Gene:
Gene Symbol:
Tpo
Gene Name:
thyroid peroxidase
Original Clone ID:
FLH481712.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297393
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2745nts
Open reading frame: 35 to 2779
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGAACACTTGGAGCTATGGCAAT
AATGCTGGTGGTTATGGGAACTGTAATTTTCCTCTCTTTTATCCTGAGAAGCAGAGACAT
CTTGTGTGGGAAGACCATGAAGTCCCATGTTATCAGTGCTGTGGAAACGAGCCAGCTCAT
GGTGGACCATGCAGTCTACAACACCATGAAAAGAAACCTCAAGAAAAGGGAAGTCCTTTC
TCCAGCCCAGCTTCTCTCTTTCTTTAAGCTGCCCGAGTCCACCAGTGGGGCTATTTCCCG
AGCAGCAGAGATTATGGAAACATCAATACAAGTCATGAAACGTGAACAGTCACAGTTCTC
CACGGATGCCTTATCAGCAGACATTCTGGGCACAATTGCCAACCTGTCAGGATGCTTGCC
TTTCATGCTGCCACCAAGATGTCCTGACACCTGCCTGGCAAATAAGTACCGGCCCATCAC
AGGGGCGTGCAACAATAGAGATCACCCCAGATGGGGAGCCTCCAACACAGCCCTAGCAAG
ATGGCTGCCTCCTGTCTATGAAGATGGCTTCAGTCAGCCCAAAGGCTGGAACCCTAATTT
CTTATACCACGGCTTCCCACTGCCCCCGGTACGGGAAGTGACAAGGCACCTCATTCAAGT
TTCCAATGAGGCTGTGACCGAAGATGACCAGTACTCTGATTTTCTGCCGGTGTGGGGACA
GTACATCGATCATGACATTGCTCTCACACCACAGAGCACTAGCACAGCAGCCTTCTGGGG
AGGTGTCGACTGCCAGCTGACCTGTGAGAACCAAAATCCTTGCTTCCCCATACAGCTTCC
CTCAAACTCCTCAGGGACCACTGCATGCCTGCCTTTCTACCGCTCCTCCGCCGCTTGTGG
CACTGGGGACCAAGGTGCTCTCTTTGGCAACCTGTCTGCAGCCAATCCGAGGCAGCAGAT
GAATGGCTTGACCTCCTTCCTTGATGCTTCCACTGTGTACGGCAGCTCCCCTGGCGTTGA
GAAGCAGCTGCGCAACTGGAGCAGCTCGGCAGGACTGCTGCGTGTCAACACTCTCCACCT
AGATGCTGGCCGTGCCTACCTGCCCTTCGCAACAGCCGCCTGCGCTCCAGAGCCTGGTAC
CCCACGCACCAACCGCACGCCCTGCTTCCTGGCTGGAGACGGTCGCGCCAGTGAGGTCCC
TGCCCTGGCAGCGGTACACACCTTGTGGCTGCGCGAGCACAACCGCCTGGCTTCGGCCTT
CAAGGCCATTAACAAGCACTGGAGCGCCAACACTGCCTACCAGGAGGCGCGCAAGGTGGT
AGGGGCACTGCACCAGATCATCACCATGAGGGATTATATCCCCAAAATCCTGGGTCCTGA
TGCCTTCAGGCAGTATGTGGGCCCCTATGAAGGCTACAACCCCACTGTGAACCCTACTGT
GTCCAACATCTTCTCCACTGCTGCCTTTCGCTTTGGCCATGCCACAGTCCATCCACTGGT
GAGACGGCTAAACACTGACTTCCAGGAGCACACAGAGCTCCCCAGGTTGCAGCTGCGTGA
TGTCTTCTTCAGACCCTGGAGGCTTATCCAGGAAGGTGGTTTGGATCCGATAGTGAGAGG
CCTCCTGGCGAGAGCAGCCAAGCTGCAAGTCCAAGGGCAGCTGATGAATGAGGAGCTGAC
CGAGAGGCTCTTCGTGCTGTCTAACGTGGGCACCTTGGATCTGGCATCACTGAACTTGCA
GAGGGGCCGGGATCATGGCTTACCAGACTACAATGAATGGAGAGAGTTCTGTGGCTTGTC
ACGCCTGGAGACACCAGCTGAGCTGAACAAGGCCATTGCCAACAGAAGCATGGTCAACAA
