAGCTACTTTAAGAGGACATGCTGCTGAAATATC
AGACATGGCTGTAAACTATGAGAATACTATGATAGCAGCTGGAAGTTGTGATAAAATGAT
TCGTGTCTGGTGTCTTCGAACCTGTGCACCTTTGGCTGTTCTTCAGGGACATAGTGCATC
TATTACATCACTACAGTTCTCACCATTGTGCAGTGGCTCAAAGAGATACCTGTCTTCTAC
AGGGGCGGACGGCACTATTTGCTTTTGGCTTTGGGATGCTGGAACCCTTAAAATAAATCC
AAGACCCACAAAATTTACAGAGCGTCCTCGGCCTGGAGTGCAAATGATATGTTCTTCGTT
CAGTGCTGGTGGGATGTTTTTGGCCACTGGAAGCACTGACCATATTATTAGAGTTTATTT
TTTTGGATCAGGTCAGCCAGAAAAAATATCAGAATTGGAGTTTCATACTGACAAAGTTGA
CAGTATCCAGTTTTCCAACACTAGTAACAGGTTTGTGAGTGGTAGTCGTGATGGGACAGC
ACGAATTTGGCAGTTTAAACGAAGGGAATGGAAAAGCATTTTGTTAGATATGGCTACTCG
TCCAGCAGGCCAAAATCTTCAAGGCATAGAAGACAAAATCACAAAAATGAAAGTAACTAT
GGTAGCTTGGGATCGACATGACAACACAGTTATAACTGCAGTTAATAACATGACTCTGAA
AGTTTGGAATTCTTATACTGGTCAACTGATACATGTTCTAATGGGTCATGAAGATGAGGT
GTTTGTTCTTGAGCCACACCCATTTGATCCTAGAGTTCTCTTCTCTGCTGGTCATGATGG
AAATGTGATAGTGTGGGATCTAGCAAGAGGAGTCAAAGTTCGATCTTATTTCAATATGAT
TGAAGGACAAGGACATGGTGCAGTGTTTGACTGCAAATGCTCCCCTGATGGTCAGCACTT
TGCATGTACAGACTCTCATGGACATCTTTTAATTTTTGGTTTTGGGTCCAGTAGCAAGTA
TGACAAGATAGCAGATCAGATGTTTTTTCACAGTGATTATCGGCCTCTTATCCGTGATGC
GAACAATTTTGTATTAGATGAGCAGACGCAGCAGGCACCTCACCTCATGCCTCCCCCTTT
TCTGGTTGATGTTGATGGAAATCCTCATCCATCAAGGTACCAGCGATTGGTTCCTGGTCG
GGAGAACTGCAGGGAGGAGCAGCTCATTCCTCAGATGGGAGTAACTTCTTCAGGATTGAA
CCAAGTTTTGAGCCAGCAAGCAAACCAGGATATTAGTCCTTTAGACAGCATGATTCAAAG
ACTGCAGCAGGAGCAGGACCTGAGGCGTTCGGGTGAAGCAGGTGTTAGTAATGCCAGCCG
TGTGAACAGAGGCTCAGTAAGTTCTACCTCCGAAGTTCATTCACCACCAAATATAGGATT
AAGGCGCAGTGGCCAAATCGAAGGTGTACGGCAGATGCACAGCAATGCTCCGAGAAGTGA
AATAGCCACAGAGCGAGATCTTGTTGCTTGGAGTCGGAGGGTAGTAGTGCCTGAGCTCTC
GGCTGGTGTGGCTAGTAGACAAGAAGAATGGAGAACTGCAAAGGGAGAAGAGGAAATAAA
GAGTTATAGATCAGAAGAGAAAAGGAAACACTTAACTGTTGCAAAAGAGAATAAAATACT
TACTGTCTCAAAGAATCATGCTCATGAGCATTTCCTGGATCTTGGGGATTCTAAAAAGCA
GCAAGCGAATCAGCACAATTACCGTACAAGATCTGCACTGGAAGAAACACCCAGGCCCTT
AGAGGAGCTAGAAAACGGAACTAGTTCTTCAGATGAAGGTGAAGTACTTGCTGTCAGTGG
TGGGACTTCTGAGGAAGAGGAGCGAGCATGGCACAGTGATGGCAGCTCCAGTGACTACTC
CAGTGATTATTCTGATTGGACAGCAGATGCTGGAATTAACTTGCAGCCACCAAAGAAAGT
TCCTAAGCATAAAACCAAGAAACCAGAAAGTAGTTCAGATGAAGAAGAAGAATCTGAAAA
CCAGAAGCAAAAACATATTAAAAAGGAAAGAAAAAAAGCAAATGAAGAAAAAGATGGACC
AACATCACCAAAGAAAAAAAAGCCCAAAGAAAGAAAACAAAAGAGATTGGCTGTAGGAGA
ACTAACTGAGAATGGCCTAACGTTAGAAGAGTGGTTGCCTTCAGCTTGGATTACAGACAC
ACTTCCCAGGAGATGTCCATTTGTGCCACAGATGGGTGATGAGGTTTATTATTTTCGACA
AGGGCATGAAGCATATGTTGAAATGGCCCGGAAAAATAAAATTTATAGTATCAATCCTAA
AAAGCAGCCATGGCATAAGATGGAACTAAGGGAACAAGAACTAATGAAAATTGTTGGTAT
AAAGTATGAAGTGGGGTTGCCTACCCTTTGCTGCCTTAAACTTGCTTTTCTAGATCCTGA
TACTGGCAAACTGACCGGTGGATCATTTACCATGAAATACCATGATATGCCTGACGTCAT
AGATTTTCTAGTCTTGAGACAACAATTTGATGATGCAAAGTATAGACGATGGAATATAGG
TGACCGCTTCAGATCTGTCATAGATGATGCCTGGTGGTTTGGAACAATTGAAAGTCAAGA
GCCTCTTCAACCTGAGTACCCTGATAGTTTGTTTCAGTGTTATAATGTATGTTGGGACAA
TGGAGATACAGAAAAGATGAGTCCTTGGGATATGGAATTAATACCTAATAATGCTGTCTT
TCCAGAAGAACTGGGTACCAGTGTTCCTTTAACTGATGTTGAATGTAGGTCGCTAATTTA
TAAACCTCTTGATGGAGAATGGGGAGCCAATCCCAGGGATGAAGAATGTGAAAGAATTGT
TGGAGGAATAAATCAGCTGATGACACTAGATATTGCGTCTGCATTTGTTGCCCCTGTGGA
CCTTCAAGCTTATCCCATGTATTGCACTGTGGTGGCCTATCCAACGGATCTAAGTACAAT
TAAACAAAGACTGGAGAACAGGTTTTACAGGCGCTTTTCATCACTAATGTGGGAAGTTCG
ATATATAGAACATAATACACGAACATTCAATGAGCCAGGAAGCCCAATTGTGAAATCTGC
TAAATTTGTGACTGATCTTCTCCTGCATTTTATAAAGGATCAGACTTGTTATAACATAAT
TCCACTTTACAACTCAATGAAGAAGAAAGTTTTGTCTGACTCTGAGGAAGAAGAGAAAGA
TGCTGATGTTCCAGGGACTTCTACCAGAAAGCGCAAGGATCATCAACCTAGAAGAAGGTT
ACGCAACAGAGCTCAGTCTTACGATATTCAGGCATGGAAGAAACAATGTCAAGAATTACT
GAATCTCATATTTCAATGTGAAGACTCAGAACCTTTTCGACAGCCAGTGGATCTTCTTGA
ATATCCAGACTACAGAGACATCATTGACACTCCAATGGACTTTGCCACTGTTAGAGAGAC
TTTAGAGGCTGGGAATTATGAGTCACCCATGGAGTTATGTAAAGATGTCAGGCTCATTTT
CAGTAATTCTAAAGCATACACACCAAGCAAGAGATCAAGGATTTACAGCATGAGTTTACG
CCTGTCTGCTTTCTTTGAAGAACATATTAGTTCAGTTTTGTCAGATTATAAATCTGCTCT
TCGTTTTCATAAAAGAAACACCATAAGCAAGAAGAGGAAGAAGCGAAACAGGAGCAGTTC
CCTGTCCAGCAGTGCTGCCTCAAGCCCTGAAAGGAAAAAAAGGATCTTAAAACCCCAGCT
AAAGTCAGAAGTATCTACCTCTCCATTCTCCATACCTACAAGATCAGTACTACCAAGACA
TAATGCTGCGCAAATGAATGGTAAACCAGAATCCAGTTCTGTGGTTCGAACTAGGAGCAA
CCGTGTAGCTGTAGATCCAGTTGTCACCGAGCAGCCCTCTACATCATCAGCCACAAAAGC
TTTTGTTTCAAAAACTAATACATCTGCCATGCCAGGAAAAGCAATGCTAGAGAATTCTGT
GAGACATTCCAAAGCCTTGAGCACACTTTCCAGCCCTGATCCGCTCACATTCAGCCATGC
TACAAAGAATAATTCTGCAAAAGAAAACATGGAAAAGGAAAAGCCTGTCAAACGTAAAAT
GAAGTCTTCTGTGTTTTCAAAAGCATCTCCACTTCCAAAGTCAGCCGCAGTCATAGAGCA
AGGAGAGTGTAAGAACAATGTTCTTATACCAGGAACCATTCAAGTAAATGGCCATGGAGG
ACAACCATCAAAACTCGTGAAGAGAGGACCTGGGAGGAAGCCCAAGGTAGAAGTTAACAC
CAGCAGTGGTGAAGTGACACACAAGAAAAGAGGTAGAAAGCCCAAGAATCTGCAGTGTGC
AAAGCAGGAAAACTCTGAGCAAAATAACATGCATCCCATCAGGGCTGACGTGCTTCCTTC
TTCAACATGCAACTTCCTTTCTGAAACTAATGCTGTCAAGGAGGATTTGTTACAGAAAAA
GAGTCGTGGAGGCAGAAAACCCAAAAGGAAGATGAAAACTCACAACCTAGATTCAGAACT
CATAGTTCCTACAAATGTTAAAGTGTTAAGGAGAAGTAACCGGAAAAAAACAGATGATCC
TATAGATGAGGAAGAGGAGTTTGAAGAACTCAAAGGCTCTGAGCCTCACATGAGAACTAG
AAATCAGGGTCGAAGGACAGCTTTCTATAATGAGGATGACTCGGAGGAAGAACAGAGACA
GCTGTTGTTCGAGGACACCTCCTTGACATTTGGAACTTCTAGTAGAGGACGAGTCCGAAA
GTTGACTGAAAAAGCAAAGGCTAATTTAATTGGTTGGTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC
Protein sequence predicted based on nucleotide sequence
MSRERKGLSELRSELYFLIARFLEDGPCQQAAQVLIREVAEKELLPRRTDWTGKEHPRTY QNLVKYYRHLAPDHLLQICHRLGPLLEQEIPQSVPGVQTLLGAGRQSLLRTNKSCKHVVW KGSALAALHCGRPPESPVNYGSPPSIADTLFSRKLNGKYRLERLVPTAVYQHMKMHKRIL GHLSSVYCVTFDRTGRRIFTGSDDCLVKIWATDDGRLLATLRGHAAEISDMAVNYENTMI AAGSCDKMIRVWCLRTCAPLAVLQGHSASITSLQFSPLCSGSKRYLSSTGADGTICFWLW DAGTLKINPRPTKFTERPRPGVQMICSSFSAGGMFLATGSTDHIIRVYFFGSGQPEKISE LEFHTDKVDSIQFSNTSNRFVSGSRDGTARIWQFKRREWKSILLDMATRPAGQNLQGIED KITKMKVTMVAWDRHDNTVITAVNNMTLKVWNSYTGQLIHVLMGHEDEVFVLEPHPFDPR VLFSAGHDGNVIVWDLARGVKVRSYFNMIEGQGHGAVFDCKCSPDGQHFACTDSHGHLLI FGFGSSSKYDKIADQMFFHSDYRPLIRDANNFVLDEQTQQAPHLMPPPFLVDVDGNPHPS RYQRLVPGRENCREEQLIPQMGVTSSGLNQVLSQQANQDISPLDSMIQRLQQEQDLRRSG EAGVSNASRVNRGSVSSTSEVHSPPNIGLRRSGQIEGVRQMHSNAPRSEIATERDLVAWS RRVVVPELSAGVASRQEEWRTAKGEEEIKSYRSEEKRKHLTVAKENKILTVSKNHAHEHF LDLGDSKKQQANQHNYRTRSALEETPRPLEELENGTSSSDEGEVLAVSGGTSEEEERAWH SDGSSSDYSSDYSDWTADAGINLQPPKKVPKHKTKKPESSSDEEEESENQKQKHIKKERK KANEEKDGPTSPKKKKPKERKQKRLAVGELTENGLTLEEWLPSAWITDTLPRRCPFVPQM GDEVYYFRQGHEAYVEMARKNKIYSINPKKQPWHKMELREQELMKIVGIKYEVGLPTLCC LKLAFLDPDTGKLTGGSFTMKYHDMPDVIDFLVLRQQFDDAKYRRWNIGDRFRSVIDDAW WFGTIESQEPLQPEYPDSLFQCYNVCWDNGDTEKMSPWDMELIPNNAVFPEELGTSVPLT DVECRSLIYKPLDGEWGANPRDEECERIVGGINQLMTLDIASAFVAPVDLQAYPMYCTVV AYPTDLSTIKQRLENRFYRRFSSLMWEVRYIEHNTRTFNEPGSPIVKSAKFVTDLLLHFI KDQTCYNIIPLYNSMKKKVLSDSEEEEKDADVPGTSTRKRKDHQPRRRLRNRAQSYDIQA WKKQCQELLNLIFQCEDSEPFRQPVDLLEYPDYRDIIDTPMDFATVRETLEAGNYESPME LCKDVRLIFSNSKAYTPSKRSRIYSMSLRLSAFFEEHISSVLSDYKSALRFHKRNTISKK RKKRNRSSSLSSSAASSPERKKRILKPQLKSEVSTSPFSIPTRSVLPRHNAAQMNGKPES SSVVRTRSNRVAVDPVVTEQPSTSSATKAFVSKTNTSAMPGKAMLENSVRHSKALSTLSS PDPLTFSHATKNNSAKENMEKEKPVKRKMKSSVFSKASPLPKSAAVIEQGECKNNVLIPG TIQVNGHGGQPSKLVKRGPGRKPKVEVNTSSGEVTHKKRGRKPKNLQCAKQENSEQNNMH PIRADVLPSSTCNFLSETNAVKEDLLQKKSRGGRKPKRKMKTHNLDSELIVPTNVKVLRR SNRKKTDDPIDEEEEFEELKGSEPHMRTRNQGRRTAFYNEDDSEEEQRQLLFEDTSLTFG TSSRGRVRKLTEKAKANLIGW*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
199
End on reference sequence
5664
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
