DNASU Plasmid | Ptpre (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Ptpre

Gene Name: protein tyrosine phosphatase, receptor type, E

Original Clone ID:

FLH481760.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00297441

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2100nts

Open reading frame: 35 to 2134

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGCCCTTCTGTCCACTCCTGCT
GGCAAGTTTTAGCTTGTCGCTCGCCAGAGCTGGCCAGGGCAACGACACCACCCCAACAGA
GAGCAACTGGACCAGCACAACTGCAGGCCCTCCGGACCCTGGTGCATCCCAGCCGCTGCT
CACCTGGCTGCTGCTGCCCCTGCTCCTCCTCCTGTTCCTGCTTGCAGCCTACTTCTTCAG
GTTCCGGAAGCAGAGGAAGGCCGTGGTCAGCAGCAACGACAAGAAAATGCCTAACGGGAT
CTTAGAAGAGCAAGAGCAGCAGAGAGTGATGCTGCTGAGCAGATCTCCATCAGGCCCCAA
GAAGTTCTTCCCCATCCCCGTGGAGCACCTGGAGGAGGAGATCCGGGTGAGATCTGCGGA
TGACTGCAAGCGATTCCGAGAGGAGTTCAATTCATTGCCATCTGGACACATACAAGGAAC
CTTTGAACTAGCAAATAAAGAAGAAAACAGAGAAAAAAACAGATACCCCAACATTCTGCC
CAATGATCATTGCAGAGTGATTTTGAGCCAAGTGGATGGAATCCCCTGCTCTGACTACAT
TAATGCTTCCTACATCGATGGCTACAAAGAAAAGAACAAATTCATAGCAGCTCAAGGCCC
TAAGCAGGAGACAGTGAATGACTTCTGGAGAATGGTCTGGGAGCAAAGGTCAGCCACCAT
CGTCATGTTGACGAACCTGAAGGAGAGGAAGGAGGAGAAGTGCTACCAGTACTGGCCAGA
CCAGGGCTGTTGGACCTACGGCAACATCCGGGTGTGTGTAGAGGACTGCGTGGTCCTGGT
GGATTACACGATCCGAAAGTTCTGCATCCATCCGCAACTCCCAGACAGCTGCAAAGCCCC
GCGGCTGGTCTCACAGCTGCACTTCACCAGCTGGCCTGACTTCGGGGTGCCGTTTACCCC
CATCGGGATGCTCAAGTTCCTGAAGAAAGTGAAGACACTCAACCCCTCACATGCTGGGCC
CATTGTGGTTCACTGTAGCGCGGGCGTGGGTCGGACTGGCACCTTCATTGTGATCGATGC
CATGATGGACATGATACACTCGGAGCAGAAGGTTGACGTCTTTGAGTTTGTGTCTAGAAT
CCGCAATCAGCGCCCTCAGATGGTCCAGACGGATGTTCAGTATACATTCATCTACCAAGC
CTTACTGGAATACTACCTCTATGGGGACACAGAGCTGGATGTGTCCTCCCTGGAGAGGCA
CCTGCAGACGCTCCATAGCACAGCCACCCATTTTGACAAGATCGGGCTGGAGGAAGAGTT
CAGGAAGCTGACCAACGTGCGAATCATGAAGGAGAACATGAGGACGGGCAACCTGCCTGC
CAACATGAAGAAGGCCCGCGTCATCCAGATCATTCCATATGACTTCAATCGGGTCATCCT
GTCCATGAAAAGAGGGCAAGAGTTCACAGACTATATCAACGCATCCTTCATAGATGGCTA
CAGGCAGAAGGACTACTTCATGGCCACACAGGGGCCTCTGGCTCACACAGTTGAGGACTT
CTGGAGGATGGTATGGGAGTGGAAGTCTCACACAATCGTCATGCTGACGGAGGTGCAGGA
GCGGGAACAGGATAAATGCTACCAGTATTGGCCAACGGAGGGCTCGGTGACTCATGGAGA
TATAACTATAGAGATAAAGAGCGACACCCTGTCTGAAGCAATCAGCGTACGAGACTTTCT
GGTTACCTTCAAACAGCCCCTGGCCCGCCAGGAAGAGCAGGTCCGCATGGTGAGACAATT
CCATTTCCATGGCTGGCCTGAGGTTGGCATCCCCGCTGAAGGCAAAGGCATGATTGACCT
GATTGCAGCAGTGCAGAAGCAGCAGCAGCAGACGGGCAACCACCCCATCACCGTGCACTG
CAGCGCGGGAGCAGGGCGGACAGGTACATTCATAGCACTCAGTAACATTTTGGAACGAGT
GAAAGCCGAGGGACTCCTAGACGTGTTTCAAGCTGTGAAGAGCTTAAGACTTCAGAGACC
ACACATGGTGCAGACCCTGGAGCAATATGAATTCTGCTACAAAGTGGTACAAGATTTTAT
CGATATATTTTCTGATTATGCTAATTTCAAATAGGGCCTCATGGGCCCAGCTTTCTTGTA
C

