Gene:
Gene Symbol:
ADAMTS14
Gene Name:
ADAM metallopeptidase with thrombospondin type 1 motif, 14
Original Clone ID:
FLH469848.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pANT7_cGST
Format:
FUSION
Source:
HIP
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
HsCD00303117
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGGCTCCACTCCGCGCGCTGCTGTCCTACCTGCTGCCTTTGCACTGTGCGCTCTGCGCC
GCCGCGGGCAGCCGGACCCCAGAGCTGCACCTCTCTGGAAAGCTCAGTGACTATGGTGTG
ACAGTGCCCTGCAGCACAGACTTTCGGGGACGCTTCCTCTCCCACGTGGTGTCTGGCCCA
GCAGCAGCCTCTGCAGGGAGCATGGTAGTGGACACGCCACCCACACTACCACGACACTCC
AGTCACCTCCGGGTGGCTCGCAGCCCTCTGCACCCAGGAGGGACCCTGTGGCCTGGCAGG
GTGGGGCGCCACTCCCTCTACTTCAATGTCACTGTTTTCGGGAAGGAACTGCACTTGCGC
CTGCGGCCCAATCGGAGGTTGGTAGTGCCAGGATCCTCAGTGGAGTGGCAGGAGGATTTT
CGGGAGCTGTTCCGGCAGCCCTTACGGCAGGAGTGTGTGTACACTGGAGGTGTCACTGGA
ATGCCTGGGGCAGCTGTTGCCATCAGCAACTGTGACGGATTGGCGGGCCTCATCCGCACA
GACAGCACCGACTTCTTCATTGAGCCTCTGGAGCGGGGCCAGCAGGAGAAGGAGGCCAGC
GGGAGGACACATGTGGTGTACCGCCGGGAGGCCGTCCAGCAGGAGTGGGCAGAACCTGAC
GGGGACCTGCACAATGAAGCCTTTGGCCTGGGAGACCTTCCCAACCTGCTGGGCCTGGTG
GGGGACCAGCTGGGCGACACAGAGCGGAAGCGGCGGCATGCCAAGCCAGGCAGCTACAGC
ATCGAGGTGCTGCTGGTGGTGGACGACTCGGTGGTTCGCTTCCATGGCAAGGAGCATGTG
CAGAACTATGTCCTCACCCTCATGAATATCGTAGATGAGATTTACCACGATGAGTCCCTG
GGGGTTCATATAAATATTGCCCTCGTCCGCTTGATCATGGTTGGCTACCGACAGTCCCTG
AGCCTGATCGAGCGCGGGAACCCCTCACGCAGCCTGGAGCAGGTGTGTCGCTGGGCACAC
TCCCAGCAGCGCCAGGACCCCAGCCACGCTGAGCACCATGACCACGTTGTGTTCCTCACC
CGGCAGGACTTTGGGCCCTCAGGTATGCAAGGGTATGCACCCGTCACTGGCATGTGTCAC
CCCCTGAGGAGCTGTGCCCTCAACCATGAGGATGGCTTCTCCTCAGCCTTCGTGATAGCT
CATGAGACCGGCCACGTGCTCGGCATGGAGCATGACGGTCAGGGGAATGGCTGTGCAGAT
GAGACCAGCCTGGGCAGCGTCATGGCGCCCCTGGTGCAGGCTGCCTTCCACCGCTTCCAT
TGGTCCCGCTGCAGCAAGCTGGAGCTCAGCCGCTACCTCCCCTCCTACGACTGCCTCCTC
GATGACCCCTTTGATCCTGCCTGGCCCCAGCCCCCAGAGCTGCCTGGGATCAACTACTCA
ATGGATGAGCAGTGCCGCTTTGACTTTGGCAGTGGCTACCAGACCTGCTTGGCATTCAGG
ACCTTTGAGCCCTGCAAGCAGCTGTGGTGCAGCCATCCTGACAACCCGTACTTCTGCAAG
ACCAAGAAGGGGCCCCCGCTGGATGGGACTGAGTGTGCACCCGGCAAGTGGTGCTTCAAA
GGTCACTGCATCTGGAAGTCGCCGGAGCAGACATATGGCCAGGATGGAGGCTGGAGCTCC
TGGACCAAGTTTGGGTCATGTTCGCGGTCATGTGGGGGCGGGGTGCGATCCCGCAGCCGG
AGCTGCAACAACCCCTCCCCAGCCTATGGAGGCCGCCTGTGCTTAGGGCCCATGTTCGAG
TACCAGGTCTGCAACAGCGAGGAGTGCCCTGGGACCTACGAGGACTTCCGGGCCCAGCAG
TGTGCCAAGCGCAACTCCTACTATGTGCACCAGAATGCCAAGCACAGCTGGGTGCCCTAC
GAGCCTGACGATGACGCCCAGAAGTGTGAGCTGATCTGCCAGTCGGCGGACACGGGGGAC
GTGGTGTTCATGAACCAGGTGGTTCACGATGGGACACGCTGCAGCTACCGGGACCCATAC
AGCGTCTGTGCGCGTGGCGAGTGTGTGCCTGTCGGCTGTGACAAGGAGGTGGGGTCCATG
AAGGCGGATGACAAGTGTGGAGTCTGCGGGGGTGACAACTCCCACTGCAGGACTGTGAAG
GGGACGCTGGGCAAGGCCTCCAAGCAGGCAGGAGCTCTCAAGCTGGTGCAGATCCCAGCA
GGTGCCAGGCACATCCAGATTGAGGCACTGGAGAAGTCCCCCCACCGCATTGTGGTGAAG
AACCAGGTCACCGGCAGCTTCATCCTCAACCCCAAGGGCAAGGAAGCCACAAGCCGGACC
TTCACCGCCATGGGCCTGGAGTGGGAGGATGCGGTGGAGGATGCCAAGGAAAGCCTCAAG
ACCAGCGGGCCCCTGCCTGAAGCCATTGCCATCCTGGCTCTCCCCCCAACTGAGGGTGGC
CCCCGCAGCAGCCTGGCCTACAAGTACGTCATCCATGAGGACCTGCTGCCCCTTATCGGG
AGCAACAATGTGCTCCTGGAGGAGATGGACACCTATGAGTGGGCGCTCAAGAGCTGGGCC
CCCTGCAGCAAGGCCTGTGGAGGAGGGATCCAGTTCACCAAATACGGCTGCCGGCGCAGA
CGAGACCACCACATGGTGCAGCGACACCTGTGTGACCACAAGAAGAGGCCCAAGCCCATC
CGCCGGCGCTGCAACCAGCACCCGTGCTCTCAGCCTGTGTGGGTGACGGAGGAGTGGGGT
GCCTGCAGCCGGAGCTGTGGGAAGCTGGGGGTGCAGACACGGGGGATACAGTGCCTGCTG
CCCCTCTCCAATGGAACCCACAAGGTCATGCCGGCCAAAGCCTGCGCCGGGGACCGGCCT
GAGGCCCGACGGCCCTGTCTCCGAGTGCCCTGCCCAGCCCAGTGGAGGCTGGGAGCCTGG
TCCCAGTGCTCTGCCACCTGTGGAGAGGGCATCCAGCAGCGGCAGGTGGTGTGCAGGACC
AACGCCAACAGCCTCGGGCATTGCGAGGGGGATAGGCCAGACACTGTCCAGGTCTGCAGC
CTGCCCGCCTGTGGAGGAAATCACCAGAACTCCACGGTGAGGGCCGATGTCTGGGAACTT
GGGACGCCAGAGGGGCAGTGGGTGCCACAATCTGAACCCCTACATCCCATTAACAAGATA
TCATCAACGGAGCCCTGCACGGGAGACAGGTCTGTCTTCTGCCAGATGGAAGTGCTCGAT
CGCTACTGCTCCATTCCCGGCTACCACCGGCTCTGCTGTGTGTCCTGCATCAAGAAGGCC
TCGGGCCCCAACCCTGGCCCAGACCCTGGCCCAACCTCACTGCCCCCCTTCTCCACTCCT
GGAAGCCCCTTACCAGGACCCCAGGACCCTGCAGATGCTGCAGAGCCTCCTGGAAAGCCA
ACGGGATCAGAGGACCATCAGCATGGCCGAGCCACACAGCTCCCAGGAGCTCTGGATACA
AGCTCCCCAGGGACCCAGCATCCCTTTGCCCCTGAGACACCAATCCCTGGAGCATCCTGG
AGCATCTCCCCTACCACCCCCGGGGGGCTGCCTTGGGGCTGGACTCAGACACCTACGCCA
GTCCCTGAGGACAAAGGGCAACCTGGAGAAGACCTGAGACATCCCGGCACCAGCCTCCCT
GCTGCCTCCCCGGTGACA
Protein sequence predicted based on nucleotide sequence
MAPLRALLSYLLPLHCALCAAAGSRTPELHLSGKLSDYGVTVPCSTDFRGRFLSHVVSGP AAASAGSMVVDTPPTLPRHSSHLRVARSPLHPGGTLWPGRVGRHSLYFNVTVFGKELHLR LRPNRRLVVPGSSVEWQEDFRELFRQPLRQECVYTGGVTGMPGAAVAISNCDGLAGLIRT DSTDFFIEPLERGQQEKEASGRTHVVYRREAVQQEWAEPDGDLHNEAFGLGDLPNLLGLV GDQLGDTERKRRHAKPGSYSIEVLLVVDDSVVRFHGKEHVQNYVLTLMNIVDEIYHDESL GVHINIALVRLIMVGYRQSLSLIERGNPSRSLEQVCRWAHSQQRQDPSHAEHHDHVVFLT RQDFGPSGMQGYAPVTGMCHPLRSCALNHEDGFSSAFVIAHETGHVLGMEHDGQGNGCAD