Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
10109f6BSt7p1
Keyword:
None
Species:
Vibrio cholerae
Type:
cDNA
Vector Name:
pSGX4
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
N-terminal 6-His and Smt3, cleavable, TOPO
Mutation / Discrepancy:
Yes / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
VcCD00297935
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AVAILABLE
No restriction
Special MTA: PSI/Smt3/SUMO
Insert sequence: 711nts
Open reading frame: 50 to 760

GGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTGAACAAAAAAT
CCTAGCTCAAGGCGCATTGGAAGCGGCTCGCCGCCGCCAGTCGGAGCAAGAAAAAGCGCT
GTTATTGAGTCAACTCAAGCAGCTACAAAGTCAGATGGAGCCTCATTTTCTATTCAATAC
TTTGGCCAATATTAATGCTTTGATTGCGGTAGAACCCTATAAAGCGCAGTTAATGTTGGA
GAAATTCACTGAGTTACTGCGCGGTACTATGCGCTTGCGACGAACCAATACAGGAGACTT
GCGTGAAGAGATGCAACTGATTGATGCGTATCTGGGAATTCAAAAAATACGCTTAGGGGA
AAGGCTGGAATACACTCTGCCCGAGTTGTCAGAATTGGGTACACTCGGCATGCCGCCTAT
GTTGATTCAACCTTTGGTCGAAAATGCAGTGTCACATGGTATTGAACCTAAAGCCGAAGG
CGGTACTATTCGAGTGAGAATCGAAGTGGCTGATGACTGGTTTGAGCTATCGGTAGAAGA
TAATGGCATGGGCTTAAGTGATACCCCCAAGGGCAATGGTATTGCTTTGCAGAATGTTCG
CGATCGACTGTCTGGGCTCTTTGGACATGAAGGTTTACTTACCGTGGCGCAAAATTGCTC
TGGCGGCGTTACGGCCACGCTTCGCATCCGTTTGCACCATTTGCAGGGATTGCAGCGTTC
TTTTTATGATTACTCGCAGGCTCGTTCACCTCAATCGTAAGGGTA

Protein sequence predicted based on nucleotide sequence

EQKILAQGALEAARRRQSEQEKALLLSQLKQLQSQMEPHFLFNTLANINALIAVEPYKAQ
LMLEKFTELLRGTMRLRRTNTGDLREEMQLIDAYLGIQKIRLGERLEYTLPELSELGTLG
MPPMLIQPLVENAVSHGIEPKAEGGTIRVRIEVADDWFELSVEDNGMGLSDTPKGNGIAL
QNVRDRLSGLFGHEGLLTVAQNCSGGVTATLRIRLHHLQGLQRSFYDYSQARSPQS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 711
Mutations:
L189F;

Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-10109f
Recommended expression in:
Not Applicable