Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
10382d2BCt12p1
Keyword:
None
Species:
Thermobifida fusca YX
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
Yes / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
TfCD00297987
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1332nts
Open reading frame: 50 to 1381
CCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAGAAGAGATCA
TACCCGTACGGCGCAGGTGACTCACGTTGAAAGCCTGACAAATTCTCCGGGCGTACGCGA
GAACTGGCCAGGTTGGGCCAGTCCTGCAGTTGTAGAACGTATGGTCAAATCAGGGGTGGC
TAAACCGTGGTCCCATCAAGTCCGCGCGGCAAATCTGGCCCATTCTGGATCGAACGTGAT
TATCGCTACCGGCATTAGCAGTGGTAAATCACTGGGGTTTCTGATGCCATGCACGGCGGC
CATCGCAGAGGGAGGCTCCGTTCTGTATCTGGCTCCTACTAAAGCGCTGGCCGCAGACCA
GGAACGTAAACTGGCTGAACTGGCGATGCCGGGTGTCCGCGCCGCAGTATACGATGGCGA
CACACCGATGGAAGAGCGTTCGTGGGCTCGTGCACATGCTAATTATGTGCTGACCAACCC
AGATATGCTGCACCGCAGCATTGTTGCGCGCCACCCTGTGTGGGCCGGTTTCCTGCGTCG
TCTGTCTTACGTCGTTGTAGACGAGGCGCACCATTATCGCGGGGTTTTTGGAAGTCACGT
GGCCCAAATCATGCGCCGTCTGCGCCGTGTATGTGGCTGCTACGGTGCAGAACCGCGTTT
CATTCTGGCGTCAGCTACGACCGCCACTCCAGCAGAAACAGCGCAACAGCTGACTGGCCT
GCCTGTTGTAGCTGTTACGGGTGATGGGTCTGCCCGTGAAAGTCTGCGTTTTGCGCTGGT
AGAACCGGAACTGACAGAGAAAACCGGAGAGCAGGGCGCTCCAATGCGTCGTACTGCAAC
AGCCGTTGCTGCAGAACTGCTGGCTGATCTGGTATCCCATGAGGTGAGCACCCTGGTTTT
CGTCCGCTCACGCCAAGCAGCTGAACTGGTTGCGCTGGCAGCTCAACGTGCACTGGCTGA
TGCAGGTCGTCCTGAACTGGCTCGTCGTGTAGCAGCTTATCGTGGAGGTTACCTGGCAGA
AGATCGCCGTCGTCTGGAACAACGTCTGCGTTCTGGTGAACTGCTGGGACTGGCAACTAC
AAATGCGCTGGAGCTGGGCGTTGATGTGTCGGGTCTGGATGCCGTAGTTATGGCGGGTTG
GCCAGGAACTCGTGCTAGTCTGTGGCAACAGGCAGGTCGTGCTGGTCGTTCAGGACGTGA
TGCACTGGCTGTATTTGTGGCCCGTGATGATCCGCTGGATACATATCTGGTCCACCATCC
GGACGCGATCTTCGGCCAAGACGTGGAAGCCACTGTCCTGGACCCGGATAACCCGTATGT
GCTGGCGCCTCATCTGTGTGCCGCAGCTGAAGAAGAAGGGCATCATCACCATCACCACTG
ATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCAC
Protein sequence predicted based on nucleotide sequence
RRDHTRTAQVTHVESLTNSPGVRENWPGWASPAVVERMVKSGVAKPWSHQVRAANLAHSG SNVIIATGISSGKSLGFLMPCTAAIAEGGSVLYLAPTKALAADQERKLAELAMPGVRAAV YDGDTPMEERSWARAHANYVLTNPDMLHRSIVARHPVWAGFLRRLSYVVVDEAHHYRGVF GSHVAQIMRRLRRVCGCYGAEPRFILASATTATPAETAQQLTGLPVVAVTGDGSARESLR FALVEPELTEKTGEQGAPMRRTATAVAAELLADLVSHEVSTLVFVRSRQAAELVALAAQR ALADAGRPELARRVAAYRGGYLAEDRRRLEQRLRSGELLGLATTNALELGVDVSGLDAVV MAGWPGTRASLWQQAGRAGRSGRDALAVFVARDDPLDTYLVHHPDAIFGQDVEATVLDPD NPYVLAPHLCAAAEEEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
1332
Mutations:
L90M; L124M; L199M; L270M;
L372M;
Discrepancy:
"c710a,A237E;"
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-10382d
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