GATAATGGACTTATACAAGCATGCTGACAACATTGACGTCTGGTTGGGAGGCTTGGCTGA
AAAGTTCTTGCCGGGGGCCCGCACTGGTCCTCTGTTTGCATGTATCATTGGGAAGCAGAT
GAAGGCTCTGAGGGATGGGGACAGGTTTTGGTGGGAGAACACCAATGTCTTCACAGACGC
TCAGAGGCAGGAACTAGAAAAGCATTCACTACCTCGGGTCATCTGTGACAACACTGGACT
CACCAGAGTACCTGTGGATGCCTTCCGTATTGGAAAGTTCCCCCAAGACTTTGAATCCTG
TGAGGACATCCCGAGCATGGACCTTGAACTATGGAGGGAGACCTTCCCACAAGATGACAA
GTGTGTCTTCCCAGAGGAGGTGGACAATGGGAACTTTGTGCACTGTGAAGAGTCTGGGAA
GCTGGTGCTTGTATACTCCTGTTTCCATGGATACAAGCTGCAGGGCCAGGAGCAGGTCAC
ATGTACCCAGAAGGGATGGGACTCCGAACCTCCTGTCTGTAAAGATGTTAATGAGTGTGC
AGATCTGACACACCCACCTTGCCACCCCTCTGCACAGTGCAAGAACACCAAGGGAAGCTT
CCAGTGTGTGTGCACAGACCCCTATGTGCTAGGTGAGGATGAGAAGACCTGCATAGATTC
TGGCAGGCTACCTCGGGCATCCTGGGTCTCCATTGCACTGGGTGCACTTCTCATTGGTGG
TTTGGCCAGTCTCACCTGGATAGTAATTTGCAGGTGGACACATGCTGATAAGAAAGCCAC
ATTGCCGATCACAGAGAGAGTGACCACCCAGTCAGGATGCAGAAAGAGTCAGGGGAGGGG
GATTTCACCACACAAGGCCGCAGCTCAAGACACTGGACAGGAACCTGCAAGTGGATCCAG
GGTCCTCCTGTGCGAATAGGGCCTCATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MRTLGAMAIMLVVMGTVIFLSFILRSRDILCGKTMKSHVISAVETSQLMVDHAVYNTMKR NLKKREVLSPAQLLSFFKLPESTSGAISRAAEIMETSIQVMKREQSQFSTDALSADILGT IANLSGCLPFMLPPRCPDTCLANKYRPITGACNNRDHPRWGASNTALARWLPPVYEDGFS QPKGWNPNFLYHGFPLPPVREVTRHLIQVSNEAVTEDDQYSDFLPVWGQYIDHDIALTPQ STSTAAFWGGVDCQLTCENQNPCFPIQLPSNSSGTTACLPFYRSSAACGTGDQGALFGNL SAANPRQQMNGLTSFLDASTVYGSSPGVEKQLRNWSSSAGLLRVNTLHLDAGRAYLPFAT AACAPEPGTPRTNRTPCFLAGDGRASEVPALAAVHTLWLREHNRLASAFKAINKHWSANT AYQEARKVVGALHQIITMRDYIPKILGPDAFRQYVGPYEGYNPTVNPTVSNIFSTAAFRF GHATVHPLVRRLNTDFQEHTELPRLQLRDVFFRPWRLIQEGGLDPIVRGLLARAAKLQVQ GQLMNEELTERLFVLSNVGTLDLASLNLQRGRDHGLPDYNEWREFCGLSRLETPAELNKA IANRSMVNKIMDLYKHADNIDVWLGGLAEKFLPGARTGPLFACIIGKQMKALRDGDRFWW ENTNVFTDAQRQELEKHSLPRVICDNTGLTRVPVDAFRIGKFPQDFESCEDIPSMDLELW RETFPQDDKCVFPEEVDNGNFVHCEESGKLVLVYSCFHGYKLQGQEQVTCTQKGWDSEPP VCKDVNECADLTHPPCHPSAQCKNTKGSFQCVCTDPYVLGEDEKTCIDSGRLPRASWVSI ALGALLIGGLASLTWIVICRWTHADKKATLPITERVTTQSGCRKSQGRGISPHKAAAQDT GQEPASGSRVLLCE*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
End on reference sequence
2818
Start on reference sequence
74
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
