STK23 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SRPK3
Gene Name:  SRSF protein kinase 3
Original Clone ID:
FLH230700.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00298577
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1701nts
Open reading frame: 1 to 1701

ATGAGCGCCAGCACGGGCGGTGGTGGGGACAGCGGCGGCAGCGGCGGCAGTAGCAGCAGC
TCACAGGCCTCCTGCGGGCCCGAGTCCTCGGGCTCCGAACTAGCCCTGGCCACACCGGTG
CCTCAGATGCTGCAGGGCCTTCTGGGCTCCGACGACGAGGAACAGGAAGACCCCAAAGAC
TACTGCAAGGGCGGCTACCACCCTGTGAAGATCGGCGACGTGTTCAATGGGCGGTACCAC
GTGGTGCGCAAACTGGGCTGGGGCCACTTCTCCACCGTCTGGCTCTGCTGGGACATCCAG
CGCAAGCGCTTTGTGGCCCTCAAAGTGGTGAAGAGTGCGGGGCATTACACGGAGACAGCT
GTGGATGAGATCAAGCTCCTGAAATGTGTCCGGGACAGCGACCCCAGTGACCCCAAAAGA
GAGACCATTGTCCAGCTCATTGATGACTTCAGGATCTCAGGAGTCAATGGAGTCCATGTG
TGCATGGTGCTGGAGGTGCTGGGCCACCAGCTCCTCAAATGGATCATCAAGTCCAACTAC
CAGGGCCTGCCCGTGCCCTGCGTGAAGAGCATCGTGAGGCAGGTGCTGCACGGCCTGGAC
TACCTCCACACCAAGTGCAAGATCATCCACACGGACATCAAGCCCGAGAACATCTTGCTG
TGTGTGGGGGACGCTTACATCAGGCGCCTGGCTGCCGAGGCCACGGAGTGGCAACAGGCA
GGGGCGCCGCCCCCCTCCCGCTCCATAGTCAGCACTGCCCCCCAGGAGGTCTTGACCGGT
AAGCTGTCCAAAAACAAGAGGAAGAAGATGAGGCGCAAACGGAAACAGCAGAAGCGGCTG
CTGGAGGAGCGGCTGCGGGACCTGCAGAGGCTGGAGGCCATGGAGGCTGCCACCCAGGCT
GAGGACTCTGGCTTGAGACTAGACGGGGGCAGCGGCTCCACATCCTCTTCAGGCTGTCAC
CCCGGGGGCGCCAGAGCAGGTCCCTCCCCAGCCTCTTCCTCCCCCGCCCCGGGGGGCGGC
CGTAGCCTCAGCGCGGGCTCACAGACCTCAGGCTTCTCCGGCTCCCTCTTCTCTCCTGCC
TCCTGCTCCATCCTCTCCGGCTCGTCCAATCAGCGAGAGACCGGGGGCCTCCTGTCGCCT
AGCACACCATTCGGTGCCTCGAACCTCCTGGTGAACCCCCTGGAGCCCCAAAATGCAGAT
AAGATCAAGATCAAGATCGCAGACCTGGGCAACGCCTGCTGGGTGCACAAGCACTTCACG
GAAGACATCCAGACTCGGCAGTACCGGGCCGTCGAGGTGCTGATCGGCGCCGAATACGGC
CCCCCGGCAGACATCTGGAGCACAGCCTGCATGGCCTTCGAGCTGGCCACTGGTGACTAC
CTGTTCGAGCCGCATTCTGGAGAAGACTACAGTCGTGATGAGGACCACATCGCTCACATA
GTGGAGCTTCTGGGGGACATCCCCCCAGCCTTCGCCCTCTCAGGCCGCTATTCCCGGGAG
TTCTTCAACCGGAGAGGAGAGCTGCGGCACATCCACAATCTCAAGCACTGGGGCCTGTAC
GAGGTACTCATGGAAAAGTACGAGTGGCCCCTAGAGCAGGCCACACAGTTCAGCGCCTTT
CTGCTGCCCATGATGGAGTACATCCCCGAAAAGCGGGCCAGTGCCGCTGACTGCCTCCAG
CACCCCTGGCTCAACCCCTTG

Protein sequence predicted based on nucleotide sequence

MSASTGGGGDSGGSGGSSSSSQASCGPESSGSELALATPVPQMLQGLLGSDDEEQEDPKD
YCKGGYHPVKIGDVFNGRYHVVRKLGWGHFSTVWLCWDIQRKRFVALKVVKSAGHYTETA
VDEIKLLKCVRDSDPSDPKRETIVQLIDDFRISGVNGVHVCMVLEVLGHQLLKWIIKSNY
QGLPVPCVKSIVRQVLHGLDYLHTKCKIIHTDIKPENILLCVGDAYIRRLAAEATEWQQA
GAPPPSRSIVSTAPQEVLTGKLSKNKRKKMRRKRKQQKRLLEERLRDLQRLEAMEAATQA
EDSGLRLDGGSGSTSSSGCHPGGARAGPSPASSSPAPGGGRSLSAGSQTSGFSGSLFSPA
SCSILSGSSNQRETGGLLSPSTPFGASNLLVNPLEPQNADKIKIKIADLGNACWVHKHFT
EDIQTRQYRAVEVLIGAEYGPPADIWSTACMAFELATGDYLFEPHSGEDYSRDEDHIAHI
VELLGDIPPAFALSGRYSREFFNRRGELRHIHNLKHWGLYEVLMEKYEWPLEQATQFSAF
LLPMMEYIPEKRASAADCLQHPWLNPL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1626
Start on reference sequence 25
Mutations:
None
Discrepancy:
ins@948,99;
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable