DNASU Plasmid | KIF18A (H. sapiens )<br>In pANT7_cGST (GST-tagged<br>In vitro expression vector)

Explanation of Terms

Gene:

Gene Symbol: KIF18A

Gene Name: kinesin family member 18A

Map: pANT7_cGST.doc

Original Clone ID:

FLH470822.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pANT7_cGST

Format:

FUSION

Source:

HIP

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

HsCD00301942

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 2694nts

Open reading frame: 1 to 2694

Reference Sequence Alignment

ATGTCTGTCACTGAGGAAGACCTGTGCCACCATATGAAAGTAGTAGTTCGTGTACGTCCG
GAAAACACTAAAGAAAAAGCAGCTGGATTTCATAAAGTGGTTCATGTTGTGGATAAACAT
ATCCTAGTTTTTGATCCCAAACAAGAAGAAGTCAGTTTTTTCCATGGAAAGAAAACTACA
AATCAAAATGTTATAAAGAAACAAAATAAGGATCTTAAATTTGTATTTGATGCTGTTTTT
GATGAAACGTCAACTCAGTCAGAAGTTTTTGAACACACTACTAAGCCAATTCTTCGTAGT
TTTTTGAATGGATATAATTGCACAGTACTTGCCTATGGTGCCACTGGTGCTGGGAAGACC
CACACTATGCTAGGATCAGCTGATGAACCTGGAGTGATGTATCTAACAATGTTACACCTT
TACAAATGCATGGATGAGATTAAAGAAGAGAAAATATGTAGTACTGCAGTTTCATATCTG
GAGGTATATAATGAACAGATTCGTGATCTCTTAGTAAATTCAGGGCCACTTGCTGTCCGG
GAAGATACCCAAAAAGGGGTGGTCGTTCATGGACTTACTTTACACCAGCCCAAATCCTCA
GAAGAAATTTTACATTTATTGGATAATGGAAACAAAAACAGGACACAACATCCCACTGAT
ATGAATGCCACATCTTCTCGTTCTCATGCTGTTTTCCAAATTTACTTGCGACAACAAGAC
AAAACAGCAAGTATCAATCAAAATGTCCGTATTGCCAAGATGTCACTCATTGACCTGGCA
GGATCTGAGCGAGCAAGTACTTCCGGTGCTAAGGGGACCCGATTTGTAGAAGGCACAAAT
ATTAATAGATCACTTTTAGCTCTTGGGAATGTCATCAATGCCTTAGCAGATTCAAAGAGA
AAGAATCAGCATATCCCTTACAGAAATAGTAAGCTTACTCGCTTGTTAAAGGATTCTCTT
GGAGGAAACTGTCAAACTATAATGATAGCTGCTGTTAGTCCTTCCTCTGTATTCTACGAT
GACACATATAACACTCTTAAGTATGCTAACCGGGCAAAGGACATTAAATCTTCTTTGAAG
AGCAATGTTCTTAATGTCAATAATCATATAACTCAATATGTAAAGATCTGTAATGAGCAG
AAGGCAGAGATTTTATTGTTAAAAGAAAAACTAAAAGCCTATGAAGAACAGAAAGCCTTC
ACTAATGAAAATGACCAAGCAAAGTTAATGATTTCAAACCCTCAGGAAAAAGAAATCGAA
AGGTTTCAAGAAATCCTGAACTGCTTGTTCCAGAATCGAGAAGAAATTAGACAAGAATAT
CTGAAGTTGGAAATGTTACTTAAAGAAAATGAACTTAAATCATTCTACCAACAACAGTGC
CATAAACAAATAGAAATGATGTGTTCTGAAGACAAAGTAGAAAAGGCCACTGGAAAACGA
GATCATAGACTTGCAATGTTGAAAACTCGTCGCTCCTACCTGGAGAAAAGGAGGGAGGAG
GAATTGAAGCAATTTGATGAGAATACTAATTGGCTCCATCGTGTCGAAAAAGAAATGGGA
CTCTTAAGTCAAAACGGTCATATTCCAAAGGAACTCAAGAAAGATCTTCATTGTCACCAT
TTGCACCTCCAGAACAAAGATTTGAAAGCACAAATTAGACATATGATGGATCTAGCTTGT
CTTCAGGAACAGCAACACAGGCAGACTGAAGCAGTATTGAATGCTTTACTTCCAACCCTA
AGAAAACAATATTGCACATTAAAAGAAGCCGGCCTGTCAAATGCTGCTTTTGAATCTGAC
TTCAAAGAGATCGAACATTTGGTAGAGAGGAAAAAAGTGGTAGTTTGGGCTGACCAAACT
GCCGAACAACCAAAGCAAAACGATCTACCAGGGATTTCTGTTCTTATGACCTTTCCACAA
CTTGGACCAGTTCAGCCTATTCCTTGTTGCTCATCTTCAGGTGGAACTAATCTGGTTAAG
ATTCCTACAGAAAAAAGAACTCGGAGAAAACTAATGCCATCTCCCTTGAAAGGACAGCAT
ACTCTAAAGTCTCCACCATCTCAAAGTGTGCAGCTCAATGATTCTCTTAGCAAAGAACTT
CAGCCTATTGTATATACACCAGAAGACTGTAGAAAAGCTTTTCAAAATCCGTCTACAGTA
ACCTTAATGAAACCATCATCATTTACTACAAGTTTTCAGGCTATCAGCTCAAACATAAAC
AGTGATAATTGTCTGAAAATGTTGTGTGAAGTAGCTATCCCTCATAATAGAAGAAAAGAA
TGTGGACAGGAGGACTTGGACTCTACATTTACTATATGTGAAGACATCAAGAGCTCGAAG
TGTAAATTACCCGAACAAGAATCACTACCAAATGATAACAAAGACATTTTACAACGGCTT
GATCCTTCTTCATTCTCAACTAAGCATTCTATGCCTGTACCAAGCATGGTGCCATCCTAC
ATGGCAATGACTACTGCTGCCAAAAGGAAACGGAAATTAACAAGTTCTACATCAAACAGT
TCGTTAACTGCAGACGTAAATTCTGGATTTGCCAAACGTGTTCGACAAGATAATTCAAGT
GAGAAGCACTTACAAGAAAACAAACCAACAATGGAACATAAAAGAAACATCTGTAAAATA
AATCCAAGCATGGTTAGAAAATTTGGAAGAAATATTTCAAAAGGAAATCTAAGA

