Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
2482.1.18_GO.81370
Keyword:
None
Species:
Cyanidioschyzon merolae strain 10D
Type:
cDNA
Vector Name:
pVP65K
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
MBP-His8 fusion protein; MPB is cleaved in vivo by TVMV protease. TEV cleavage site that is downstream from the SgfI site is used to remove His8 tag. after TEV cleavage the protein obtained will be the translated CDS with the addition of S
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
CmCD00297615
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 414nts
Open reading frame: 50 to 463

CTTCCAGTCCCTTCCTATCGCGTCTACTGCGACGCAGATGGGGAGCAACAAGACCAGAAT
ATTTGCTCGACAGCCGGCAAAAGCCCCACCGCCTCTGGCCTGGTTGAGTCGACCCCGAGA
CCCTCGAGCAACAGCGATTGCAGCTGCGGGCCAACAGTCCTCGCGACGGGTCAGTAAACG
AGAACTGGAGTCACATGGCAGTGCTACTGACGCCTGGACGGCTATCCACGGAGTTGTGTA
TGACATAACCGCTTACTTCAGATTCCACCCTGGTGGTGCGGAAATCCTGTTCATAGAAGC
GGCCGGAAAGGATAGCACAGCTGTGTTTGATGAGATGCACCCGTGGGTGAACGTGGCGTG
GCTCCTAAAAGACTATCGAATAGGACTCTATGCGCCCGAGTCGGAGGACGACGGCGGAGA
CGACGACGACGACGACCGGACGATGACTAGTCCAGAATGATAGTTTAAACGAATTCGAGC
TCGGTACCCGGGGATCCTCTAGAGTCGACCTG

Protein sequence predicted based on nucleotide sequence

KTRIFARQPAKAPPPLAWLSRPRDPRATAIAAAGQQSSRRVSKRELESHGSATDAWTAIH
GVVYDITAYFRFHPGGAEILFIEAAGKDSTAVFDEMHPWVNVAWLLKDYRIGLYAPESED
DGGDDDDDDRTMTSPE**

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 483
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.81370
Recommended expression in:
Not Applicable