Gene:
Gene Symbol:
PCDH11X
Gene Name:
protocadherin 11 X-linked
Original Clone ID:
FLH469781.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00302550
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGTTTAGGGTTGGCTTCTTAATAATTTCTTCTTCCTCTTCTCTCTCTCCTCTTCTTTTG
GTCAGTGTTGTGCGGGTTAATACAACAAACTGTCACAAGTGTTTGTTGTCCGGGACGTAC
ATTTTCGCGGTCCTGCTAGTATGCGTGGTGTTCCACTCTGGCGCCCAGGAGAAAAACTAC
ACCATCCGAGAAGAAATTCCAGAAAACGTCCTGATAGGCAACTTGTTGAAAGACCTTAAC
TTGTCGCTGATTCCAAACAAGTCCTTGACAACTACTATGCAGTTCAAGCTAGTGTACAAG
ACCGGAGATGTGCCACTGATTCGAATTGAAGAGGATACTGGTGAGATCTTCACTACCGGC
GCTCGCATTGATCGTGAGAAATTATGTGCTGGTATCCCAAGGGATGAGCATTGCTTTTAT
GAAGTGGAGGTTGCCATTTTGCCGGATGAAATATTTAGACTGGTTAAGATACGTTTTCTG
ATAGAAGATATAAATGATAATGCACCATTGTTCCCAGCAACAGTTATCAACATATCAATT
CCAGAGAACTCGGCTATAAACTCTAAATATACTCTCCCAGCGGCTGTTGATCCTGACGTA
GGCATAAACGGAGTTCAAAACTACGAACTAATTAAGAGTCAAAACATTTTTGGCCTCGAT
GTCATTGAAACACCAGAAGGAGACAAGATGCCACAACTGATTGTTCAAAAGGAGTTAGAT
AGGGAAGAGAAGGATACCTATGTGATGAAAGTAAAGGTTGAAGATGGTGGCTTTCCTCAA
AGATCCAGTACTGCTATTTTGCAAGTAAGTGTTACTGATACAAATGACAACCACCCAGTC
TTTAAGGAGACAGAGATTGAAGTCAGTATACCAGAAAATGCTCCTGTAGGCACTTCAGTG
ACACAGCTCCATGCCACAGATGCTGACATAGGTGAAAATGCCAAGATCCACTTCTCTTTC
AGCAATCTAGTCTCCAACATTGCCAGGAGATTATTTCACCTCAATGCCACCACTGGACTT
ATCACAATCAAAGAACCACTGGATAGGGAAGAAACACCAAACCACAAGTTACTGGTTTTG
GCAAGTGATGGTGGATTGATGCCAGCAAGAGCAATGGTGCTGGTAAATGTTACAGATGTC
AATGATAATGTCCCATCCATTGACATAAGATACATCGTCAATCCTGTCAATGACACAGTT
GTTCTTTCAGAAAATATTCCACTCAACACCAAAATTGCTCTCATAACTGTGACGGATAAG
GATGCGGACCATAATGGCAGGGTGACATGCTTCACAGATCATGAAATTCCTTTCAGATTA
AGGCCAGTATTCAGTAATCAGTTCCTCCTGGAGAATGCAGCATATCTTGACTATGAGTCC
ACAAAAGAATATGCCATTAAATTACTGGCTGCAGATGCTGGCAAACCTCCTTTGAATCAG
TCAGCAATGCTCTTCATCAAAGTGAAAGATGAAAATGACAATGCTCCAGTTTTCACCCAG
TCTTTCGTAACTGTTTCTATTCCTGAGAATAACTCTCCTGGCATCCAGTTGATGAAAGTA
AGTGCAACGGATGCAGACAGTGGGCCTAATGCTGAGATCAATTACCTGCTAGGCCCTGAT
GCTCCACCTGAATTCAGCCTGGATCGTCGTACAGGCATGCTGACTGTAGTGAAGAAACTA
GATAGAGAAAAAGAGGATAAATATTTATTCACAATTCTGGCAAAAGATAATGGGGTACCA
CCCTTAACCAGCAATGTCACAGTCTTTGTAAGCATTATTGATCAGAATGACAATAGCCCA
GTTTTCACTCACAATGAATACAAATTCTATGTCCCAGAAAACCTTCCAAGGCATGGTACA
GTAGGACTAATCACTGTAACTGATCCTGATTATGGAGACAATTCTGCAGTTACGCTCTCC
ATTTTAGATGAGAATGATGACTTCACCATTGATTCACAAACTGGTGTCATCCGACCAAAT
ATTTCATTTGATAGAGAAAAACAAGAATCTTACACTTTCTATGTAAAGGCTGAGGATGGT
GGTAGAGTATCACGTTCTTCAAGTGCCAAAGTAACCATAAATGTGGTTGATGTCAATGAC
AACAAACCAGTTTTCATTGTCCCTCCTTACAACTATTCTTATGAATTGGTTCTACCGTCC
ACTAATCCAGGCACAGTGGTCTTTCAGGTAATTGCTGTTGACAATGACACTGGCATGAAT
GCAGAGGTTCGTTACAGCATTGTAGGAGGAAACACAAGAGATCTGTTTGCAATCGACCAA
GAAACAGGCAACATAACATTGATGGAGAAATGTGATGTTACAGACCTTGGTTTACACAGA
GTGTTGGTCAAAGCTAATGACTTAGGACAGCCTGATTCTCTCTTCAGTGTTGTAATTGTC
AATCTGTTCGTGAATGAGTCAGTGACCAATGCTACACTGATTAATGAACTGGTGCGCAAA
AGCATTGAAGCACCAGTGACCCCAAATACTGAGATAGCTGATGTATCCTCACCAACTAGT
GACTATGTCAAGATCCTGGTTGCAGCTGTTGCTGGCACCATAACTGTCGTTGTAGTTATT
TTCATCACTGCTGTAGTAAGATGTCGCCAGGCACCACACCTTAAGGCTGCTCAGAAAAAC
ATGCAGAATTCTGAATGGGCTACCCCAAACCCAGAAAACAGGCAGATGATAATGATGAAG
AAAAAGAAAAAGAAGAAGAAGCATTCCCCTAAGAACCTGCTGCTTAATGTTGTCACTATT
GAAGAAACTAAGGCAGATGATGTTGACAGTGATGGAAACAGAGTCACACTAGACCTTCCT
ATTGATCTAGAAGAGCAAACAATGGGAAAGTACAATTGGGTAACTACACCTACTACTTTC
AAGCCTGACAGCCCTGATTTGGCCCGACACTACAAATCTGCCTCTCCACAGCCTGCCTTC
CAAATTCAGCCTGAAACTCCCCTGAATTTGAAGCACCACATCATCCAAGAACTGCCTCTC
GATAACACCTTTGTGGCCTGTGACTCTATCTCCAATTGTTCCTCAAGCAGTTCAGATCCC
TACAGCGTTTCTGACTGTGGCTATCCAGTGACAACCTTCGAGGTACCTGTGTCCGTACAC
ACCAGACCGTCCCAGCGGCGTGTCACATTTCACCTGCCAGAAGGCTCTCAGGAAAGCAGC
AGTGATGGTGGACTGGGAGACCATGATGCAGGCAGCCTTACCAGCACATCCCATGGCCTG
CCCCTTGGCTATCCTCAGGAGGAGTACTTTGATCGTGCTACACCCAGCAATCGCACTGAA
GGGGATGGCAACTCCGATCCTGAATCTACTTTCATACCTGGACTAAAGAAAGAAATAACT
GTTCAACCAACTGTGGAAGAGGCCTCTGACAACTGCACTCAAGAATGTCTCATCTATGGC
CATTCTGATGCCTGCTGGATGCCGGCATCTCTGGATCATTCCAGCTCTTCACAAGCACAG
GCCTCTGCTCTATGCCACAGCCCACCACTGTCACAGGCCTCTACTCAGCACCACAGCCCA
CCAGTGACACAGACCATTGTTCTCTGCCACAGCCCTCCAGTGACACAGACCATCGCATTG
TGCCACAGCCCACCACCGATACAGGTGTCTGCTCTCCACCACAGTCCTCCTCTAGTGCAG
GGTACTGCACTTCACCACAGCCCACCATCAGCACAGGCCTCAGCCCTCTGCTACAGCCCT
