Gene:
Gene Symbol:
Rims1
Gene Name:
regulating synaptic membrane exocytosis 1
Original Clone ID:
FLH481793.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297474
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4392nts
Open reading frame: 35 to 4426
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGTCCTCGGCCGTGGGGCCCCGAGG
TCCTCGCCCACCCACGGTGCCTCCCCCTATGCAAGAACTGCCCGACCTGAGCCACCTGAC
CGAGGAGGAGAGGAACATTATCATGGCAGTGATGGACCGGCAGAAGGAAGAGGAGGAAAA
AGAAGAGGCCATGCTCAAGTGTGTTGTCAGGGACATGGCGAAGCCTGCTGCCTGCAAAAC
ACCAAGAAATGCTGAAAGCCAGCCCCATCAACCACCACTGAACATTTTCAGATGTGTCTG
TGTTCCCAGAAAGCCAAGCAGCGAAGAGGGAGGCCCAGACAGAAACTGGAGATTGCATCA
ACAGTTTGAAAGCTACAAGGAACAAGTGAGAAAAATCGGAGAGGAAGCCAGGCGTTACCA
GGGCGAGCACAAAGATGATGCCCCGACGTGTGGAATCTGTCATAAGACAAAGTTTGCTGA
TGGATGTGGCCATCTCTGCTCCTATTGTCGCACCAAGTTCTGTGCACGCTGTGGAGGCCG
AGTGTCTCTGCGATCGAACAATGAGGACAAAGTGGTTATGTGGGTATGCAATTTATGTCG
AAAGCAACAAGAGATATTAACGAAATCTGGAGCGTGGTTCTTCGGAAGTGGTCCTCAGCA
GCCCAGTCAAGATGGGACTCTGAGTGACACGGCTACAGGTGCTGGATCTGAGGTGCCAAG
AGAAAAGAAAGCTCGGCTCCAAGAGCGATCAAGGTCTCAGACGCCCTTGAGTACAGCAGC
TGTCTCTTCCCAAGACACTGCTTCCCATGGTGCACCACTGGACAGGAACAAAGGGGCGGA
GCCCTCACAGCAAGCCTTGGGTCCTGAACAGAAGCAGGCATCAAGGTCAAGAAGTGAGCC
ACCAAGGGAAAGGAAGAAGGCTCCAGGGCTTTCAGAGCAGAATGGCAAGGGAGGCCAGAA
GAGTGAGCGCAAACGTGTCCCCAAGTCTGTGGTGCAACCCGGGGAAGGGACCGCGGATGA
ACGGGAGAGGAAGGAGAGGCGGGAAACCCGCAGGCTGGAGAAAGGGCGCTCTCAGGACTA
CCCGGACCGGCTCGAGAAACGCGAGGATGGCAGGGTGGCTGAAGACGAAAAGCAGAGGAA
GGAGGAGGAGGGCGTGTCCACGCCGGAGTACACGAGCTGCGAGGACGTGGAGCTGGAGAG
CGAGAGCGTGAGCGAGAAAGGTGACTTGGACTACTGGTTGGATCCTGCCACGTGGCATAG
CAGGGAAACGTCGCCTATTAGTTCGCATCCTGTAACGTGGCAGCCATCTAAAGAGGGGGA
CCGATTAATCGGCCGTGTTATTCTTAACAAAAGAACAACCATGCCCAAAGAATCAGGTGC
ATTATTGGGTCTCAAGGTGGTTGGAGGAAAAATGACGGACTTAGGGCGTCTTGGTGCTTT
CATCACCAAAGTAAAGAAGGGTAGCCTGGCAGATGTTGTCGGACACCTAAGAGCAGGGGA
TGAAGTTCTAGAGTGGAATGGTAAACCCCTGCCGGGAGCAACAAACGAAGAAGTTTACAA
CATTATTTTAGAATCAAAATCAGAACCTCAAGTTGAAATTATTGTTTCAAGGCCTATTGG
TGACATCCCCAGGATCCCTGAGAGTTCCCACCCTCCTCTGGAGTCCAGTTCAAGTTCCTT
TGAATCTCAGAAGATGGAAAGGCCTTCCATTTCTGTTATTTCTCCAACCAGCCCTGGAGC
TCTGAAAGATGCCCCACAAGTCTTACCAGGGCAACTCTCAGTGAAGCTGTGGTATGATAA
GGTGGGGCACCAGCTGATTGTAAATGTTCTACAAGCAACAGATCTACCCCCTAGAGTAGA
