Gene:
Gene Symbol:
PCDHB16
Gene Name:
protocadherin beta 16
Original Clone ID:
FLH271308.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pLDNT7_nFLAG
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00300988
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGAGATTGGATGGATGCACAATCGGAGACAAAGGCAAGTCCTTGTTTTCTTTGTTTTG
CTGAGCTTGTCTGGGGCGGGCGCCGAGTTGGGGTCCTATTCCGTAGTGGAAGAAACGGAG
AGAGGCTCTTTTGTGGCAAATCTAGGAAAAGACCTGGGGTTGGGGTTGACAGAGATGTCC
ACCCGCAAGGCCAGGATCATTTCCCAGGGGAACAAACAGCATTTGCAGCTCAAGGCTCAA
ACTGGGGATTTGCTCATAAATGAGAAGCTAGATCGAGAGGAGCTATGCGGTCCCACTGAG
CCTTGCATACTACATTTCCAAGTGTTAATGGAAAACCCTTTAGAAATATTTCAGGCTGAA
CTGAGGGTGATAGATATAAATGACCATTCTCCCATGTTCACTGAAAAGGAAATGATTCTA
AAAATACCGGAAAACAGTCCTCTAGGAACTGAGTTCCCTCTGAATCATGCTTTGGACTTG
GACGTAGGAAGCAATAATGTTCAAAACTATAAAATCAGCCCAAGCTCTCATTTCCGGGTT
CTAATCCATGAATTCAGAGATGGCAGGAAATACCCTGAGCTAGTGTTGGATAAAGAGCTG
GATCGGGAGGAGGAGCCTCAACTAAGATTAACCCTGACAGCGCTGGATGGTGGCTCTCCA
CCGCGATCTGGAACTGCTCAGGTCCGTATTGAAGTGGTGGACATCAATGATAACGCTCCT
GAGTTTGAGCAGCCCATCTACAAAGTGCAGATTCCAGAGAACAGTCCTCTTGGCTCCCTG
GTTGCCACCGTCTCCGCCAGGGATTTAGACGGCGGAGCCAATGGAAAAATATCATACACA
CTCTTTCAGCCTTCGGAGGATATTAGTAAAACTTTGGAGGTAAATCCTATGACAGGGGAA
GTTCGACTGAGAAAGCAAGTAGATTTCGAAATGGTTACGTCTTATGAAGTGCGCATCAAA
GCCACAGATGGGGGAGGTCTTTCAGGAAAGTGCACTCTTCTCCTGCAGGTGGTGGACGTG
AATGACAATCCCCCACAGGTGACCATGTCTGCACTCACCAGCCCCATCGCAGAGAACTCG
CCTGAGATAGTAGTTGCTGTTTTCAGCGTTTCAGATCCTGACTCCGGAAACAATGGGAAG
ACGATTTCCTCCATCCAGGAAGACCTTCCCTTTCTTCTAAAACCTTCAGTCAAGAACTTT
TACACCTTGGTAACGGAGAGAGCACTCGACAGAGAAGCAAGAGCTGAATATAATATCACC
CTCACCGTCACAGATATGGGGACTCCAAGGCTGAAAACGGAGCACAACATAACAGTGCAG
ATATCAGATGTCAATGATAACGCCCCCACTTTCACCCAAACCTCCTACACCCTGTTCGTC
CGCGAGAACAACAGCCCCGCCCTGCACATCGGCAGCGTCAGCGCCACAGACAGAGACTCG
GGCACCAACGCCCAGGTCACCTACTCGCTGCTGCCGCCCCAAGACCCGCACCTGCCCCTC
GCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGTTCGCCCTCAGGTCGCTGGAC
TACGAGGCCCTGCAGGCTTTCGAGTTCCGCGTGGGCGCCACAGACCGCGGCTCCCCCGCG
CTGAGCAGAGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTA
GTGCTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACTGAGCTGGTGCCCCGGGCGGCC
GAGCCGGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAATGCC
TGGCTGTCGTACCAGCTGCTCAAGGCCACGGAGCCCGGGCTGTTCGGTGTGTGGGCGCAC
AATGGCGAGGTGCGCACCGCCAGGCTGCTGAGCGAGCGCGACGCAGCCAAGCAGAGGCTG
GTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCACCGCCACGCTGCACGTG
CTCCTGGTGGACGGCTTCTCCCAGCCCTTCCTGCCGCTCCCAGAGGCGGCCCCCGGCCAG
ACCCAGGCCAACTCGCTCACTGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCGTCGCTC
TTCCTCTTTTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCC
TCGGTGGGCCGCTGCTCGATGCCTGAGGGCCCCTTTCCAGGGCGTCTGGTGGACGTAAGC
GGCACCGGGACCCTGTCCCAGAGCTACCAATACGAGGTGTGTCTGACAGGAGGCTCAGAA
ACAAGTGAGTTCAAGTTCCTGAAGCCGATTATCCCCAACTTCTCTCCTTTG
Protein sequence predicted based on nucleotide sequence
MEIGWMHNRRQRQVLVFFVLLSLSGAGAELGSYSVVEETERGSFVANLGKDLGLGLTEMS TRKARIISQGNKQHLQLKAQTGDLLINEKLDREELCGPTEPCILHFQVLMENPLEIFQAE LRVIDINDHSPMFTEKEMILKIPENSPLGTEFPLNHALDLDVGSNNVQNYKISPSSHFRV LIHEFRDGRKYPELVLDKELDREEEPQLRLTLTALDGGSPPRSGTAQVRIEVVDINDNAP EFEQPIYKVQIPENSPLGSLVATVSARDLDGGANGKISYTLFQPSEDISKTLEVNPMTGE VRLRKQVDFEMVTSYEVRIKATDGGGLSGKCTLLLQVVDVNDNPPQVTMSALTSPIAENS PEIVVAVFSVSDPDSGNNGKTISSIQEDLPFLLKPSVKNFYTLVTERALDREARAEYNIT LTVTDMGTPRLKTEHNITVQISDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDS GTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPA LSREALVRVLVLDANDNSPLVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNA WLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKQRLVVLVKDNGEPPRSATATLHV LLVDGFSQPFLPLPEAAPGQTQANSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAA SVGRCSMPEGPFPGRLVDVSGTGTLSQSYQYEVCLTGGSETSEFKFLKPIIPNFSPL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
150
End on reference sequence
2480
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin, 34 ug/mL chloramphenicol
Growth Condition:
Growth with the double antibiotic in LB supplemented with 7% sucrose at 37 degrees is recommended.
Comments:
Conditions for Creator-type vectors in with insert (recombined) form. Selection differs for the unrecombined, empty form of the vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pLDNT7_nFLAG
Synonyms:
pLDNT7-Flag
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Vector for in vitro translation with mammalian extracts, adds N-terminal Flag epitope tag; amp resistance; recombinational cloning
Comments:
None
Size (bp):
4557
Parent Vector:
None
Properties:
Creator, acceptor (destination), in vitro transcription, loxP, ssDNA production, with tag/fusion/marker
Author Name:
Daniel Schiwek
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE1 ori
ColE1 origin of replication
1842
2524
promoter
T7
T7 promoter
4462
4479
recombination site
LoxP
LoxP site for recombinational cloning
657
690
selectable marker
AmpR
ampicillin resistance gene
2622
3281
ssDNA origin
f1 ori
f1 origin for ssDNA production
3928
4368
tag
Flag
3xFlag epitope tag ORF and LoxP region
585
650
trxn termination sequence
T7 term
T7 terminator sequence
1614
1660