Protein sequence predicted based on nucleotide sequence

MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTT
NQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKT
HTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVR
EDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQD
KTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKR
KNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLK
SNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIE
RFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKR
DHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHH
LHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESD
FKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVK
IPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTV
TLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSK
CKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNS
SLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Insert was fully sequenced in parent vector

Reference Sequence Annotations:

Start on reference sequence 130

End on reference sequence 2826

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pANT7_cGST

Synonyms:

pANT7-cGST

Sequencing Primer:

Forward: NAP150

Reverse: NAP138R

Description:

with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial

Comments:

One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems

Size (bp):

5964

Parent Vector:

None

Properties:

Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker

Author Name:

Joshua LaBaer
Al Lau
Niroshan Ramachandran

Publications:

PMID: 15232106

Title: Self-assembling protein microarrays

Vector Map:

Vector Sequence:

Sequence:

GGGCGAATTAATTCCGGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTCTTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTCTGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTGCGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAAAGAGTCAAATGGCTCACCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAAGGTACCCCATTGTATGGGATCTGATCTGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAATATGGATCGGATCCGAATTCGAGCTCCGTCATCAACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGGCGGCCGCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGGATTTTGAGTTAGGATCCGTCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAAAGATCTGGATCCGGCTTACTAAAAGCCAGATAACAGTATGCGTATTTGCGCGCTGATTTTTGCGGTATAAGAATATATACTGATATGTATACCCGAAGTATGTCAAAAAGAGGTGTGCTATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATATGATGTCAATATCTCCGGTCTGGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTGAGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGAAATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCTCCCTTATACACAGCCAGTCTGCAGGTCGACCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGTTGATGACAAGCTTGCGGCCGCACTCGAGCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAATCGGATCTGGTTCCGCGTTGAAGATCTGACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTAAACACTAGTCCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAACGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCGCGCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTGTAATACGACTCACTATA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

ColE

ColE1 bacterial origin of replication

3931

3249

death cassette

ccdB

ccdB death cassette present in 'empty vector' form only (replace with gene of interest)

1876

2085

primer

NAP138

NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?