ETSLGSVMAPLVQAAFHRFHWSRCSKLELSRYLPSYDCLLDDPFDPAWPQPPELPGINYS MDEQCRFDFGSGYQTCLAFRTFEPCKQLWCSHPDNPYFCKTKKGPPLDGTECAPGKWCFK GHCIWKSPEQTYGQDGGWSSWTKFGSCSRSCGGGVRSRSRSCNNPSPAYGGRLCLGPMFE YQVCNSEECPGTYEDFRAQQCAKRNSYYVHQNAKHSWVPYEPDDDAQKCELICQSADTGD VVFMNQVVHDGTRCSYRDPYSVCARGECVPVGCDKEVGSMKADDKCGVCGGDNSHCRTVK GTLGKASKQAGALKLVQIPAGARHIQIEALEKSPHRIVVKNQVTGSFILNPKGKEATSRT FTAMGLEWEDAVEDAKESLKTSGPLPEAIAILALPPTEGGPRSSLAYKYVIHEDLLPLIG SNNVLLEEMDTYEWALKSWAPCSKACGGGIQFTKYGCRRRRDHHMVQRHLCDHKKRPKPI RRRCNQHPCSQPVWVTEEWGACSRSCGKLGVQTRGIQCLLPLSNGTHKVMPAKACAGDRP EARRPCLRVPCPAQWRLGAWSQCSATCGEGIQQRQVVCRTNANSLGHCEGDRPDTVQVCS LPACGGNHQNSTVRADVWELGTPEGQWVPQSEPLHPINKISSTEPCTGDRSVFCQMEVLD RYCSIPGYHRLCCVSCIKKASGPNPGPDPGPTSLPPFSTPGSPLPGPQDPADAAEPPGKP TGSEDHQHGRATQLPGALDTSSPGTQHPFAPETPIPGASWSISPTTPGGLPWGWTQTPTP VPEDKGQPGEDLRHPGTSLPAASPVT
Coding Sequence Details
Insert Sequence Verified?:
N
Verification Method:
Insert was fully sequenced in parent vector
Reference Sequence Annotations:
Start on reference sequence
1
End on reference sequence
3681
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pANT7_cGST
Synonyms:
pANT7-cGST
Description:
with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments:
One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp):
5964
Parent Vector:
None
Properties:
Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name:
Joshua LaBaer
Al Lau
Niroshan Ramachandran
Al Lau
Niroshan Ramachandran
Publications:
PMID:
15232106
Title: Self-assembling protein microarrays
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin of replication
3931
3249
death cassette
ccdB
ccdB death cassette present in 'empty vector' form only (replace with gene of interest)
1876
2085
primer
NAP138
NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3?
2405
2384
primer
NAP150
Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3?
388
408
promoter
T7
T7 transcriptional start sequence
5940
5964
recombination site
attR1
AttR1 site for recombination in empty vector
549
565
recombination site
attR2
AttR2 site for recombination in empty vector
2236
2251
selectable marker
CmR
chloramphenicol resistance
782
1438
selectable marker
AmpR
ampicillin resistance gene
4688
4029
tag
GST
Glutathione transferase (GST) tag
2282
2950