Protein sequence predicted based on nucleotide sequence

MEPFCPLLLASFSLSLARAGQGNDTTPTESNWTSTTAGPPDPGASQPLLTWLLLPLLLLL
FLLAAYFFRFRKQRKAVVSSNDKKMPNGILEEQEQQRVMLLSRSPSGPKKFFPIPVEHLE
EEIRVRSADDCKRFREEFNSLPSGHIQGTFELANKEENREKNRYPNILPNDHCRVILSQV
DGIPCSDYINASYIDGYKEKNKFIAAQGPKQETVNDFWRMVWEQRSATIVMLTNLKERKE
EKCYQYWPDQGCWTYGNIRVCVEDCVVLVDYTIRKFCIHPQLPDSCKAPRLVSQLHFTSW
PDFGVPFTPIGMLKFLKKVKTLNPSHAGPIVVHCSAGVGRTGTFIVIDAMMDMIHSEQKV
DVFEFVSRIRNQRPQMVQTDVQYTFIYQALLEYYLYGDTELDVSSLERHLQTLHSTATHF
DKIGLEEEFRKLTNVRIMKENMRTGNLPANMKKARVIQIIPYDFNRVILSMKRGQEFTDY
INASFIDGYRQKDYFMATQGPLAHTVEDFWRMVWEWKSHTIVMLTEVQEREQDKCYQYWP
TEGSVTHGDITIEIKSDTLSEAISVRDFLVTFKQPLARQEEQVRMVRQFHFHGWPEVGIP
AEGKGMIDLIAAVQKQQQQTGNHPITVHCSAGAGRTGTFIALSNILERVKAEGLLDVFQA
VKSLRLQRPHMVQTLEQYEFCYKVVQDFIDIFSDYANFK*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 2423

Start on reference sequence 324

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Ptpre

Symbol Nomenclature :

Ptpre

SYNONYM :

PTPe; PTPepsilon; RPTPepsilon

Designation :

R-PTP-epsilon|protein-tyrosine phosphatase epsilon|receptor-type tyrosine-protein phosphatase epsilon

Full Nomenclature :

protein tyrosine phosphatase, receptor type, E

GENEID :

19267

GI :

182888054

GenBank Accession :

BC160345

Mouse Genome Informatics :

97813

Target GenBank:

NM_011212.3

Protein

SMART domain :

PTPc : Protein tyrosine phosphatase, catalytic domain

SMART domain :

PTPc_motif : Protein tyrosine phosphatase, catalytic domain motif

UniProt :

P49446

Clone

Cloning Information :

481760

HIP Master Clone ID :

399488

IMAGE ID :

100064089

Original Clone ID :

FLH481760.01X

Organism

Species Specific ID:

19267

Genome

Chromosome :

Map Location :

7 F3|7 81.27 cM

Ensembl :

ENSMUSG00000041836

Reagents

Labome :

Ptpre-antibody