DNASU Plasmid | Olfm4 (Mus musculus) in pENTR223.1 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: Olfm4

Gene Name: olfactomedin 4

Original Clone ID:

FLH481771.01X

Keyword:

None

Species:

Mus musculus

Type:

cDNA

Vector Name:

pENTR223.1

Format:

CLOSED

Source:

The ORFeome Collaboration

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

The ORFeome Collaboration

MmCD00297452

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 1617nts

Open reading frame: 35 to 1651

GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGAGTTACAGCCTTCTCTTTCTCCT
GGCCCTTCAGTTCTGCCTTGGCTCTGCCTCCCGGACAACTCTGACCTCTGCACATTCCCG
GGAATTGACCACACCTCCAACATCACCCCAGGCTACAGCTGCCTGGTTGCCTCCGGGAGG
CACTTCTTGGGCAGAAGGTGGGACTGTGTCTCAGCCACTTTCCAATTTCACTGGGTCTGT
GGACAGCCATGGGACCTGCCAGTGTTCTGTTTCCCTGCCGGATACCGCCTTTCCCGCTGA
CAGAGTGGAGCGCTTAGAGTACACAGCTCACATCCTTTCTCAGAAATTCGAGAGAGAGTT
TTCTAAGGTGAAGGAGTATGTCCAGCTAATAAGTGTGTATGAGAAGAGGCTCCTGAACCT
GACGGTCCGAGTAGAGGTCATGGAGAAGGACAGCATCTCTTACACAGAACTGGACTTTGA
GTTGATCAAGCTGGAAGTGAAGGAGATGCAAAAACTGGTCTTACAGCTGAAGAAGAATTT
TGTTGGAAGTACACATATTATTGACATGCTCGAAGTGGAGATAAGGAATATGACCCTCTT
GGTAGAGAAGCTGGAGTCTCTAGACCAAAACAATGTCCTTAGCATTCGCCGCCAGATCTT
GGCTCTGAAGACCAAGCTGAAAGAATGTGAGGCCTCCAAAAGTGACCTTGTGCCTGCCAC
ACCCCCTCCTCCTGCTCCTGGAAAGCTGTTAGGAGCAGCTGGAAGTATCCTCTGGATCCT
GAACTTTTGGTGCTTTTCTCTTTTCCACCAGCACACGCAAAATGTTTCATTGGCCCTTGG
AAGCTGTAGTCATGGTGGCGTGGTGAACATCAGCGCTCCTTCTGTGATTCAGCTCAACTG
GCTGGGGTTTAGTTATAAATATGGTGCCTGGGGCCGAGATTACTCTCCTCAGCATCCAGA
GAGAACCCTGTACTGGGTGGCACCTTTGAATACAGATGCAAGGGCTCTAGAGTATTACAG
ACTTTACGACTCATTGGACAATTTGTTAATCTATTCCCACTTCCGAGACTATCGGATTCG
CTATGGCCAAGGAGGTGGTACAGTAGCATTCAACAACAACCTGTATGTGAATTGGTACAA
TGGGGGGAACATTGCCAAAATTAATCTAACTACCAATGTAGTTGATGTGAATCGGCCCCT
CCCTCTGGCTGCCTACAATAATCGCTTCTCATATGCTAATGTGAATTGGCAAGACATTGA
CCTTGCTGTGGATGAGCAAGCACTGTGGGCAATTTATGCAACTGAGGCCAGCACTGGTAA
CATAGTGATTAGTAAACTCAATGACACCACTCTTGAGGTGATAAGCACTTGGGTTACCAA
GCAGTACAAGCCATCTGTTTCGAATGCCTTCATGGTATGTGGAGTTCTTTATGCTACTCG
CACTTTGAACACCAAAACAGAAGAGATCTTTTACTATTATGACACAAACACAGAGAGGGA
AGGCAACCTAGGCATCACAATGAGAAAGATGCAGGAAAGAATTCAGAGCATCAATTACCA
TCCCTTTGACCAAAAACTTTATGTCTATAATGATGGTTATCTTCTGAACTATGATCTTGT
CTTCTTACAGACGCCCAGGCAACCTGTCTAGGGCCTCATGGGCCCAGCTTTCTTGTAC