TGGGCGTCCCAGAAATCCCTATGTAAAAATGTATTTTCTTCCAGATAGAAGCGACAAGAG
TAAAAGAAGAACCAAAACGGTAAAGAAACTTCTAGAGCCAAAATGGAATCAAACATTTGT
CTACTCACACGTACATCGTCGAGATTTTCGAGAGCGAATGTTAGAAATTACTGTGTGGGA
CCAGCCAAGAGTACAGGACGAGGAGAGTGAATTTCTAGGAGAGATCCTCATAGAATTGGA
AACAGCACTTTTAGATGATGAGCCCCATTGGTATAAACTGCAGACACATGATGAATCTTC
ACTACCTCTGCCTCAGCCATCACCGTTCATGCCCAGGCGGCATATCCACGGAGAAAGCTC
CAGCAAAAAGCTACAAAGATCTCAGCGAATCAGTGATAGTGACATCTCAGATTATGAGGT
TGATGATGGTATTGGCGTAGTGCCTCCAGTGGGTTATAGAGCTAGTGCTAGAGAGAGTAA
AGCCACCACGTTAACAGTGCCAGAGCAGCAAAGAACCACACATCACCGCTCACGTTCCGT
GTCTCCTCATCGCGGCGATGATCAGGGAAGGCCTCGTTCACGTTTACCAAATGTGCCATT
ACAGAGGAGCTTAGATGAAATCCATCCAACACGAAGGTCACGTTCTCCAACCCGACACCA
TGATGCCTCCCGAAGCCTGGCCGATCACAGATCACGACATGCGGAAAGTCAATATTCGTC
AGAGCCAGACAGTGAGCTTCTCATGCTGCCCAGAGCAAAACGAGGACGAAGTGCAGAATG
CCTACACATGACCAGTGAACTGCAGCCCTCTCTTGACAGGGCTAGGAGTGCTAGTACCAA
CTGCTTGAGACCAGATACTAGTTTGCATTCACCAGAACGAGAAAGGGGTAGATGGTCCCC
CTCCCTAGCTAGGAGGCGACCTGCTAGCCCCAGGATTCAAATCCAGCATGCGTCTCCGGA
GAATGACAGGCACTCCAGAAAGTCTGAAAGATCTAGCATCCAAAAACAGTCTAGGAAAGG
CACAGCCTCTGATGCAGACAGGGTTCTCCCACCATGCCTTTCTAGAAGGGGATACGCAAT
CCCAAGAGCAACCGATCAACCGGTCATTAGGGGAAAGCATACCACTCGCTCCCGGTCGAG
CGAGCACTCTAGTATCAGAACCCTGTGTTCTATGCACCACCTTGCCCCCGGAGGGTCAGC
GCCGCCTTCTCCGCTTCTGACAAGAACGCACCGACAAGGAAGCCCCACCCAGTCACCTCC
AGCTGACACATCCTTTGGCAGTCGCCGCGGAAGACAGCTCCCACAGGTGCCGGTCCGAAG
CGGCAGTATAGAACAAGCGAGCTTAGTAGTGGAGGAACGAACGAGACAGATGAAAATGAA
GGTACACCGATTTAAGCAGACAACAGGGTCTGGGTCTAGTCAAGAACTTGACCACGAGCA
ATACTCCAAGTACAACATACATAAAGATCAGTACAGAAGCTGTGATAACGCGTCCGCCAA
GTCATCAGATAGTGATGTCAGTGATGTGTCCGCCATTTCCAGAGCCAGCAGCACCTCACG
CCTCAGCAGCACAAGCTTTATGTCAGAGCAGTCTGAGCGCCCCAGGGGCAGGATCAGTTC
ATTTACCCCCAAAATGCAAGGCAGACGGATGGGGACTTCAGGAAGAGCCATCATCAAGAG
CACCAGTGTAAGTGGAGAGATATATACACTGGAGCACAATGACGGCAGCCAGTCCGACAC
GGCCGTGGGTACCGTTGGAGCTGGTGGAAAGAAGCGGAGGTCCAGCCTGAGTGCCAAAGT
GGTAGCCATTGTGTCTCGAAGAAGCAGAAGCACATCACAGCTCAGCCAGACAGAGTCCGG
CCACAAGAAGTTAAAAAGCACCATTCAGAGGAGTACGGAAACGGGAATGGCGGCTGAAAT
GCGGAAGATGGTGAGACAGCCCAGCCGGGAGTCCACGGATGGCAGCATCAACAGTTATAG
CTCAGAAGGAAACTTAATATTTCCTGGAGTTCGCGTGGGACCTGACAGTCAGTTCAGTGA
TTTCCTTGATGGACTGGGGCCAGCCCAGCTTGTCGGCCGCCAAACCCTAGCCACCCCAGC
CATGGGCGATATCCAAATCGGGATGGAGGATAAGAAGGGTCAGTTGGAGGTTGAGGTTAT
CAGAGCCCGGAGCCTTACACAAAAACCTGGCTCCAAATCTACACCTGCTCCCTATGTGAA
AGTTTATCTTTTGGAAAATGGAGCCTGTATTGCCAAAAAGAAGACAAGAATTGCACGGAA
AACTCTCGATCCCTTGTATCAGCAGTCCCTGGTTTTTGATGAAAGTCCACAGGGTAAAGT
TCTTCAGGTGATTGTCTGGGGTGACTATGGAAGAATGGACCACAAATGCTTTATGGGTGT
GGCTCAAATCTTGTTGGAAGAACTTGATCTGTCCAGCATGGTGATTGGATGGTATAAATT
GTTCCCTCCATCCTCCCTGGTGGATCCCACACTCACTCCCCTGACCCGCCGGGCTTCCCA
ATCATCACTGGAAAGTTCGTCTGGGCCTCCCTGCATCCGGTCATAGGGCCTCATGGGCCC
AGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MSSAVGPRGPRPPTVPPPMQELPDLSHLTEEERNIIMAVMDRQKEEEEKEEAMLKCVVRD MAKPAACKTPRNAESQPHQPPLNIFRCVCVPRKPSSEEGGPDRNWRLHQQFESYKEQVRK IGEEARRYQGEHKDDAPTCGICHKTKFADGCGHLCSYCRTKFCARCGGRVSLRSNNEDKV VMWVCNLCRKQQEILTKSGAWFFGSGPQQPSQDGTLSDTATGAGSEVPREKKARLQERSR SQTPLSTAAVSSQDTASHGAPLDRNKGAEPSQQALGPEQKQASRSRSEPPRERKKAPGLS EQNGKGGQKSERKRVPKSVVQPGEGTADERERKERRETRRLEKGRSQDYPDRLEKREDGR VAEDEKQRKEEEGVSTPEYTSCEDVELESESVSEKGDLDYWLDPATWHSRETSPISSHPV TWQPSKEGDRLIGRVILNKRTTMPKESGALLGLKVVGGKMTDLGRLGAFITKVKKGSLAD VVGHLRAGDEVLEWNGKPLPGATNEEVYNIILESKSEPQVEIIVSRPIGDIPRIPESSHP PLESSSSSFESQKMERPSISVISPTSPGALKDAPQVLPGQLSVKLWYDKVGHQLIVNVLQ ATDLPPRVDGRPRNPYVKMYFLPDRSDKSKRRTKTVKKLLEPKWNQTFVYSHVHRRDFRE RMLEITVWDQPRVQDEESEFLGEILIELETALLDDEPHWYKLQTHDESSLPLPQPSPFMP RRHIHGESSSKKLQRSQRISDSDISDYEVDDGIGVVPPVGYRASARESKATTLTVPEQQR TTHHRSRSVSPHRGDDQGRPRSRLPNVPLQRSLDEIHPTRRSRSPTRHHDASRSLADHRS RHAESQYSSEPDSELLMLPRAKRGRSAECLHMTSELQPSLDRARSASTNCLRPDTSLHSP ERERGRWSPSLARRRPASPRIQIQHASPENDRHSRKSERSSIQKQSRKGTASDADRVLPP CLSRRGYAIPRATDQPVIRGKHTTRSRSSEHSSIRTLCSMHHLAPGGSAPPSPLLTRTHR QGSPTQSPPADTSFGSRRGRQLPQVPVRSGSIEQASLVVEERTRQMKMKVHRFKQTTGSG SSQELDHEQYSKYNIHKDQYRSCDNASAKSSDSDVSDVSAISRASSTSRLSSTSFMSEQS ERPRGRISSFTPKMQGRRMGTSGRAIIKSTSVSGEIYTLEHNDGSQSDTAVGTVGAGGKK RRSSLSAKVVAIVSRRSRSTSQLSQTESGHKKLKSTIQRSTETGMAAEMRKMVRQPSRES TDGSINSYSSEGNLIFPGVRVGPDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQIGMEDK KGQLEVEVIRARSLTQKPGSKSTPAPYVKVYLLENGACIAKKKTRIARKTLDPLYQQSLV FDESPQGKVLQVIVWGDYGRMDHKCFMGVAQILLEELDLSSMVIGWYKLFPPSSLVDPTL TPLTRRASQSSLESSSGPPCIRS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
4392
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