CCTTTAGCACAGGCTGCTGCAATCAGCCACAGCTCTTCTCTGCCACAGGTTATTGCCCTC
CATCGTAGTCAGGCCCAATCATCAGTCAGTTTGCAGCAAGGTTGGGTGCAAGGTGCTAAT
GGACTATGCTCTGTTGATCAGGGAGTGCAAGGTAGTGCAACATCTCAGTTTTACACCATG
TCTGAAAGACTTCATCCCAGTGATGATTCAATTAAAGTCATTCCTTTGACAACCTTCGCT
CCACGCCAACAGGCCAGACCGTCCAGAGGTGATTCCCCCATTATGGAAACACATCCCTTG
Protein sequence predicted based on nucleotide sequence
MFRVGFLIISSSSSLSPLLLVSVVRVNTTNCHKCLLSGTYIFAVLLVCVVFHSGAQEKNY TIREEIPENVLIGNLLKDLNLSLIPNKSLTTTMQFKLVYKTGDVPLIRIEEDTGEIFTTG ARIDREKLCAGIPRDEHCFYEVEVAILPDEIFRLVKIRFLIEDINDNAPLFPATVINISI PENSAINSKYTLPAAVDPDVGINGVQNYELIKSQNIFGLDVIETPEGDKMPQLIVQKELD REEKDTYVMKVKVEDGGFPQRSSTAILQVSVTDTNDNHPVFKETEIEVSIPENAPVGTSV TQLHATDADIGENAKIHFSFSNLVSNIARRLFHLNATTGLITIKEPLDREETPNHKLLVL ASDGGLMPARAMVLVNVTDVNDNVPSIDIRYIVNPVNDTVVLSENIPLNTKIALITVTDK DADHNGRVTCFTDHEIPFRLRPVFSNQFLLENAAYLDYESTKEYAIKLLAADAGKPPLNQ SAMLFIKVKDENDNAPVFTQSFVTVSIPENNSPGIQLMKVSATDADSGPNAEINYLLGPD APPEFSLDRRTGMLTVVKKLDREKEDKYLFTILAKDNGVPPLTSNVTVFVSIIDQNDNSP VFTHNEYKFYVPENLPRHGTVGLITVTDPDYGDNSAVTLSILDENDDFTIDSQTGVIRPN ISFDREKQESYTFYVKAEDGGRVSRSSSAKVTINVVDVNDNKPVFIVPPYNYSYELVLPS TNPGTVVFQVIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVTDLGLHR VLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSIEAPVTPNTEIADVSSPTS DYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNMQNSEWATPNPENRQMIMMK KKKKKKKHSPKNLLLNVVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKYNWVTTPTTF KPDSPDLARHYKSASPQPAFQIQPETPLNLKHHIIQELPLDNTFVACDSISNCSSSSSDP YSVSDCGYPVTTFEVPVSVHTRPSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGL PLGYPQEEYFDRATPSNRTEGDGNSDPESTFIPGLKKEITVQPTVEEASDNCTQECLIYG HSDACWMPASLDHSSSSQAQASALCHSPPLSQASTQHHSPPVTQTIVLCHSPPVTQTIAL CHSPPPIQVSALHHSPPLVQGTALHHSPPSAQASALCYSPPLAQAAAISHSSSLPQVIAL HRSQAQSSVSLQQGWVQGANGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFA PRQQARPSRGDSPIMETHPL
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence
4757
Start on reference sequence
735
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
