HSBP1 (H. sapiens )
In pLDNT7_nFLAG (Flag-tagged
In vitro expression vector)

Explanation of Terms

Gene:
Gene Symbol:  HSBP1
Gene Name:  heat shock factor binding protein 1
Original Clone ID:
FLH271225.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format: 
CLOSED
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00300516
Price: 
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 231nts
Open reading frame: 1 to 231

ATGGCCGAGACTGACCCCAAGACCGTGCAGGACCTCACCTCGGTGGTGCAGACACTCCTG
CAGCAGATGCAAGATAAATTTCAGACCATGTCTGACCAGATCATTGGGAGAATTGATGAT
ATGAGTAGTCGCATTGATGATCTGGAAAAGAATATCGCGGACCTCATGACACAGGCTGGG
GTGGAAGAACTGGAAAGTGAAAACAAGATACCTGCCACGCAAAAGAGTTAG

Protein sequence predicted based on nucleotide sequence

MAETDPKTVQDLTSVVQTLLQQMQDKFQTMSDQIIGRIDDMSSRIDDLEKNIADLMTQAG
VEELESENKIPATQKS*

Coding Sequence Details

Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 286
Start on reference sequence 56
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition: Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments: Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable