Gene:
Gene Symbol:
Flt3
Gene Name:
FMS-like tyrosine kinase 3
Original Clone ID:
FLH481707.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297388
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3003nts
Open reading frame: 35 to 3037
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGCGGGCGTTGGCGCAGCGCAGCGA
CCGGCGGCTGCTGCTGCTTGTTGTTTTGTCAGTAATGATTCTTGAGACCGTTACAAACCA
AGACCTGCCTGTGATCAAGTGTGTTTTAATCAGTCATGAGAACAATGGCTCATCAGCGGG
AAAGCCATCATCGTACCGAATGGTGCGAGGATCCCCAGAAGACCTCCAGTGTGCCCCGAG
GCGCCAGAGTGAAGGGACGGTATATGAAGCGGCCACCGTGGAGGTGGCCGAGTCTGGGTC
CATCACCCTGCAAGTGCAGCTCGCCACCCCAGGGGACCTTTCCTGCCTCTGGGTCTTTAA
GCACAGCTCCCTGGGCTGCCAGCCGCACTTTGATTTACAAAACAGAGGAATCGTTTCCAT
GGCCATCTTGAACGTGACAGAGACCCAGGCAGGAGAATACCTACTCCATATTCAGAGCGA
AGCCGCCAACTACACAGTACTGTTCACAGTGAATGTAAGAGATACACAGCTGTACGTGCT
AAGAAGACCTTACTTTAGGAAGATGGAAAACCAGGACGCACTGCTCTGCATCTCCGAGGG
TGTTCCAGAGCCCACTGTGGAGTGGGTGCTCTGCAGCTCCCACAGGGAAAGCTGTAAAGA
AGAAGGCCCTGCTGTTGTCAGAAAGGAGGAAAAGGTACTTCATGAGTTGTTCGGAACAGA
CATCAGATGCTGTGCTAGAAATGCACTGGGCCGCGAATGCACCAAGCTGTTCACCATAGA
TCTAAACCAGGCTCCTCAGAGCACACTGCCCCAGTTATTCCTGAAAGTGGGGGAACCCTT
GTGGATCAGGTGTAAGGCCATCCATGTGAACCATGGATTCGGGCTCACCTGGGAGCTGGA
AGACAAAGCCCTGGAGGAGGGCAGCTACTTTGAGATGAGTACCTACTCCACAAACAGGAC
CATGATTCGGATTCTCTTGGCCTTTGTGTCTTCCGTGGGAAGGAACGACACCGGATATTA
CACCTGCTCTTCCTCAAAGCACCCCAGCCAGTCAGCGTTGGTGACCATCCTAGAAAAAGG
GTTTATAAACGCTACCAGCTCGCAAGAAGAGTATGAAATTGACCCGTACGAAAAGTTCTG
CTTCTCAGTCAGGTTTAAAGCGTACCCACGAATCCGATGCACGTGGATCTTCTCTCAAGC
CTCATTTCCTTGTGAACAGAGAGGCCTGGAGGATGGGTACAGCATATCTAAATTTTGCGA
TCATAAGAACAAGCCAGGAGAGTACATATTCTATGCAGAAAATGATGACGCCCAGTTCAC
CAAAATGTTCACGCTGAATATAAGAAAGAAACCTCAAGTGCTAGCAAATGCCTCAGCCAG
CCAGGCGTCCTGTTCCTCTGATGGCTACCCGCTACCCTCTTGGACCTGGAAGAAGTGTTC
GGACAAATCTCCCAATTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAA
CAGAAAAGTGTTTGGCCAGTGGGTGTCGAGCAGTACTCTAAATATGAGTGAGGCCGGGAA
AGGGCTTCTGGTCAAATGCTGTGCGTACAATTCTATGGGCACGTCTTGCGAAACCATCTT
TTTAAACTCACCAGGCCCCTTCCCTTTCATCCAAGACAACATCTCCTTCTATGCGACCAT
TGGGCTCTGTCTCCCCTTCATTGTTGTTCTCATTGTGTTGATCTGCCACAAATACAAAAA
GCAATTTAGGTACGAGAGTCAGCTGCAGATGATCCAGGTGACTGGCCCCCTGGATAACGA
GTACTTCTACGTTGACTTCAGGGACTATGAATATGACCTTAAGTGGGAGTTCCCGAGAGA
GAACTTAGAGTTTGGGAAGGTCCTGGGGTCTGGCGCTTTCGGGAGGGTGATGAACGCCAC
GGCCTATGGCATTAGTAAAACGGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGA
GAAAGCTGACAGCTGTGAAAAAGAAGCTCTCATGTCGGAGCTCAAAATGATGACCCACCT
