LRRC5 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  LRRC8D
Gene Name:  leucine rich repeat containing 8 family, member D
Original Clone ID:
FLH232456.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
HsCD00298951
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2577nts
Open reading frame: 1 to 2577

ATGTTTACCCTTGCGGAAGTTGCATCACTTAATGACATTCAGCCAACTTACCGAATCCTG
AAACCATGGTGGGATGTGTTTATGGATTACCTAGCTGTTGTTATGTTAATGGTAGCCATC
TTTGCAGGAACCATGCAACTTACCAAAGATCAGGTGGTCTGTTTGCCAGTATTGCCATCT
CCTGTAAATTCAAAGGCACATACACCACCAGGAAATGCCGAGGTCACCACCAACATCCCA
AAGATGGAAGCAGCCACCAACCAAGACCAAGATGGGCGGACAACAAACGACATTTCCTTT
GGGACATCTGCTGTGACACCTGACATACCTCTCAGAGCCACATATCCTCGCACAGATTTC
GCACTTCCAAATCAGGAGGCAAAGAAAGAGAAGAAAGATCCAACAGGTCGAAAAACAAAC
TTGGATTTTCAGCAATATGTATTTATTAATCAAATGTGTTACCATCTGGCCCTTCCGTGG
TATTCTAAGTACTTTCCATACCTAGCTCTTATACATACTATTATTCTCATGGTCAGTAGC
AACTTTTGGTTCAAATATCCCAAAACATGCTCAAAAGTAGAACATTTTGTTTCAATATTA
GGAAAGTGCTTTGAATCCCCTTGGACGACAAAAGCGTTGTCTGAGACAGCATGCGAAGAC
TCAAAGGAAAACAAGCAGAGAATAACAGGTGCCCAGACTCTACCAAAGCATGTTTCTACC
AGCAGTGATGAAGGGAGCCCCAGTGCCAGTACACCAATGATCAATAAAACTGGCTTTAAA
TTTTCAGCTGAGAAGCCTGTGATTGAAGTTCCCAGCATGACAATCCTGGATAAAAAGGAT
GGAGAGCAGGCCAAAGCCCTGTTTGAGAAAGTGAGGAAGTTCCGTGCCCATGTGGAAGAT
AGTGACTTGATCTATAAACTCTATGTGGTCCAAACAGTTATCAAAACAGCCAAGTTCATT
TTTATTCTCTGCTATACAGCGAACTTTGTCAACGCAATCAGCTTTGAACACGTCTGCAAG
CCCAAAGTTGAGCATCTGATTGGTTATGAGGTATTTGAGTGCACCCACAATATGGCTTAC
ATGTTGAAAAAGCTTCTCATCAGTTACATATCCATTATTTGTGTTTATGGCTTTATCTGC
CTCTACACTCTCTTCTGGTTATTCAGGATACCTTTGAAGGAATATTCTTTCGAAAAAGTC
AGAGAAGAGAGCAGTTTTAGTGACATTCCAGATGTCAAAAACGATTTTGCGTTCCTTCTT
CACATGGTAGACCAGTATGACCAGCTATATTCCAAGCGTTTTGGTGTGTTCTTGTCAGAA
GTTAGTGAAAATAAACTTAGGGAAATTAGTTTGAACCATGAGTGGACATTTGAAAAACTC
AGGCAGCACATTTCACGCAACGCCCAGGACAAGCAGGAGTTGCATCTGTTCATGCTGTCG
GGGGTGCCCGATGCTGTCTTTGACCTCACAGACCTGGATGTGCTAAAGCTTGAACTAATT
CCAGAAGCTAAAATTCCTGCTAAGATTTCTCAAATGACTAACCTCCAAGAGCTCCACCTC
TGCCACTGCCCTGCAAAAGTTGAACAGACTGCTTTTAGCTTTCTTCGCGATCACTTGAGA
TGCCTTCACGTGAAGTTCACTGATGTGGCTGAAATTCCTGCCTGGGTGTATTTGCTCAAA
AACCTTCGAGAGTTGTACTTAATAGGCAATTTGAACTCTGAAAACAATAAGATGATAGGA
CTTGAATCTCTCCGAGAGTTGCGGCACCTTAAGATTCTCCACGTGAAGAGCAATTTGACC
AAAGTTCCCTCCAACATTACAGATGTGGCTCCACATCTTACAAAGTTAGTCATTCATAAT
GACGGCACTAAACTCTTGGTACTGAACAGCCTTAAGAAAATGATGAATGTCGCTGAGCTG
GAACTCCAGAACTGTGAGCTAGAGAGAATCCCACATGCTATTTTCAGCCTCTCTAATTTA
CAGGAACTGGATTTAAAGTCCAATAACATTCGCACAATTGAGGAAATCATCAGTTTCCAG
CATTTAAAACGACTGACTTGTTTAAAATTATGGCATAACAAAATTGTTACTATTCCTCCC
TCTATTACCCATGTCAAAAACTTGGAGTCACTTTATTTCTCTAACAACAAGCTCGAATCC
TTACCAGTGGCAGTATTTAGTTTACAGAAACTCAGATGCTTAGATGTGAGCTACAACAAC
ATTTCAATGATTCCAATAGAAATAGGATTGCTTCAGAACCTGCAGCATTTGCATATCACT
GGGAACAAAGTGGACATTCTGCCAAAACAATTGTTTAAATGCATAAAGTTGAGGACTTTG
AATCTGGGACAGAACTGCATCACCTCACTCCCAGAGAAAGTTGGTCAGCTCTCCCAGCTC
ACTCAGCTGGAGCTGAAGGGGAACTGCTTGGACCGCCTGCCAGCCCAGCTGGGCCAGTGT
CGGATGCTCAAGAAAAGCGGGCTTGTTGTGGAAGATCACCTTTTTGATACCCTGCCACTC
GAAGTCAAAGAGGCATTGAATCAAGACATAAATATTCCCTTTGCAAATGGGATTTTG

Protein sequence predicted based on nucleotide sequence

MFTLAEVASLNDIQPTYRILKPWWDVFMDYLAVVMLMVAIFAGTMQLTKDQVVCLPVLPS
PVNSKAHTPPGNAEVTTNIPKMEAATNQDQDGRTTNDISFGTSAVTPDIPLRATYPRTDF
ALPNQEAKKEKKDPTGRKTNLDFQQYVFINQMCYHLALPWYSKYFPYLALIHTIILMVSS
NFWFKYPKTCSKVEHFVSILGKCFESPWTTKALSETACEDSKENKQRITGAQTLPKHVST
SSDEGSPSASTPMINKTGFKFSAEKPVIEVPSMTILDKKDGEQAKALFEKVRKFRAHVED
SDLIYKLYVVQTVIKTAKFIFILCYTANFVNAISFEHVCKPKVEHLIGYEVFECTHNMAY
MLKKLLISYISIICVYGFICLYTLFWLFRIPLKEYSFEKVREESSFSDIPDVKNDFAFLL
HMVDQYDQLYSKRFGVFLSEVSENKLREISLNHEWTFEKLRQHISRNAQDKQELHLFMLS
GVPDAVFDLTDLDVLKLELIPEAKIPAKISQMTNLQELHLCHCPAKVEQTAFSFLRDHLR
CLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLT
KVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNL
QELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLES
LPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTL
NLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPL
EVKEALNQDINIPFANGIL

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 2956
Start on reference sequence 380
Mutations:
None
Discrepancy:
g664a,E222K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable