Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
9387a1BCt5p1
Keyword:
None
Species:
Sinorhizobium meliloti
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
C-terminal 6-His, TOPO
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SmCD00298232
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1146nts
Open reading frame: 50 to 1195

CCCTCTGAATATTTTGTTTACTTAAGAAGGAGATATACATATGTCCCTTGTGAAAATTAG
CAATGTGCGTGTGCGCCCGCTGGTATTACCATTGAAGCAGCCTTATCATTGGTCTTACGG
CATCCGGGAAAGTTTTGCGGTGAACCTTATAGAGATTGAAGCCGATGACGGTACCGTTGG
GATCGGAGAGTGCACGGTCGCACCCGATCAAACTGGCACAGCTGCGATTCTCTATAGACT
GGCCAAACACCTGGTAGGTCATTCACCGCACGACGTGGCACCATTAATCGCTCGAATATT
CCATCAGGAATACTTGGGGCACGGAGCGAATATTATGAGAGCAGCCAACCAAATCTTTTC
CGGCATTGATATGGCTATGTGGGACCTTCAGGGTAAACTGGCAGGGCTTCCTGTTCATCA
GCTGTTAGGTGGGGCCCACCGCAAGGCAGTCGGATATTTCTACTTTCTGCAAGGCGAAAC
CGCTGAGGAATTGGCGCGTGATGCCGCAGTGGGTCATGCGCAGGGCGAGCGCGTATTCTA
TCTTAAAGTTGGTCGGGGGGAAAAACTGGATCTCGAAATAACGGCTGCCGTGCGTGGAGA
GATCGGCGACGCACGCTTAAGACTGGATGCGAATGAAGGTTGGTCGGTCCACGACGCTAT
TAACATGTGTCGAAAGTTGGAGAAATACGATATCGAATTTATTGAACAACCGACTGTTAG
CTGGTCTATACCCGCCATGGCACATGTACGTGAGAAAGTGGGAATCCCGATTGTCGCGGA
CCAGGCTGCCTTCACACTTTATGATGTTTATGAAATATGCCGCCAGCGTGCTGCAGATAT
GATCTGTATTGGCCCACGTGAGATTGGTGGAATCCAACCTATGATGAAGGCCGCAGCGGT
GGCTGAAGCCGCCGGATTAAAAATTTGCATACACAGTTCATTTACCACGGGTATCACCAC
TTGTGCTGAACATCATATTGGGTTAGCAATCCCGAATCTGGACGATGGCAACCAGATTAT
GTGGCAACTCGTACAGGAGGACATAGTCTCCTCGCCCGATCTGACACCGAAAAATGGTTG
GTTGGATGCCTTCCGCAAGCCAGGACTTGGCTTTCAGCTGGCGGAGGACTTAGTTGCTGA
AGGTGAAGGGAGATATGCAGCCTCTAGAGAAGGGCATCATCACCATCACCACTGATCCGA
ATTCGAGCTCCGTCGACAAGCTTGCGACCGCACTCGAGCACCAC

Protein sequence predicted based on nucleotide sequence

VKISNVRVRPLVLPLKQPYHWSYGIRESFAVNLIEIEADDGTVGIGECTVAPDQTGTAAI
LYRLAKHLVGHSPHDVAPLIARIFHQEYLGHGANIMRAANQIFSGIDMAMWDLQGKLAGL
PVHQLLGGAHRKAVGYFYFLQGETAEELARDAAVGHAQGERVFYLKVGRGEKLDLEITAA
VRGEIGDARLRLDANEGWSVHDAINMCRKLEKYDIEFIEQPTVSWSIPAMAHVREKVGIP
IVADQAAFTLYDVYEICRQRAADMICIGPREIGGIQPMMKAAAVAEAAGLKICIHSSFTT
GITTCAEHHIGLAIPNLDDGNQIMWQLVQEDIVSSPDLTPKNGWLDAFRKPGLGFQLAED
LVAEGEGRYAASREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence 1
End on reference sequence 1146
Mutations:
None
Discrepancy:
"c289a,t291a,R97R;c294a,A98A;
a297c,A99A;g354a,A118A;
c357g,G119G;c360t,L120L;
t714a,G238G;g780t,R260R;
a783t,A261A;g786a,A262A;
g864c,A288A;c867a,G289G;
c868t,g870a,L290L;a1077g,E359E;
"
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9387a
Recommended expression in:
Not Applicable