Gene:
Gene Symbol:
CHD4
Gene Name:
chromodomain helicase DNA binding protein 4
Original Clone ID:
FLH264706.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00299846
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCGTCGGGCCTGGGCTCCCCGTCCCCCTGCTCGGCGGGCAGTGAGGAGGAGGATATG
GATGCACTTTTGAACAACAGCCTGCCCCCACCCCACCCAGAGGACCCAGAAGAGGATTTG
TCAGAAACAGAGACTCCAAAGCTCAAGAAGAAGAAAAAGCCTAAGAAACCTCGGGACCCT
AAAATCCCTAAGAGCAAGCGCCAAAAAAAGGAGCGTATGCTCTTATGCCGGCAGCTGGGG
GACAGCTCTGGGGAGGGGCCAGAGTTTGTGGAGGAGGAGGAAGAGGTGGCTCTGCGCTCA
GACAGTGAGGGCAGCGACTATACTCCTGGCAAGAAGAAGAAGAAGAAGCTTGGACCTAAG
AAAGAGAAGAAGAGCAAATCCAAGCGGAAGGAGGAGGAGGAGGAGGAGGATGATGATGAT
GATTCAAAGGAGCCTAAATCATCTGCTCAGCTCCTGGAAGACTGGGGCATGGAAGACATT
GACCACGTGTTCTCAGAGGAGGATTATCGAACCCTCACCAACTACAAGGCCTTCAGCCAG
TTTGTCAGACCCCTCATTGCTGCCAAAAATCCCAAGATTGCTGTCTCCAAGATGATGATG
GTTTTGGGTGCAAAATGGCGGGAGTTCAGTACCAATAACCCCTTCAAAGGCAGTTCTGGG
GCATCAGTGGCAGCTGCGGCAGCAGCAGCGGTAGCTGTGGTGGAGAGCATGGTGACAGCC
ACTGAGGTTGCACCACCACCTCCCCCTGTGGAGGTGCCTATCCGCAAGGCCAAGACCAAG
GAGGGCAAAGGTCCCAATGCTCGGAGGAAGCCCAAGGGCAGCCCTCGTGTACCTGATGCC
AAGAAGCCTAAACCCAAGAAAGTAGCTCCCCTGAAAATCAAGCTGGGAGGTTTTGGTTCC
AAGCGTAAGAGATCCTCGAGTGAGGATGATGACTTAGATGTGGAATCTGACTTCGATGAT
GCCAGTATCAATAGCTATTCTGTTTCTGATGGTTCCACCAGCCGTAGTAGCCGCAGCCGC
AAGAAACTCCGAACCACTAAAAAGAAAAAGAAAGGCGAGGAGGAGGTGACTGCTGTGGAT
GGTTATGAGACAGACCACCAGGACTATTGCGAGGTGTGCCAGCAAGGCGGTGAGATCATC
CTGTGTGATACCTGTCCCCGTGCTTACCACATGGTCTGCCTGGATCCCGACATGGAGAAG
GCTCCCGAGGGCAAGTGGAGCTGCCCACACTGCGAGAAGGAAGGCATCCAGTGGGAAGCT
AAAGAGGACAATTCGGAGGGTGAGGAGATCCTGGAAGAGGTTGGGGGAGACCTCGAAGAG
GAGGATGACCACCATATGGAATTCTGTCGGGTCTGCAAGGATGGTGGGGAACTGCTCTGC
TGTGATACCTGTCCTTCTTCCTACCACATCCACTGCCTGAATCCCCCACTTCCAGAGATC
CCCAACGGTGAATGGCTCTGTCCCCGTTGTACGTGTCCAGCTCTGAAGGGCAAAGTGCAG
AAGATCCTAATCTGGAAGTGGGGTCAGCCACCATCTCCCACACCAGTGCCTCGGCCTCCA
GATGCTGATCCCAACACGCCCTCCCCAAAGCCCTTGGAGGGGCGGCCAGAGCGGCAGTTC
TTTGTGAAATGGCAAGGCATGTCTTACTGGCACTGCTCCTGGGTTTCTGAACTGCAGCTG
GAGCTGCACTGTCAGGTGATGTTCCGAAACTATCAGCGGAAGAATGATATGGATGAGCCA
CCTTCTGGGGACTTTGGTGGTGATGAAGAGAAAAGCCGAAAGCGAAAGAACAAGGACCCT
AAATTTGCAGAGATGGAGGAACGCTTCTATCGCTATGGGATAAAACCCGAGTGGATGATG
