Gene:
Gene Symbol:
Pcdha6
Gene Name:
protocadherin alpha 6
Original Clone ID:
FLH481795.01X
Keyword:
None
Species:
Mus musculus
Type:
cDNA
Vector Name:
pENTR223.1
Format:
CLOSED
Source:
The ORFeome Collaboration
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The ORFeome Collaboration
MmCD00297476
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 2841nts
Open reading frame: 35 to 2875
GTACAAAAAAGCAGAAGGGCCGTCAAGGCCCACCATGGATTTTACCACTGAAGGCCGACT
GGGAACACGGTGTATGCTGCTCTCGCTCCTGCTCCTCGCAGCCTGGGAGGCAGGGAGCGG
CCAGCTTCATTACTCCGTCCCAGAGGAGGCCAAACACGGCACCTTCGTGGGCCGCATCGC
GCAGGACCTGGGGCTGGAGCTGACGGAGCTGGTGCCCCGCCTGTTCAGGGTGGCGTCCAA
GGACCGCGGGGACCTTCTGGAGGTAAATCTGCAGAATGGCATTTTGTTTGTGAATTCTCG
GATCGACCGGGAGGAGCTGTGCGGGCGGAGCGTGGAGTGTAGCATCCACCTGGAGGTGAT
CGTGGACAGGCCGCTGCAGGTTTTCCACGTGGAGGTGGAGGTGAGGGACATTAATGATAA
CCCGCCTGTGTTCTCAGTAAAGGAGCAAAGAATGTTAATTTACGAATCTAGGCTGCCAGA
TTCTTTGTTTCCACTAGAGGGCGCATCAGATGCTGATGTTGGATTAAATTCTATGTTAAC
CTATAAACTCAGTCCCAGCGAATACTTTGGGCTAGATGTGAAATCAAACAGCGATGGCAA
CAAACAAATTAGACTCTTGTTGAAAAAATCCTTGGACAGAGAGGATGCTCCTGAGCATAA
GCTACTGCTGACAGCCACGGATGGAGGCAAACCTGAGCTCACAGGCTCTGTTCAGCTGCT
GGTCACTGTGCTGGATGTGAATGATAACGCCCCTACTTTCCAGCACCCTGAGTATGAAGT
GAGAATCTTGGAAAACTCAGACAACGGAACGACAGTTATTAGACTGAATGCTTCTGACAA
GGATGAAGGGACGAATTCAGCAATTTCGTACTCTTTTAATAGACTGGTGCCGCCCAAGAC
TCTTGAGCAGTTTAGCATAGATGCAGATACAGGAGAAATCATAACTCAAGGAAATTTGGA
CTTTGAGCAAGTAGATGTCTACAAAATCCACGTTGATGCGACGGACAAAGGACACCCTCC
GATGGTGGGCCATTGCACTGTTTTAGTGAAGGTATTGGATGAGAACGACAATGTGCCTCA
GATCACTTTGACTTCCTTATCCCTGCCTGTTCGAGAGGACGCTGCACTGAGTACTGTCAT
TGCTCTAATTAGCGTTTCGGATCTGGACTCTGGATCCAACGGGCAGGTGACCTGCTCCCT
GAGTCCTCATGTCCCCTTCAAGTTGGTGTCCACCTTCAAGAATTACTATTCGCTTGTGTT
GGACAGCGCCCTGGACCGAGAGACCACAGCTGACTATCAGGTGGTGGTGACAGCGCGGGA
CGGGGGTTCTCCTTCTTTGTGGACCACGGCTAGCGTGTCTGTGGAGGTGGCCGATGTGAA
CGACAATGCGCCTTCATTTGCGCAGCCTGATTACACGGTGTTCGTGAAGGAGAACAACCC
TCCTGGAGCGCACATCTTCACAGTATCGGCGATGGACGCGGATGCGCAGGAGAACGCGCT
GGTGTCCTACTCGCTGGTGGAGCGGAGGGTGGGCGAGCGCTTGCTGTCGAGCTATGTGTC
TGTGCACGCGGAAAGCGGCAAGGTGTTCGCTCTGCAGCCTCTGGACCATGAGGAGCTGGA
ACTGCTGCAGTTCCAGGTGAGCGCGCGGGATGCTGGTGTGCCTGCCCTGGGCAGCAATGT
GACTCTGCAGGTGTTTGTGCTGGATGAGAACGACAATGCGCCCACGCTGCTTGGACCTTG
GACACCAGGATCTGGTGGTGTAGTGAGCGAACTTGTGTCACCTTCAGTGGGTGCTGGCCA
GGTGGTCACAAAGGTGCGTGCAGTAGATGCGGATTCTGGCTATAATGCCTGGCTGTCTTA
TGAGCTGCATCCTTTAGCAGGTGGAACGCGGAGTCTGTTTAGGGTAGGTCTGTACACGGG
CGAGATCAGTACCACGCGTGCCCTTGAGGAGGCAGATGCACCACGCCATCGCCTATTAGT
GCTGGTGAAGGACCACGGGGAGCCAGCGCTGATTGCCACAGCCACTGTGTTAGTGTCTCT
GGTTGAGAACAGCCAGACTCCAAAAGCCTCTTCGCGAGCGCAAGGTGCCACTGGCCAGGA
AGTTTCGTTAGTGAATGTCAACGTGTACCTGATCATTGCCATCTGTGCGGTGTCCAGCCT
ACTAGTTCTAACCCTCATGGTATACACCGCACTGCGGTGCTCAGTTCCACCTACAGAGGG
TGTGTGCGGGGCAGGGAAGCCTGTGCTGGTGTGCTCTAGTGCGGTAGGGAGCTGGTCCTA
CTCACAGCAGAGGAGACAGAAGGTTTGTTCTGGAGAGGGACCACCCAAAACCGATCTCAT
GGCCTTCAGCCCGAGTCTTCCTCCTTGTCCGGTTGTGGGTGAGCATCAGGATTTGAACGA
CGATCATTGCTCAAGACCCCGGCAGCCCAACCCTGACTGGCGCTACTCTGCCTCGCTAAG
AGCAGGCATGCACAGCTCTGTGCACCTGGAGGAGGCTGGCATTCTACGGGCTGGTCCAGG
AGGGCCTGATCAGCAGTGGCCAACAGTATCCAGTGCAACACCAGAACCTGAGGCAGGAGA
GGTGTCCCCTCCGGTGGGCGCCGGTGTCAACAGCAACAGCTGGACCTTTAAATACGGACC
AGGCAACCCCAAACAGTCCGGTCCCGGTGAGTTGCCAGACAAATTCATTATCCCAGGATC
TCCTGCAATCATCTCCATCCGGCAGGAGCCTGCTAACAACCAAATTGACAAAAGCGATTT
TATAACCTTCGGCAAAAAGGAGGAGACCAAGAAAAAGAAGAAAAAGAAGAAGGGTAACAA
GACCCAGGAGAAAAAAGAGAAAGGGAACAGCACGACGGACAACAGTGACCAGTAGGGCCT
CATGGGCCCAGCTTTCTTGTAC
Protein sequence predicted based on nucleotide sequence
MDFTTEGRLGTRCMLLSLLLLAAWEAGSGQLHYSVPEEAKHGTFVGRIAQDLGLELTELV PRLFRVASKDRGDLLEVNLQNGILFVNSRIDREELCGRSVECSIHLEVIVDRPLQVFHVE VEVRDINDNPPVFSVKEQRMLIYESRLPDSLFPLEGASDADVGLNSMLTYKLSPSEYFGL DVKSNSDGNKQIRLLLKKSLDREDAPEHKLLLTATDGGKPELTGSVQLLVTVLDVNDNAP TFQHPEYEVRILENSDNGTTVIRLNASDKDEGTNSAISYSFNRLVPPKTLEQFSIDADTG EIITQGNLDFEQVDVYKIHVDATDKGHPPMVGHCTVLVKVLDENDNVPQITLTSLSLPVR EDAALSTVIALISVSDLDSGSNGQVTCSLSPHVPFKLVSTFKNYYSLVLDSALDRETTAD YQVVVTARDGGSPSLWTTASVSVEVADVNDNAPSFAQPDYTVFVKENNPPGAHIFTVSAM DADAQENALVSYSLVERRVGERLLSSYVSVHAESGKVFALQPLDHEELELLQFQVSARDA GVPALGSNVTLQVFVLDENDNAPTLLGPWTPGSGGVVSELVSPSVGAGQVVTKVRAVDAD SGYNAWLSYELHPLAGGTRSLFRVGLYTGEISTTRALEEADAPRHRLLVLVKDHGEPALI ATATVLVSLVENSQTPKASSRAQGATGQEVSLVNVNVYLIIAICAVSSLLVLTLMVYTAL RCSVPPTEGVCGAGKPVLVCSSAVGSWSYSQQRRQKVCSGEGPPKTDLMAFSPSLPPCPV VGEHQDLNDDHCSRPRQPNPDWRYSASLRAGMHSSVHLEEAGILRAGPGGPDQQWPTVSS ATPEPEAGEVSPPVGAGVNSNSWTFKYGPGNPKQSGPGELPDKFIIPGSPAIISIRQEPA NNQIDKSDFITFGKKEETKKKKKKKKGNKTQEKKEKGNSTTDNSDQ*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
2841
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pENTR223.1
Synonyms:
pDONR-223.1, pENTR223-star-Sfi
Description:
Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Comments:
Additional information about this vector is available from the commercial website and at the ORFeome website http://orfeomecollaboration.org/vectors.php
Size (bp):
4311
Parent Vector:
None
Properties:
Gateway, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
MCS (Sfi sites flank the cassette present only in no insert form)
1265
1277
bacterial origin
ori
bacterial origin of replication
1
534
negative selection marker
ccdB
ccdB (no insert form only; lost in with insert form)
1312
1617
primer site
M13 F
M13 forward primer for insert sequencing
1113
1129
primer site
T7
T7 Forward primer for insert sequencing
2902
2921
primer site
M13 reverse
M13 reverse primer for insert sequencing
2926
2942
recombination site
attL1
attL1 recombination site
1165
1264
recombination site
attL2
attL2 recombination site
2786
2885
selectable marker
CmR
chlR (no insert form only; lost in with insert form)
1959
2618
selectable marker
SpnR
spectinomycin resistance gene
3154
4164