Protein sequence predicted based on nucleotide sequence

MSYSLLFLLALQFCLGSASRTTLTSAHSRELTTPPTSPQATAAWLPPGGTSWAEGGTVSQ
PLSNFTGSVDSHGTCQCSVSLPDTAFPADRVERLEYTAHILSQKFEREFSKVKEYVQLIS
VYEKRLLNLTVRVEVMEKDSISYTELDFELIKLEVKEMQKLVLQLKKNFVGSTHIIDMLE
VEIRNMTLLVEKLESLDQNNVLSIRRQILALKTKLKECEASKSDLVPATPPPPAPGKLLG
AAGSILWILNFWCFSLFHQHTQNVSLALGSCSHGGVVNISAPSVIQLNWLGFSYKYGAWG
RDYSPQHPERTLYWVAPLNTDARALEYYRLYDSLDNLLIYSHFRDYRIRYGQGGGTVAFN
NNLYVNWYNGGNIAKINLTTNVVDVNRPLPLAAYNNRFSYANVNWQDIDLAVDEQALWAI
YATEASTGNIVISKLNDTTLEVISTWVTKQYKPSVSNAFMVCGVLYATRTLNTKTEEIFY
YYDTNTEREGNLGITMRKMQERIQSINYHPFDQKLYVYNDGYLLNYDLVFLQTPRQPV*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1640

Start on reference sequence 24

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: spectinomycin

Bacterial Selection Condition: 100 ug/mL spectinomycin

Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.

Comments: None

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pENTR223.1

Synonyms:

pDONR-223.1, pENTR223-star-Sfi

Sequencing Primer:

Forward: M13F

Reverse: M13R

Description:

Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.

Comments:

Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php

Size (bp):

4311

Parent Vector:

None

Properties:

Gateway, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATACGCGTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGTACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAGCCAGGACAGAAATGCCTCGACTTCGCTGCTGCCCAAGGTTGCCGGGTGACGCACACCGTGGAAACGGATGAAGGCACGAACCCAGTGGACATAAGCCTGTTCGGTTCGTAAGCTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAACCCTCGAGCCACCCATGACCAAAATCCCTTAACGTGAGTTACGCGTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCG

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (Sfi sites flank the cassette present only in no insert form)

1265

1277

bacterial origin

ori

bacterial origin of replication

534

negative selection marker

ccdB

ccdB (no insert form only; lost in with insert form)

1312

1617

primer site

M13 F

M13 forward primer for insert sequencing

1113

1129

primer site

T7 Forward primer for insert sequencing

2902

2921

primer site

M13 reverse

M13 reverse primer for insert sequencing

2926

2942

recombination site

attL1

attL1 recombination site

1165

1264

recombination site

attL2

attL2 recombination site

2786

2885

selectable marker

CmR

chlR (no insert form only; lost in with insert form)

1959

2618

selectable marker

SpnR

spectinomycin resistance gene

3154

4164

Gene

Gene Symbol :

Olfm4

Symbol Nomenclature :

Olfm4

SYNONYM :

GC1; GW112; Gm296; Gm913; OlfD; pPD4

Designation :

OLM4|PU.1 difference product 4|olfactomedin-4

Full Nomenclature :

olfactomedin 4

GENEID :

380924

GI :

182888062

GenBank Accession :

BC160356

Mouse Genome Informatics :

2685142

Target GenBank:

NM_001030294.1

Protein

SMART domain :

OLF : Olfactomedin-like domains

UniProt :

D3YYD0

Clone

Cloning Information :

481771

HIP Master Clone ID :

399499

IMAGE ID :

100064100

Original Clone ID :

FLH481771.01X

Organism

Species Specific ID:

380924

Genome

Chromosome :

Map Location :

14 D3|14

Ensembl :

ENSMUSG00000022026

Reagents

Labome :

Olfm4-antibody