DNASU Plasmid | Zfp455 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Zfp455

Gene Name: zinc finger protein 455

Original Clone ID:

FLH481727.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00297408

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1386nts

Open reading frame: 35 to 1420

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGGAAATGTTGTCCTTCTGGGA
TGTGGCCATTGATTTCTCTCCAGAAGAGTGGGAATGCCTGGAGCCTGCTCAGTGGGATCT
GTACAGGGACGTGATGCTGGAGAATTTCAGCCACCTTGTGTTCCTGGGTCTTGCTGTCTC
TAAGCCATTCCTGGTGACATTTCTGGAGCAAAGACAAGGGCCTTGGGATATGAAGAGACA
AGCAGCAGCCACTGTGTACCCAGGAATAATACCTAATGATCCGAACAATTACAGCAAGTT
CACTAATTGTAAATCACTCCTTATTAAACGACGAAGAATTCATATAGGGGAGAAACCCTA
CAACTGTGGAGAATGTGGTAAGGCCCTTAGTTCTCATAAAACACTTTCTATACACCAGAG
ACTTCACACTGGAGACAAACCTTACAAGTGTGAAGAGTGTCATAAAGCCTTCAGTACTCG
CTCATCACTTTTTATACACATGAAAAATCATACTGATGAAAAAATCTACAAGTGTGAAGA
ATGTGGCAAAACATTTTACTATCCTTCAATGCTGAAGCAACATCAGAGAATTCATTCTGG
GGAGAAACCTTGCAAGTGTGAAGAATGTGGAAAATCCTTTGGCTTTCCCTCATTCCTTAA
GCAACATCAAAGAATTCATTGTAGAAAAAATGCCTACAAGGATGAAATATGTGTCAAAAG
ACTTTCCCCTCCTCTACCCCTTCAGGAACATGAGCAAATCCATACTGAAGAGAAACCCTA
CAAGTGTGGAGAATGTTATAAAGCCTTTCGTTATCACTCAGCCCTTAGGATACACAAGAC
AGTTCACACTGGAGAGAAACCTTACAAGTGTGAAGAGTGTAGCAGATGCTTTTCCTCATC
TTCCTGCCTTAAAAGACATCAAATACTTCACTCTGAAGACAACCCATACAAGTGTGAAGA
GTGTGGCAGATGTTTCTGCTCATCTTCATCTCTTAGACGACATCAAACATTTCATTCTGA
AGACAATCCCTATAAATGTGAAGAATGTGACAAAAGGTTTTCATGTTCTGCAAGTCTTCA
GGAGCATCAAACAATTCATACTGGAGAGAAACCATACACGTGTGAAAATTGTCACAAAGC
CTTTCGTTATCACTCATCCCTTAGGAGACATAAGACAGTTCATACCAGAGAGAAATCCTA
CAAGTGTGAAAAGTGTCCTAGGGGCTTTTCCTCATCCTCCTGCCTTGTAAGGCATCAAGC
AATTCACTCTGAAGACAATCCACACAAGTGTGTGGAATGTGGCAAAGCCTTTGTTAATGT
TTATAGCCTGACTCGACACATGACAGTTCATGCTGGAGAGAAATCCTACAAAGGTCTTAA
AACACTTATACTTCTTCAGTCCTTAAAAGACATCAACTAGGGCCTCATGGGCCCAGCTTT
CTTGTAC

Protein sequence predicted based on nucleotide sequence

MEEMLSFWDVAIDFSPEEWECLEPAQWDLYRDVMLENFSHLVFLGLAVSKPFLVTFLEQR
QGPWDMKRQAAATVYPGIIPNDPNNYSKFTNCKSLLIKRRRIHIGEKPYNCGECGKALSS
HKTLSIHQRLHTGDKPYKCEECHKAFSTRSSLFIHMKNHTDEKIYKCEECGKTFYYPSML
KQHQRIHSGEKPCKCEECGKSFGFPSFLKQHQRIHCRKNAYKDEICVKRLSPPLPLQEHE
QIHTEEKPYKCGECYKAFRYHSALRIHKTVHTGEKPYKCEECSRCFSSSSCLKRHQILHS
EDNPYKCEECGRCFCSSSSLRRHQTFHSEDNPYKCEECDKRFSCSASLQEHQTIHTGEKP
YTCENCHKAFRYHSSLRRHKTVHTREKSYKCEKCPRGFSSSSCLVRHQAIHSEDNPHKCV
ECGKAFVNVYSLTRHMTVHAGEKSYKGLKTLILLQSLKDIN*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1469

Start on reference sequence 84

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Zfp455

Symbol Nomenclature :

Zfp455

SYNONYM :

3732412P20Rik; Rslcan-10

Full Nomenclature :

zinc finger protein 455

GENEID :

218311

GI :

182888024

GenBank Accession :

BC160312

Mouse Genome Informatics :

3040708

Vega :

OTTMUSG00000029819

Target GenBank:

NM_001048204.1

Protein

SMART domain :

KRAB : krueppel associated box

SMART domain :

ZnF_C2H2 : zinc finger

UniProt :

Q7M6X9

Clone

Cloning Information :

481727

HIP Master Clone ID :

399455

IMAGE ID :

100064056

Original Clone ID :

FLH481727.01X

Organism

Species Specific ID:

218311

Genome

Chromosome :

Map Location :

13 B3|13 34.54 cM

Ensembl :

ENSMUSG00000051037

Reagents

Labome :

Zfp455-siRNA