2405

2384

primer

NAP150

Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?

388

408

promoter

T7 transcriptional start sequence

5940

5964

recombination site

attR1

AttR1 site for recombination in empty vector

549

565

recombination site

attR2

AttR2 site for recombination in empty vector

2236

2251

selectable marker

CmR

chloramphenicol resistance

782

1438

selectable marker

AmpR

ampicillin resistance gene

4688

4029

tag

GST

Glutathione transferase (GST) tag

2282

2950

Gene

Gene Symbol :

KIF18A

Symbol Nomenclature :

KIF18A

SYNONYM :

MS-KIF18A

Designation :

kinesin-like protein KIF18A

Full Nomenclature :

kinesin family member 18A

GENEID :

81930

Locus Tag :

OK/SW-cl.108

HGNC :

29441

MIM :

611271

Vega :

OTTHUMG00000166195

Target GenBank:

AL136819

Reference Sequence Alignment

  

HsCD00301942      ATGTCTGTCACTGAGGAAGACCTGTGCCACCATATGAAAGTAGTAGTTCGTGTACGTCCG
NM_031217.3       ATGTCTGTCACTGAGGAAGACCTGTGCCACCATATGAAAGTAGTAGTTCGTGTACGTCCG
                  ************************************************************

HsCD00301942      GAAAACACTAAAGAAAAAGCAGCTGGATTTCATAAAGTGGTTCATGTTGTGGATAAACAT
NM_031217.3       GAAAACACTAAAGAAAAAGCAGCTGGATTTCATAAAGTGGTTCATGTTGTGGATAAACAT
                  ************************************************************

HsCD00301942      ATCCTAGTTTTTGATCCCAAACAAGAAGAAGTCAGTTTTTTCCATGGAAAGAAAACTACA
NM_031217.3       ATCCTAGTTTTTGATCCCAAACAAGAAGAAGTCAGTTTTTTCCATGGAAAGAAAACTACA
                  ************************************************************

HsCD00301942      AATCAAAATGTTATAAAGAAACAAAATAAGGATCTTAAATTTGTATTTGATGCTGTTTTT
NM_031217.3       AATCAAAATGTTATAAAGAAACAAAATAAGGATCTTAAATTTGTATTTGATGCTGTTTTT
                  ************************************************************

HsCD00301942      GATGAAACGTCAACTCAGTCAGAAGTTTTTGAACACACTACTAAGCCAATTCTTCGTAGT
NM_031217.3       GATGAAACGTCAACTCAGTCAGAAGTTTTTGAACACACTACTAAGCCAATTCTTCGTAGT
                  ************************************************************

HsCD00301942      TTTTTGAATGGATATAATTGCACAGTACTTGCCTATGGTGCCACTGGTGCTGGGAAGACC
NM_031217.3       TTTTTGAATGGATATAATTGCACAGTACTTGCCTATGGTGCCACTGGTGCTGGGAAGACC
                  ************************************************************

HsCD00301942      CACACTATGCTAGGATCAGCTGATGAACCTGGAGTGATGTATCTAACAATGTTACACCTT
NM_031217.3       CACACTATGCTAGGATCAGCTGATGAACCTGGAGTGATGTATCTAACAATGTTACACCTT
                  ************************************************************

HsCD00301942      TACAAATGCATGGATGAGATTAAAGAAGAGAAAATATGTAGTACTGCAGTTTCATATCTG
NM_031217.3       TACAAATGCATGGATGAGATTAAAGAAGAGAAAATATGTAGTACTGCAGTTTCATATCTG
                  ************************************************************

HsCD00301942      GAGGTATATAATGAACAGATTCGTGATCTCTTAGTAAATTCAGGGCCACTTGCTGTCCGG
NM_031217.3       GAGGTATATAATGAACAGATTCGTGATCTCTTAGTAAATTCAGGGCCACTTGCTGTCCGG
                  ************************************************************

HsCD00301942      GAAGATACCCAAAAAGGGGTGGTCGTTCATGGACTTACTTTACACCAGCCCAAATCCTCA
NM_031217.3       GAAGATACCCAAAAAGGGGTGGTCGTTCATGGACTTACTTTACACCAGCCCAAATCCTCA
                  ************************************************************

HsCD00301942      GAAGAAATTTTACATTTATTGGATAATGGAAACAAAAACAGGACACAACATCCCACTGAT
NM_031217.3       GAAGAAATTTTACATTTATTGGATAATGGAAACAAAAACAGGACACAACATCCCACTGAT
                  ************************************************************

HsCD00301942      ATGAATGCCACATCTTCTCGTTCTCATGCTGTTTTCCAAATTTACTTGCGACAACAAGAC
NM_031217.3       ATGAATGCCACATCTTCTCGTTCTCATGCTGTTTTCCAAATTTACTTGCGACAACAAGAC
                  ************************************************************

HsCD00301942      AAAACAGCAAGTATCAATCAAAATGTCCGTATTGCCAAGATGTCACTCATTGACCTGGCA
NM_031217.3       AAAACAGCAAGTATCAATCAAAATGTCCGTATTGCCAAGATGTCACTCATTGACCTGGCA
                  ************************************************************

HsCD00301942      GGATCTGAGCGAGCAAGTACTTCCGGTGCTAAGGGGACCCGATTTGTAGAAGGCACAAAT
NM_031217.3       GGATCTGAGCGAGCAAGTACTTCCGGTGCTAAGGGGACCCGATTTGTAGAAGGCACAAAT
                  ************************************************************

HsCD00301942      ATTAATAGATCACTTTTAGCTCTTGGGAATGTCATCAATGCCTTAGCAGATTCAAAGAGA
NM_031217.3       ATTAATAGATCACTTTTAGCTCTTGGGAATGTCATCAATGCCTTAGCAGATTCAAAGAGA
                  ************************************************************

HsCD00301942      AAGAATCAGCATATCCCTTACAGAAATAGTAAGCTTACTCGCTTGTTAAAGGATTCTCTT
NM_031217.3       AAGAATCAGCATATCCCTTACAGAAATAGTAAGCTTACTCGCTTGTTAAAGGATTCTCTT
                  ************************************************************

HsCD00301942      GGAGGAAACTGTCAAACTATAATGATAGCTGCTGTTAGTCCTTCCTCTGTATTCTACGAT
NM_031217.3       GGAGGAAACTGTCAAACTATAATGATAGCTGCTGTTAGTCCTTCCTCTGTATTCTACGAT
                  ************************************************************

HsCD00301942      GACACATATAACACTCTTAAGTATGCTAACCGGGCAAAGGACATTAAATCTTCTTTGAAG
NM_031217.3       GACACATATAACACTCTTAAGTATGCTAACCGGGCAAAGGACATTAAATCTTCTTTGAAG
                  ************************************************************

HsCD00301942      AGCAATGTTCTTAATGTCAATAATCATATAACTCAATATGTAAAGATCTGTAATGAGCAG
NM_031217.3       AGCAATGTTCTTAATGTCAATAATCATATAACTCAATATGTAAAGATCTGTAATGAGCAG
                  ************************************************************

HsCD00301942      AAGGCAGAGATTTTATTGTTAAAAGAAAAACTAAAAGCCTATGAAGAACAGAAAGCCTTC
NM_031217.3       AAGGCAGAGATTTTATTGTTAAAAGAAAAACTAAAAGCCTATGAAGAACAGAAAGCCTTC
                  ************************************************************

HsCD00301942      ACTAATGAAAATGACCAAGCAAAGTTAATGATTTCAAACCCTCAGGAAAAAGAAATCGAA
NM_031217.3       ACTAATGAAAATGACCAAGCAAAGTTAATGATTTCAAACCCTCAGGAAAAAGAAATCGAA
                  ************************************************************

HsCD00301942      AGGTTTCAAGAAATCCTGAACTGCTTGTTCCAGAATCGAGAAGAAATTAGACAAGAATAT
NM_031217.3       AGGTTTCAAGAAATCCTGAACTGCTTGTTCCAGAATCGAGAAGAAATTAGACAAGAATAT
                  ************************************************************

HsCD00301942      CTGAAGTTGGAAATGTTACTTAAAGAAAATGAACTTAAATCATTCTACCAACAACAGTGC
NM_031217.3       CTGAAGTTGGAAATGTTACTTAAAGAAAATGAACTTAAATCATTCTACCAACAACAGTGC
                  ************************************************************

HsCD00301942      CATAAACAAATAGAAATGATGTGTTCTGAAGACAAAGTAGAAAAGGCCACTGGAAAACGA
NM_031217.3       CATAAACAAATAGAAATGATGTGTTCTGAAGACAAAGTAGAAAAGGCCACTGGAAAACGA
                  ************************************************************

HsCD00301942      GATCATAGACTTGCAATGTTGAAAACTCGTCGCTCCTACCTGGAGAAAAGGAGGGAGGAG
NM_031217.3       GATCATAGACTTGCAATGTTGAAAACTCGTCGCTCCTACCTGGAGAAAAGGAGGGAGGAG
                  ************************************************************

HsCD00301942      GAATTGAAGCAATTTGATGAGAATACTAATTGGCTCCATCGTGTCGAAAAAGAAATGGGA
NM_031217.3       GAATTGAAGCAATTTGATGAGAATACTAATTGGCTCCATCGTGTCGAAAAAGAAATGGGA
                  ************************************************************

HsCD00301942      CTCTTAAGTCAAAACGGTCATATTCCAAAGGAACTCAAGAAAGATCTTCATTGTCACCAT
NM_031217.3       CTCTTAAGTCAAAACGGTCATATTCCAAAGGAACTCAAGAAAGATCTTCATTGTCACCAT
                  ************************************************************

HsCD00301942      TTGCACCTCCAGAACAAAGATTTGAAAGCACAAATTAGACATATGATGGATCTAGCTTGT
NM_031217.3       TTGCACCTCCAGAACAAAGATTTGAAAGCACAAATTAGACATATGATGGATCTAGCTTGT
                  ************************************************************

HsCD00301942      CTTCAGGAACAGCAACACAGGCAGACTGAAGCAGTATTGAATGCTTTACTTCCAACCCTA
NM_031217.3       CTTCAGGAACAGCAACACAGGCAGACTGAAGCAGTATTGAATGCTTTACTTCCAACCCTA
                  ************************************************************

HsCD00301942      AGAAAACAATATTGCACATTAAAAGAAGCCGGCCTGTCAAATGCTGCTTTTGAATCTGAC
NM_031217.3       AGAAAACAATATTGCACATTAAAAGAAGCCGGCCTGTCAAATGCTGCTTTTGAATCTGAC
                  ************************************************************

HsCD00301942      TTCAAAGAGATCGAACATTTGGTAGAGAGGAAAAAAGTGGTAGTTTGGGCTGACCAAACT
NM_031217.3       TTCAAAGAGATCGAACATTTGGTAGAGAGGAAAAAAGTGGTAGTTTGGGCTGACCAAACT
                  ************************************************************

HsCD00301942      GCCGAACAACCAAAGCAAAACGATCTACCAGGGATTTCTGTTCTTATGACCTTTCCACAA
NM_031217.3       GCCGAACAACCAAAGCAAAACGATCTACCAGGGATTTCTGTTCTTATGACCTTTCCACAA
                  ************************************************************

HsCD00301942      CTTGGACCAGTTCAGCCTATTCCTTGTTGCTCATCTTCAGGTGGAACTAATCTGGTTAAG
NM_031217.3       CTTGGACCAGTTCAGCCTATTCCTTGTTGCTCATCTTCAGGTGGAACTAATCTGGTTAAG
                  ************************************************************

HsCD00301942      ATTCCTACAGAAAAAAGAACTCGGAGAAAACTAATGCCATCTCCCTTGAAAGGACAGCAT
NM_031217.3       ATTCCTACAGAAAAAAGAACTCGGAGAAAACTAATGCCATCTCCCTTGAAAGGACAGCAT
                  ************************************************************

HsCD00301942      ACTCTAAAGTCTCCACCATCTCAAAGTGTGCAGCTCAATGATTCTCTTAGCAAAGAACTT
NM_031217.3       ACTCTAAAGTCTCCACCATCTCAAAGTGTGCAGCTCAATGATTCTCTTAGCAAAGAACTT
                  ************************************************************

HsCD00301942      CAGCCTATTGTATATACACCAGAAGACTGTAGAAAAGCTTTTCAAAATCCGTCTACAGTA
NM_031217.3       CAGCCTATTGTATATACACCAGAAGACTGTAGAAAAGCTTTTCAAAATCCGTCTACAGTA
                  ************************************************************

