Gene:
Gene Symbol:
Enpp1
Gene Name:
ectonucleotide pyrophosphatase/phosphodiesterase 1
Original Clone ID:
FLH481786.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297467
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2718nts
Open reading frame: 35 to 2752
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGAGCGCGACGGCGACCAGGCAGG
GCACGGGCCCCGGCACGGGTCAGCGGGAAACGGTCGCGAGCTCGAGTCACCAGCCGCGGC
TTCGCTGCTCGCGCCCATGGACCTAGGAGAGGAGCCGCTGGAGAAGGCGGAGCGTGCCCG
CCCTGCCAAAGACCCCAACACCTACAAAGTGCTGTCGCTGGTTTTGTCAGTATGTGTGCT
AACAACAATTCTTGGTTGTATATTTGGGTTGAAACCAAGCTGCGCCAAAGAAGTAAAAAG
TTGCAAAGGCCGCTGCTTTGAAAGGACGTTCAGCAACTGTCGCTGTGATGCTGCCTGTGT
CAGCCTTGGGAACTGCTGTCTGGATTTCCAGGAGACCTGTGTGGAACCAACACATATATG
GACTTGCAACAAATTCAGGTGCGGTGAGAAGAGGCTGTCCAGGTTCGTGTGCTCCTGTGC
AGACGACTGCAAAACCCACAATGACTGTTGCATCAACTACAGTTCTGTGTGCCAAGATAA
GAAGAGTTGGGTAGAAGAAACATGTGAAAGCATCGATACCCCGGAGTGTCCAGCAGAGTT
TGAATCACCCCCTACTCTCTTGTTTTCTTTGGATGGATTCAGAGCTGAGTATTTGCACAC
CTGGGGTGGACTTCTTCCTGTCATTAGCAAGCTGAAAAACTGTGGAACATACACTAAAAA
CATGAGGCCTATGTACCCTACCAAGACGTTTCCCAATCATTACAGCATCGTCACAGGACT
TTATCCGGAATCCCATGGCATAATTGATAACAAGATGTATGATCCCAAAATGAATGCTTC
TTTCTCGCTTAAAAGTAAAGAGAAATTCAACCCTTTGTGGTACAAAGGACAGCCGATTTG
GGTGACCGCTAATCATCAGGAGGTCAAGTCCGGCACATACTTTTGGCCAGGATCAGATGT
GGAGATTGACGGGATTCTACCAGATATCTACAAAGTGTATAATGGGTCAGTACCATTTGA
AGAAAGGATTTTAGCTGTTCTTGAGTGGCTGCAGCTTCCTAGCCATGAAAGACCACACTT
TTACACTCTGTATTTAGAAGAACCAGATTCTTCAGGGCATTCACATGGACCAGTCAGCAG
CGAGGTCATCAAGGCCTTGCAGAAGGTTGACCGCCTGGTCGGCATGCTGATGGACGGCCT
GAAGGACCTGGGCTTGGATAAATGCCTGAACCTCATCCTCATTTCAGATCACGGCATGGA
ACAAGGCAGCTGTAAGAAGTATGTGTACCTGAATAAGTACTTGGGGGATGTGAACAATGT
GAAAGTTGTGTATGGACCTGCTGCTCGGTTGAGACCCACTGATGTTCCAGAGACATACTA
TTCATTTAACTATGAAGCCCTTGCAAAAAATCTTTCTTGCCGGGAGCCAAACCAGCATTT
CCGGCCTTACCTGAAACCCTTCTTACCCAAGCGCTTACACTTCGCTAAAAGTGACCGGAT
TGAGCCCCTGACCTTCTACCTGGACCCTCAGTGGCAACTTGCGTTGAATCCATCAGAGAG
GAAATATTGTGGAAGTGGATTTCATGGCTCTGACAACTTGTTTTCAAACATGCAAGCTCT
TTTTATTGGCTATGGACCTGCCTTCAAGCATGGTGCTGAAGTTGACTCCTTTGAAAATAT
TGAAGTCTATAACTTAATGTGTGACTTACTGGGTTTGATCCCAGCTCCCAATAATGGAAG
TCATGGCAGCCTTAACCACCTTCTAAAGAAACCCATTTACAACCCAAGTCATCCCAAAGA
AGAGGGCTTCCTGTCTCAGTGTCCAATCAAATCAACATCCAATGACCTCGGCTGCACATG
TGACCCTTGGATTGTGCCAATCAAGGACTTTGAGAAACAGCTTAATCTGACCACAGAAGA
TGATGACATTTACCATATGACTGTACCCTACGGGAGGCCCCGGATTCTACTGAAGCAGCA
CCGCGTCTGTCTACTCCAGCAGCAACAGTTCTTGACCGGATACAGCCTGGACCTCCTCAT
GCCCCTCTGGGCGTCTTACACCTTCCTCAGTAATGACCAATTCTCCAGAGATGACTTTTC
CAACTGTCTGTACCAGGATCTTCGGATTCCCCTTAGTCCTGTGCATAAATGTTCCTATTA
CAAAAGCAATTCTAAGCTAAGTTATGGGTTCCTCACGCCACCGAGACTAAATAGAGTTTC
AAATCACATATACTCTGAAGCGCTGCTTACATCTAATATAGTGCCAATGTATCAGAGTTT
TCAAGTTATATGGCACTACCTTCATGACACCCTACTGCAAAGGTATGCCCATGAAAGGAA
CGGCATCAATGTTGTCAGCGGTCCCGTGTTTGACTTTGATTATGACGGACGCTATGATTC
CTTAGAGATCCTGAAACAAAACAGCAGAGTCATCCGAAGCCAGGAAATTCTGATTCCGAC
TCACTTCTTCATTGTGCTCACCAGCTGCAAGCAGCTGTCTGAGACTCCCTTGGAGTGCTC
CGCCTTAGAGTCCTCAGCTTACATACTGCCTCATAGGCCTGATAACATTGAGAGCTGTAC
GCATGGGAAGCGGGAGTCTTCATGGGTCGAGGAGTTGCTGACCTTGCACAGAGCTCGGGT
CACAGACGTGGAACTCATCACTGGCCTCAGCTTCTACCAGGACCGACAAGAGTCAGTTTC
AGAACTGCTGAGGTTGAAAACACATTTGCCAATCTTCAGCCAAGAAGACTAGGGCCTCAT
GGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVL SLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQE TCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESI DTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFP NHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSG TYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSS GHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLN KYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKR LHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHG AEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKS TSNDLGCTCDPWIVPIKDFEKQLNLTTEDDDIYHMTVPYGRPRILLKQHRVCLLQQQQFL TGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFL TPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFD FDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPH RPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPI FSQED*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
112
End on reference sequence
2829
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