GGGACACCATGACAACATCGTGAATCTGCTGGGGGCATGCACACTGTCAGGGCCAGTGTA
CTTGATTTTTGAATATTGTTGCTATGGTGACCTCCTCAACTACCTAAGAAGTAAAAGAGA
GAAGTTTCACAGGACATGGACAGAGATTTTTAAGGAACATAATTTCAGTTTTTACCCTAC
TTTCCAGGCACATTCAAATTCCAGCATGCCTGGTTCACGAGAAGTTCAGTTACACCCGCC
CTTGGATCAGCTCTCAGGGTTCAATGGGAATTTAATTCATTCTGAAGATGAGATTGAATA
TGAAAACCAGAAGAGGCTGGCAGAAGAAGAGGAGGAAGATTTGAACGTGCTGACGTTTGA
AGACCTCCTTTGCTTTGCGTACCAAGTGGCCAAAGGCATGGAATTCCTGGAGTTCAAGTC
GTGTGTCCACAGAGACCTGGCAGCCAGGAATGTGTTGGTCACCCACGGGAAGGTGGTGAA
GATCTGTGACTTTGGACTGGCCCGAGACATCCTGAGCGACTCCAGCTACGTCGTCAGGGG
CAACGCACGGCTGCCGGTGAAGTGGATGGCACCTGAGAGCTTATTTGAAGGGATCTACAC
AATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCACTGGGTGT
GAACCCTTACCCTGGCATTCCTGTCGACGCTAACTTCTATAAACTGATTCAGAGTGGATT
TAAAATGGAGCAGCCATTCTATGCCACAGAAGGGATATACTTTGTAATGCAATCCTGCTG
GGCTTTTGACTCAAGGAAGCGGCCATCCTTCCCCAACCTGACTTCATTTTTAGGATGTCA
GCTGGCAGAGGCAGAAGAAGCGATGTATCAGAACATGGGTGGCAACGTCCCAGAACATCC
ATCCATCTACCAAAACAGGCGGCCCCTCAGCAGAGAGGCAGGCTCAGAGCCGCCATCGCC
ACAGGCCCAGGTGAAGATTCACGGAGAAAGAAGTTAGGGCCTCATGGGCCCAGCTTTCTT
GTAC
Protein sequence predicted based on nucleotide sequence
MRALAQRSDRRLLLLVVLSVMILETVTNQDLPVIKCVLISHENNGSSAGKPSSYRMVRGS PEDLQCAPRRQSEGTVYEAATVEVAESGSITLQVQLATPGDLSCLWVFKHSSLGCQPHFD LQNRGIVSMAILNVTETQAGEYLLHIQSEAANYTVLFTVNVRDTQLYVLRRPYFRKMENQ DALLCISEGVPEPTVEWVLCSSHRESCKEEGPAVVRKEEKVLHELFGTDIRCCARNALGR ECTKLFTIDLNQAPQSTLPQLFLKVGEPLWIRCKAIHVNHGFGLTWELEDKALEEGSYFE MSTYSTNRTMIRILLAFVSSVGRNDTGYYTCSSSKHPSQSALVTILEKGFINATSSQEEY EIDPYEKFCFSVRFKAYPRIRCTWIFSQASFPCEQRGLEDGYSISKFCDHKNKPGEYIFY AENDDAQFTKMFTLNIRKKPQVLANASASQASCSSDGYPLPSWTWKKCSDKSPNCTEEIP EGVWNKKANRKVFGQWVSSSTLNMSEAGKGLLVKCCAYNSMGTSCETIFLNSPGPFPFIQ DNISFYATIGLCLPFIVVLIVLICHKYKKQFRYESQLQMIQVTGPLDNEYFYVDFRDYEY DLKWEFPRENLEFGKVLGSGAFGRVMNATAYGISKTGVSIQVAVKMLKEKADSCEKEALM SELKMMTHLGHHDNIVNLLGACTLSGPVYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFK EHNFSFYPTFQAHSNSSMPGSREVQLHPPLDQLSGFNGNLIHSEDEIEYENQKRLAEEEE EDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHRDLAARNVLVTHGKVVKICDFGLARDIL SDSSYVVRGNARLPVKWMAPESLFEGIYTIKSDVWSYGILLWEIFSLGVNPYPGIPVDAN FYKLIQSGFKMEQPFYATEGIYFVMQSCWAFDSRKRPSFPNLTSFLGCQLAEAEEAMYQN MGGNVPEHPSIYQNRRPLSREAGSEPPSPQAQVKIHGERS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
225
End on reference sequence
3227
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