HsCD00301942      ACCTTAATGAAACCATCATCATTTACTACAAGTTTTCAGGCTATCAGCTCAAACATAAAC
NM_031217.3       ACCTTAATGAAACCATCATCATTTACTACAAGTTTTCAGGCTATCAGCTCAAACATAAAC
                  ************************************************************

HsCD00301942      AGTGATAATTGTCTGAAAATGTTGTGTGAAGTAGCTATCCCTCATAATAGAAGAAAAGAA
NM_031217.3       AGTGATAATTGTCTGAAAATGTTGTGTGAAGTAGCTATCCCTCATAATAGAAGAAAAGAA
                  ************************************************************

HsCD00301942      TGTGGACAGGAGGACTTGGACTCTACATTTACTATATGTGAAGACATCAAGAGCTCGAAG
NM_031217.3       TGTGGACAGGAGGACTTGGACTCTACATTTACTATATGTGAAGACATCAAGAGCTCGAAG
                  ************************************************************

HsCD00301942      TGTAAATTACCCGAACAAGAATCACTACCAAATGATAACAAAGACATTTTACAACGGCTT
NM_031217.3       TGTAAATTACCCGAACAAGAATCACTACCAAATGATAACAAAGACATTTTACAACGGCTT
                  ************************************************************

HsCD00301942      GATCCTTCTTCATTCTCAACTAAGCATTCTATGCCTGTACCAAGCATGGTGCCATCCTAC
NM_031217.3       GATCCTTCTTCATTCTCAACTAAGCATTCTATGCCTGTACCAAGCATGGTGCCATCCTAC
                  ************************************************************

HsCD00301942      ATGGCAATGACTACTGCTGCCAAAAGGAAACGGAAATTAACAAGTTCTACATCAAACAGT
NM_031217.3       ATGGCAATGACTACTGCTGCCAAAAGGAAACGGAAATTAACAAGTTCTACATCAAACAGT
                  ************************************************************

HsCD00301942      TCGTTAACTGCAGACGTAAATTCTGGATTTGCCAAACGTGTTCGACAAGATAATTCAAGT
NM_031217.3       TCGTTAACTGCAGACGTAAATTCTGGATTTGCCAAACGTGTTCGACAAGATAATTCAAGT
                  ************************************************************

HsCD00301942      GAGAAGCACTTACAAGAAAACAAACCAACAATGGAACATAAAAGAAACATCTGTAAAATA
NM_031217.3       GAGAAGCACTTACAAGAAAACAAACCAACAATGGAACATAAAAGAAACATCTGTAAAATA
                  ************************************************************

HsCD00301942      AATCCAAGCATGGTTAGAAAATTTGGAAGAAATATTTCAAAAGGAAATCTAAGA---
NM_031217.3       AATCCAAGCATGGTTAGAAAATTTGGAAGAAATATTTCAAAAGGAAATCTAAGATAA
                  ******************************************************

Pathways

Reactome :

Adaptive Immune System

Reactome :

Cell Cycle

Reactome :

Cell Cycle, Mitotic

Reactome :

Factors involved in megakaryocyte development and platelet production

Reactome :

Hemostasis

Reactome :

Immune System

Reactome :

Kinesins

Reactome :

M Phase

Reactome :

MHC class II antigen presentation

Reactome :

Mitotic Anaphase

Reactome :

Mitotic M-M/G1 phases

Reactome :

Mitotic Metaphase and Anaphase

Reactome :

Mitotic Prometaphase

Reactome :

Resolution of Sister Chromatid Cohesion

Reactome :

Separation of Sister Chromatids

Protein

SMART domain :

KISc : Kinesin motor, catalytic domain. ATPase.

UniProt :

Q8NI77

HPRD :

11177

Clone

Cloning Information :

470822

HIP Master Clone ID :

198742

Original Clone ID :

FLH470822.01L

Organism

Species Specific ID:

81930

Genome

Chromosome :

Map Location :

11p14.1

Ensembl :

ENSG00000121621

Reagents

Labome :

KIF18A-antibody

KIF18A (H. sapiens )In pANT7_cGST (GST-taggedIn vitro expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Organism

Genome

Reagents

KIF18A (H. sapiens )
In pANT7_cGST (GST-tagged
In vitro expression vector)