Gene:
Gene Symbol:
ARID2
Gene Name:
AT rich interactive domain 2 (ARID, RFX-like)
Original Clone ID:
FLH470575.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00303066
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCAAACTCGACGGGGAAGGCGCCTCCGGACGAGCGGAGAAAGGGACTCGCTTTCCTG
GACGAGCTGCGGCAGTTCCACCACAGCAGAGGGTCGCCTTTTAAAAAAATCCCTGCGGTG
GGTGGGAAGGAGCTGGATCTTCACGGTCTCTACACCAGAGTCACTACTTTAGGCGGATTC
GCGAAGGTTTCTGAGAAGAATCAGTGGGGAGAAATTGTTGAAGAGTTCAACTTTCCCAGA
AGTTGTTCTAACGCTGCCTTTGCTTTAAAACAGTATTACTTGCGTTACCTAGAAAAGTAC
GAGAAAGTTCATCATTTTGGGGAGGATGATGATGAGGTACCACCAGGCAATCCAAAGCCA
CAGCTTCCTATTGGTGCAATTCCATCTTCCTACAATTACCAGCAACACAGTGTGTCGGAT
TATCTGCGTCAAAGTTATGGGCTGTCCATGGACTTTAATTCGCCAAATGATTATAATAAA
TTGGTGCTTTCACTGTTATCTGGACTCCCAAATGAAGTGGACTTTGCTATTAACGTATGC
ACTCTCCTATCAAATGAAAGCAAGCACGTCATGCAACTTGAAAAAGATCCTAAAATCATC
ACTTTACTACTTGCTAATGCCGGGGTGTTTGACGACACTTTAGGATCCTTTTCCACTGTA
TTTGGAGAAGAATGGAAAGAGAAGACTGATAGAGACTTCGTTAAGTTTTGGAAAGACATC
GTTGATGATAATGAAGTTCGTGACCTCATTTCTGACAGAAACAAGTCTCATGAAGGTACA
TCAGGAGAATGGATTTGGGAGTCTTTATTTCATCCACCTCGAAAGCTGGGCATTAACGAT
ATTGAAGGACAGCGGGTACTTCAGATTGCAGTGATTTTGAGAAATCTTTCCTTTGAGGAG
GGCAATGTTAAGCTCTTGGCAGCTAATCGTACCTGTCTTCGTTTCCTATTACTTTCTGCA
CATAGTCATTTTATTTCTTTAAGGCAATTAGGCCTTGACACATTAGGAAATATTGCAGCT
GAGCTTTTACTGGACCCTGTTGATTTCAAAACTACTCATCTGATGTTTCATACTGTTACA
AAATGTCTAATGTCAAGGGATAGATTTTTAAAGATGAGAGGCATGGAAATTTTGGGAAAT
CTTTGCAAAGCAGAAGATAATGGTGTTTTAATTTGTGAATATGTGGATCAGGATTCCTAC
AGAGAGATCATTTGTCATCTCACTTTACCTGATGTGCTGCTTGTAATCTCAACACTCGAG
GTGCTATACATGCTCACGGAAATGGGAGATGTTGCTTGCACAAAAATTGCAAAAGTAGAA
AAGAGCATAGACATGTTAGTGTGTCTGGTTTCTATGGATATTCAGATGTTTGGCCCTGAT
GCACTAGCTGCGGTAAAACTCATTGAACACCCAAGTTCCAGTCATCAAATGTTATCTGAA
ATTAGGCCACAAGCTATAGAGCAAGTCCAAACCCAGACTCATGTAGCATCTGCCCCAGCT
TCCAGAGCAGTTGTAGCGCAGCATGTTGCTCCACCTCCAGGAATAGTGGAAATAGATAGT
GAGAAGTTTGCTTGTCAGTGGCTAAATGCTCATTTTGAAGTAAATCCAGATTGTTCTGTT
TCTCGAGCAGAAATGTATTCTGAATACCTCTCGACTTGCAGTAAATTAGCTCGTGGTGGA
ATCCTAACATCAACTGGATTTTATAAATGTCTTAGAACGGTCTTTCCAAATCATACAGTG
AAGAGAGTGGAGGATTCCAGTAGCAATGGGCAGGCACATATTCATGTGGTAGGAGTAAAA
CGGAGGGCTATACCACTTCCCATTCAGATGTACTATCAGCAGCAACCAGTTTCTACTTCT
GTTGTTCGTGTTGATTCTGTTCCTGATGTATCTCCTGCTCCTTCACCTGCAGGAATCCCT
CATGGATCACAAACCATAGGAAACCATTTTCAGAGGACTCCTGTTGCCAACCAATCTTCA
AATCTGACTGCAACACAAATGTCTTTTCCTGTACAAGGTGTTCATACTGTGGCACAAACT
GTTTCAAGAATTCCACAAAATCCTTCACCTCATACCCACCAGCAACAAAATGCTCCAGTG
ACTGTCATTCAAAGTAAAGCTCCAATTCCTTGTGAAGTTGTTAAGGCTACAGTTATCCAG
AATTCCATACCCCAGACAGGAGTTCCTGTTAGTATTGCTGTTGGAGGAGGACCTCCACAG
AGTTCTGTTGTTCAGAATCATAGTACAGGGCCACAACCTGTTACAGTTGTGAATTCTCAG
ACATTGCTTCACCATCCATCTGTAATTCCACAGCAGTCTCCATTACACACAGTGGTACCA
GGACAGATCCCTTCAGGCACTCCTGTTACAGTAATTCAACAAGCTGTCCCACAGAGTCAT
ATGTTTGGCAGAGTACAGAACATACCAGCATGTACTTCTACAGTTTCACAGGGTCAACAG
TTAATCACCACATCACCCCAACCTGTGCAAACTTCATCTCAACAGACATCAGCTGGTAGC
CAGTCACAAGATACTGTTATCATAGCACCCCCACAGTATGTAACAACTTCTGCATCCAAT
ATTGTCTCAGCAACTTCAGTACAGAATTTTCAGGTAGCTACAGGACAAATGGTTACTATT
GCTGGTGTCCCAAGTCCACAAGCCTCAAGGGTAGGGTTTCAGAACATTGCACCAAAACCT
CTCCCTTCTCAGCAAGTTTCATCTACAGTGGTACAGCAGCCTATTCAACAACCACAGCAG
CCAACCCAACAAAGCGTAGTGATTGTAAGCCAGCCAGCTCAACAAGGTCAAACTTATGCA
CCAGCCATTCACCAAATTGTTCTTGCTAATCCAGCAGCTCTTCCAGCTGGTCAGACAGTT
CAGCTAACTGGACAACCTAACATAACTCCATCTTCTTCACCATCACCTGTCCCAGCTACT
AATAACCAAGTCCCTACTGCCATGTCGTCGTCCTCTACCCCTCAATCACAGGGACCACCT
CCTACTGTCAGTCAAATGTTATCTGTGAAAAGGCAGCAACAGCAGCAACATTCACCAGCA
CCCCCACCACAGCAGGTACAAGTACAAGTTCAGCAGCCCCAACAAGTACAGATGCAAGTT
CAACCTCAACAGTCGAATGCAGGAGTTGGTCAGCCTGCCTCTGGTGAGTCGAGTCTGATT
AAACAGCTTCTGCTTCCGAAACGTGGTCCTTCAACACCAGGTGGTAAGCTTATTCTCCCA
GCTCCACAGATTCCTCCCCCTAATAATGCAAGAGCTCCTAGCCCTCAGGTGGTCTATCAG
GTGGCCAGTAACCAAGCCGCAGGTTTTGGAGTGCAGGGGCAAACTCCAGCTCAGCAGCTA
TTGGTTGGGCAGCAAAATGTTCAGTTGGTCCCAAGTGCAATGCCACCCTCAGGGGGAGTA
CAAACTGTGCCCATTTCGAACTTACAAATATTGCCAGGTCCACTGATCTCAAATAGCCCA
GCAACCATTTTCCAAGGGACTTCTGGCAACCAGGTAACCATAACAGTTGTGCCAAATACG
AGTTTTGCACCTGCAACTGTGAGTCAGGGAAATGCAACTCAGCTCATTGCTCCAGCAGGA
ATTACCATGAGCGGAACGCAGACAGGAGTTGGACTTCCAGTACAAACGCTTCCAGCCACT
CAAGCATCTCCTGCTGGACAATCATCATGTACTACTGCTACTCCCCCATTCAAAGGTGAT
AAAATAATTTGCCAAAAGGAGGAGGAAGCAAAGGAAGCAACAGGTTTACATGTTCATGAA
CGTAAAATTGAAGTCATGGAGAACCCGTCCTGCCGACGAGGAGCCACAAACACCAGCAAT
GGGGATACAAAGGAAAATGAAATGCATGTGGGAAGTCTTTTAAATGGGAGAAAGTACAGT
GACTCAAGTCTACCTCCTTCAAACTCAGGGAAAATTCAAAGTGAGACTAATCAGTGCTCA
CTAATCAGTAATGGGCCATCATTGGAATTAGGTGAGAATGGAGCATCTGGGAAACAGAAC
TCAGAACAAATAGACATGCAAGATATCAAAAGTGATTTGAGAAAACCGCTAGTTAATGGA
ATCTGTGATTTTGATAAAGGAGATGGTTCTCATTTAAGCAAAAACATTCCAAATCATAAA
ACTTCCAATCATGTAGGAAATGGTGAGATATCTCCAATGGAACCACAAGGGACTTTAGAT
ATCACTCAGCAAGATACTGCCAAAGGTGATCAACTAGAAAGAATTTCTAATGGACCTGTA
TTAACTTTGGGTGGTTCATCTGTGAGCAGTATACAGGAGGCTTCAAATGCGGCAACACAG
CAATTTAGTGGTACTGATTTGCTTAATGGACCTCTAGCTTCAAGTTTGAATTCAGATGTG
CCTCAGCAACGCCCAAGTGTAGTTGTCTCACCACATTCTACAACCTCTGTTATACAGGGA
CATCAAATCATAGCAGTTCCCGACTCAGGATCAAAAGTATCCCATTCTCCTGCCCTATCA
TCTGACGTTCGGTCTACAAATGGCACAGCAGAATGCAAAACTGTAAAGAGGCCAGCAGAG
GATACTGATAGGGAAACAGTCGCAGGAATTCCAAATAAAGTAGGAGTTAGAATTGTTACA
ATCAGTGACCCCAACAATGCTGGCTGCAGCGCAACAATGGTTGCTGTGCCAGCAGGAGCA
GATCCAAGCACTGTAGCTAAAGTAGCAATAGAAAGTGCTGTTCAGCAAAAGCAACAGCAT
CCACCAACATATGTACAGAATGTGGTCCCGCAGAACACTCCTATGCCACCTTCACCAGCT
GTACAAGTGCAGGGCCAGCCTAACAGTTCTCAGCCTTCTCCATTCAGTGGATCCAGTCAG
CCTGGAGATCCAATGAGAAAACCTGGACAGAACTTCATGTGTCTGTGGCAGTCTTGTAAA
AAGTGGTTTCAGACACCCTCACAGGTTTTCTACCATGCAGCAACTGAACATGGAGGAAAA
GATGTATATCCAGGGCAGTGTCTTTGGGAAGGTTGTGAGCCTTTTCAGCGACAGCGGTTT
TCTTTTATTACCCACTTGCAGGATAAGCACTGTTCAAAGGATGCCCTACTTGCAGGATTA
AAACAAGATGAACCAGGACAAGCAGGAAGTCAGAAGTCTTCTACCAAGCAGCCAACTGTA
GGGGGCACAAGCTCAACTCCTAGAGCACAAAAGGCCATTGTGAATCATCCCAGTGCTGCA
CTTATGGCTCTGAGGAGAGGATCAAGAAACCTTGTCTTTCGAGATTTTACAGATGAAAAA
GAGGGACCAATAACTAAACACATCCGACTAACAGCTGCCTTAATATTAAAAAATATTGGT
AAATATTCAGAATGTGGTCGCAGATTGTTAAAGAGACATGAAAATAACTTATCAGTGCTA
GCCATTAGTAACATGGAAGCTTCCTCCACCCTTGCCAAATGCCTTTATGAACTTAATTTT
ACAGTTCAGAGTAAGGAACAAGAAAAAGACTCAGAAATGCTGCAG
Protein sequence predicted based on nucleotide sequence
MANSTGKAPPDERRKGLAFLDELRQFHHSRGSPFKKIPAVGGKELDLHGLYTRVTTLGGF AKVSEKNQWGEIVEEFNFPRSCSNAAFALKQYYLRYLEKYEKVHHFGEDDDEVPPGNPKP QLPIGAIPSSYNYQQHSVSDYLRQSYGLSMDFNSPNDYNKLVLSLLSGLPNEVDFAINVC TLLSNESKHVMQLEKDPKIITLLLANAGVFDDTLGSFSTVFGEEWKEKTDRDFVKFWKDI VDDNEVRDLISDRNKSHEGTSGEWIWESLFHPPRKLGINDIEGQRVLQIAVILRNLSFEE GNVKLLAANRTCLRFLLLSAHSHFISLRQLGLDTLGNIAAELLLDPVDFKTTHLMFHTVT KCLMSRDRFLKMRGMEILGNLCKAEDNGVLICEYVDQDSYREIICHLTLPDVLLVISTLE VLYMLTEMGDVACTKIAKVEKSIDMLVCLVSMDIQMFGPDALAAVKLIEHPSSSHQMLSE IRPQAIEQVQTQTHVASAPASRAVVAQHVAPPPGIVEIDSEKFACQWLNAHFEVNPDCSV SRAEMYSEYLSTCSKLARGGILTSTGFYKCLRTVFPNHTVKRVEDSSSNGQAHIHVVGVK RRAIPLPIQMYYQQQPVSTSVVRVDSVPDVSPAPSPAGIPHGSQTIGNHFQRTPVANQSS NLTATQMSFPVQGVHTVAQTVSRIPQNPSPHTHQQQNAPVTVIQSKAPIPCEVVKATVIQ NSIPQTGVPVSIAVGGGPPQSSVVQNHSTGPQPVTVVNSQTLLHHPSVIPQQSPLHTVVP GQIPSGTPVTVIQQAVPQSHMFGRVQNIPACTSTVSQGQQLITTSPQPVQTSSQQTSAGS QSQDTVIIAPPQYVTTSASNIVSATSVQNFQVATGQMVTIAGVPSPQASRVGFQNIAPKP LPSQQVSSTVVQQPIQQPQQPTQQSVVIVSQPAQQGQTYAPAIHQIVLANPAALPAGQTV QLTGQPNITPSSSPSPVPATNNQVPTAMSSSSTPQSQGPPPTVSQMLSVKRQQQQQHSPA PPPQQVQVQVQQPQQVQMQVQPQQSNAGVGQPASGESSLIKQLLLPKRGPSTPGGKLILP APQIPPPNNARAPSPQVVYQVASNQAAGFGVQGQTPAQQLLVGQQNVQLVPSAMPPSGGV QTVPISNLQILPGPLISNSPATIFQGTSGNQVTITVVPNTSFAPATVSQGNATQLIAPAG ITMSGTQTGVGLPVQTLPATQASPAGQSSCTTATPPFKGDKIICQKEEEAKEATGLHVHE RKIEVMENPSCRRGATNTSNGDTKENEMHVGSLLNGRKYSDSSLPPSNSGKIQSETNQCS LISNGPSLELGENGASGKQNSEQIDMQDIKSDLRKPLVNGICDFDKGDGSHLSKNIPNHK TSNHVGNGEISPMEPQGTLDITQQDTAKGDQLERISNGPVLTLGGSSVSSIQEASNAATQ QFSGTDLLNGPLASSLNSDVPQQRPSVVVSPHSTTSVIQGHQIIAVPDSGSKVSHSPALS SDVRSTNGTAECKTVKRPAEDTDRETVAGIPNKVGVRIVTISDPNNAGCSATMVAVPAGA DPSTVAKVAIESAVQQKQQHPPTYVQNVVPQNTPMPPSPAVQVQGQPNSSQPSPFSGSSQ PGDPMRKPGQNFMCLWQSCKKWFQTPSQVFYHAATEHGGKDVYPGQCLWEGCEPFQRQRF SFITHLQDKHCSKDALLAGLKQDEPGQAGSQKSSTKQPTVGGTSSTPRAQKAIVNHPSAA LMALRRGSRNLVFRDFTDEKEGPITKHIRLTAALILKNIGKYSECGRRLLKRHENNLSVL AISNMEASSTLAKCLYELNFTVQSKEQEKDSEMLQ
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
5508
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