ATCCACCGAATCCTCAACCACAGTGTGGACAAGAAGGGCCACGTCCACTACTTGATCAAG
TGGCGGGACTTACCTTACGATCAGGCTTCTTGGGAGAGTGAGGATGTGGAGATCCAGGAT
TACGACCTGTTCAAGCAGAGCTATTGGAATCACAGGGAGTTAATGAGGGGTGAGGAAGGC
CGACCAGGCAAGAAGCTCAAGAAGGTGAAGCTTCGGAAGTTGGAGAGGCCTCCAGAAACG
CCAACAGTTGATCCAACAGTGAAGTATGAGCGACAGCCAGAGTACCTGGATGCTACAGGT
GGAACCCTGCACCCCTATCAAATGGAGGGCCTGAATTGGTTGCGCTTCTCCTGGGCTCAG
GGCACTGACACCATCTTGGCTGATGAGATGGGCCTTGGGAAAACTGTACAGACAGCAGTC
TTCCTGTATTCCCTTTACAAGGAGGGTCATTCCAAAGGCCCCTTCCTAGTGAGCGCCCCT
CTTTCTACCATCATCAACTGGGAGCGGGAGTTTGAAATGTGGGCTCCAGACATGTATGTC
GTAACCTATGTGGGTGACAAGGACAGCCGTGCCATCATCCGAGAGAATGAGTTCTCCTTT
GAAGACAATGCCATTCGTGGTGGCAAGAAGGCCTCCCGCATGAAGAAAGAGGCATCTGTG
AAATTCCATGTGCTGCTGACATCCTATGAATTGATCACCATTGACATGGCTATTTTGGGC
TCTATTGATTGGGCCTGCCTCATCGTGGATGAAGCCCATCGGCTGAAGAACAATCAGTCT
AAGTTCTTCCGGGTATTGAATGGTTACTCACTCCAGCACAAGCTGTTGCTGACTGGGACA
CCATTACAAAACAATCTGGAAGAGTTGTTTCATCTGCTCAACTTTCTCACCCCCGAGAGG
TTCCACAATTTGGAAGGTTTTTTGGAGGAGTTTGCTGACATTGCCAAGGAGGACCAGATA
AAAAAACTGCATGACATGCTGGGGCCGCACATGTTGCGGCGGCTCAAAGCCGATGTGTTC
AAGAACATGCCCTCCAAGACAGAACTAATTGTGCGTGTGGAGCTGAGCCCTATGCAGAAG
AAATACTACAAGTACATCCTCACTCGAAATTTTGAAGCACTCAATGCCCGAGGTGGTGGC
AACCAGGTGTCTCTGCTGAATGTGGTGATGGATCTTAAGAAGTGCTGCAACCATCCATAC
CTCTTCCCTGTGGCTGCAATGGAAGCTCCTAAGATGCCTAATGGCATGTATGATGGCAGT
GCCCTAATCAGAGCATCTGGGAAATTATTGCTGCTGCAGAAAATGCTCAAGAACCTTAAG
GAGGGTGGGCATCGTGTACTCATCTTTTCCCAGATGACCAAGATGCTAGACCTGCTAGAG
GATTTCTTGGAACATGAAGGTTATAAATACGAACGCATCGATGGTGGAATCACTGGGAAC
ATGCGGCAAGAGGCCATTGACCGCTTCAATGCACCGGGTGCTCAGCAGTTCTGCTTCTTG
CTTTCCACTCGAGCTGGGGGCCTTGGAATCAATCTGGCCACTGCTGACACAGTTATTATC
TATGACTCTGACTGGAACCCCCATAATGACATTCAGGCCTTTAGCAGAGCTCACCGGATT
GGGCAAAATAAAAAGGTAATGATCTACCGGTTTGTGACCCGTGCGTCAGTGGAGGAGCGC
ATCACGCAGGTGGCAAAGAAGAAAATGATGCTGACGCATCTAGTGGTGCGGCCTGGGCTG
GGCTCCAAGACTGGATCTATGTCCAAACAGGAGCTTGATGATATCCTCAAATTTGGCACT
GAGGAACTATTCAAGGATGAAGCCACTGATGGAGGAGGAGACAACAAAGAGGGAGAAGAT
AGCAGTGTTATCCACTACGATGATAAGGCCATTGAACGGCTGCTAGACCGTAACCAGGAT
GAGACTGAAGACACAGAATTGCAGGGCATGAATGAATATTTGAGCTCATTCAAAGTGGCC
CAGTATGTGGTACGGGAAGAAGAAATGGGGGAGGAAGAGGAGGTAGAACGGGAAATCATT
AAACAGGAAGAAAGTGTGGATCCTGACTACTGGGAGAAATTGCTGCGGCACCATTATGAG
CAGCAGCAAGAAGATCTAGCCCGAAATCTGGGCAAAGGAAAAAGAATCCGTAAACAGGTC
AACTACAATGATGGCTCCCAGGAGGACCGAGGTGTGTGTGGCCGGCCCCGCCCCCCACCC
ATGGGCCGTTCCACTAGAGCAGTGGGCCCCGCTCATCTGCCCTCTCTCCCTCCAGATTGG
CAGGACGACCAGTCCGACAACCAGTCCGATTACTCAGTGGCTTCAGAGGAAGGTGATGAA
GACTTTGATGAACGTTCAGAAGCTCCCCGTAGGCCCAGTCGTAAGGGCCTGCGGAATGAT
AAAGATAAGCCATTGCCTCCTCTGTTGGCCCGTGTTGGTGGGAATATTGAAGTACTTGGT
TTTAATGCTCGTCAGCGAAAAGCCTTTCTTAATGCAATTATGCGATATGGTATGCCACCT
CAGGATGCTTTTACTACCCAGTGGCTTGTAAGAGACCTGCGAGGCAAATCAGAGAAAGAG
TTCAAGGCATATGTCTCTCTTTTCATGCGGCATTTATGTGAGCCGGGGGCAGATGGGGCT
GAGACCTTTGCTGATGGTGTCCCCCGAGAAGGCCTGTCTCGCCAGCATGTCCTTACTAGA
ATTGGTGTTATGTCTTTGATTCGCAAGAAGGTTCAGGAGTTTGAACATGTTAATGGGCGC
TGGAGCATGCCTGAACTGGCTGAGGTGGAGGAAAACAAGAAGATGTCCCAGCCAGGGTCA
CCCTCCCCAAAAACTCCTACACCCTCCACTCCAGGGGACACGCAGCCCAACACTCCTGCA
CCTGTCCCACCTGCTGAAGATGGGATAAAAATAGAGGAAAATAGCCTCAAAGAAGAAGAG
AGCATAGAAGGAGAAAAGGAGGTTAAATCTACAGCCCCTGAGACTGCCATTGAGTGTACA
CAGGCCCCTGCCCCTGCCTCAGAGGATGAAAAGGTCGTTGTTGAACCCCCTGAGGGAGAG
GAGAAAGTGGAAAAGGCAGAGGTGAAGGAGAGAACAGAGGAACCTATGGAGACAGAGCCC
AAAGGTGCTGCTGATGTAGAGAAGGTGGAGGAAAAGTCAGCAATAGATCTGACCCCTATT
GTGGTAGAAGACAAAGAAGAGAAGAAAGAAGAAGAAGAGAAAAAAGAGGTGATGCTTCAG
AATGGAGAGACCCCCAAGGACCTGAATGATGAGAAACAGAAGAAAAATATTAAACAACGT
TTCATGTTTAACATTGCAGATGGTGGTTTTACTGAGTTGCACTCCCTTTGGCAGAATGAA
GAGCGGGCAGCCACAGTTACCAAGAAGACTTATGAGATCTGGCATCGACGGCATGACTAC
TGGCTGCTAGCCGGCATTATAAACCATGGCTATGCCCGGTGGCAAGACATCCAGAATGAC
CCACGCTATGCCATCCTCAATGAGCCTTTCAAGGGTGAAATGAACCGTGGCAATTTCTTA
GAGATCAAGAATAAATTTCTAGCTCGAAGGTTTAAGCTCTTAGAACAAGCTCTGGTGATT
GAGGAACAGCTGCGCCGGGCTGCTTACTTGAACATGTCAGAAGACCCTTCTCACCCTTCC
ATGGCCCTCAACACCCGCTTTGCTGAGGTGGAGTGTTTGGCGGAAAGTCATCAGCACCTG
TCCAAGGAGTCAATGGCAGGAAACAAGCCAGCCAATGCAGTCCTGCACAAAGTTCTGAAA
CAGCTGGAAGAACTGCTGAGTGACATGAAAGCTGATGTGACTCGACTCCCAGCTACCATT
GCCCGAATTCCCCCAGTTGCTGTGAGGTTACAGATGTCAGAGCGTAACATTCTCAGCCGC
CTGGCAAACCGGGCACCCGAACCTACCCCACAGCAGGTAGCCCAGCAGCAGTTG
Protein sequence predicted based on nucleotide sequence
MASGLGSPSPCSAGSEEEDMDALLNNSLPPPHPEDPEEDLSETETPKLKKKKKPKKPRDP KIPKSKRQKKERMLLCRQLGDSSGEGPEFVEEEEEVALRSDSEGSDYTPGKKKKKKLGPK KEKKSKSKRKEEEEEEDDDDDSKEPKSSAQLLEDWGMEDIDHVFSEEDYRTLTNYKAFSQ FVRPLIAAKNPKIAVSKMMMVLGAKWREFSTNNPFKGSSGASVAAAAAAAVAVVESMVTA TEVAPPPPPVEVPIRKAKTKEGKGPNARRKPKGSPRVPDAKKPKPKKVAPLKIKLGGFGS KRKRSSSEDDDLDVESDFDDASINSYSVSDGSTSRSSRSRKKLRTTKKKKKGEEEVTAVD GYETDHQDYCEVCQQGGEIILCDTCPRAYHMVCLDPDMEKAPEGKWSCPHCEKEGIQWEA KEDNSEGEEILEEVGGDLEEEDDHHMEFCRVCKDGGELLCCDTCPSSYHIHCLNPPLPEI PNGEWLCPRCTCPALKGKVQKILIWKWGQPPSPTPVPRPPDADPNTPSPKPLEGRPERQF FVKWQGMSYWHCSWVSELQLELHCQVMFRNYQRKNDMDEPPSGDFGGDEEKSRKRKNKDP KFAEMEERFYRYGIKPEWMMIHRILNHSVDKKGHVHYLIKWRDLPYDQASWESEDVEIQD YDLFKQSYWNHRELMRGEEGRPGKKLKKVKLRKLERPPETPTVDPTVKYERQPEYLDATG GTLHPYQMEGLNWLRFSWAQGTDTILADEMGLGKTVQTAVFLYSLYKEGHSKGPFLVSAP LSTIINWEREFEMWAPDMYVVTYVGDKDSRAIIRENEFSFEDNAIRGGKKASRMKKEASV KFHVLLTSYELITIDMAILGSIDWACLIVDEAHRLKNNQSKFFRVLNGYSLQHKLLLTGT PLQNNLEELFHLLNFLTPERFHNLEGFLEEFADIAKEDQIKKLHDMLGPHMLRRLKADVF KNMPSKTELIVRVELSPMQKKYYKYILTRNFEALNARGGGNQVSLLNVVMDLKKCCNHPY LFPVAAMEAPKMPNGMYDGSALIRASGKLLLLQKMLKNLKEGGHRVLIFSQMTKMLDLLE DFLEHEGYKYERIDGGITGNMRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVII YDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKKKMMLTHLVVRPGL GSKTGSMSKQELDDILKFGTEELFKDEATDGGGDNKEGEDSSVIHYDDKAIERLLDRNQD ETEDTELQGMNEYLSSFKVAQYVVREEEMGEEEEVEREIIKQEESVDPDYWEKLLRHHYE QQQEDLARNLGKGKRIRKQVNYNDGSQEDRGVCGRPRPPPMGRSTRAVGPAHLPSLPPDW QDDQSDNQSDYSVASEEGDEDFDERSEAPRRPSRKGLRNDKDKPLPPLLARVGGNIEVLG FNARQRKAFLNAIMRYGMPPQDAFTTQWLVRDLRGKSEKEFKAYVSLFMRHLCEPGADGA ETFADGVPREGLSRQHVLTRIGVMSLIRKKVQEFEHVNGRWSMPELAEVEENKKMSQPGS PSPKTPTPSTPGDTQPNTPAPVPPAEDGIKIEENSLKEEESIEGEKEVKSTAPETAIECT QAPAPASEDEKVVVEPPEGEEKVEKAEVKERTEEPMETEPKGAADVEKVEEKSAIDLTPI VVEDKEEKKEEEEKKEVMLQNGETPKDLNDEKQKKNIKQRFMFNIADGGFTELHSLWQNE ERAATVTKKTYEIWHRRHDYWLLAGIINHGYARWQDIQNDPRYAILNEPFKGEMNRGNFL EIKNKFLARRFKLLEQALVIEEQLRRAAYLNMSEDPSHPSMALNTRFAEVECLAESHQHL SKESMAGNKPANAVLHKVLKQLEELLSDMKADVTRLPATIARIPPVAVRLQMSERNILSR LANRAPEPTPQQVAQQQL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
163
End on reference sequence
5976
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
