Gene:
Gene Symbol:
BAI3
Gene Name:
brain-specific angiogenesis inhibitor 3
Original Clone ID:
FLH272027.01L
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pDONR201
Format:
FUSION
Source:
BMS
Description:
None
Comments:
Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.
Mutation / Discrepancy:
Unknown / Unknown
Publications:
None
Authors:
Bristol-Myers Squibb
HsCD00304930
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
ATGAAGGCTGTTCGTAACCTGCTGATTTATATATTTTCCACCTATCTCCTGGTTATGTTT
GGATTTAATGCTGCCCAAGACTTCTGGTGTTCAACTTTGGTGAAGGGAGTCATTTATGGA
TCGTATTCTGTAAGTGAAATGTTTCCTAAAAACTTTACAAACTGCACTTGGACGCTGGAA
AATCCAGATCCAACCAAATATAGCATTTACCTGAAATTTTCCAAAAAGGACCTTAGCTGC
TCTAACTTTTCACTCCTGGCTTATCAGTTTGATCATTTTTCCCATGAAAAAATAAAGGAT
CTTTTAAGAAAGAATCATTCTATAATGCAACTCTGCAATTCCAAGAATGCTTTCGTTTTT
CTACAGTATGATAAAAATTTTATTCAAATACGTCGAGTATTTCCAACTAATTTCCCAGGA
TTACAGAAAAAAGGGGAAGAAGATCAGAAATCTTTTTTTGAGTTTTTGGTATTGAACAAG
GTCAGCCCAAGCCAGTTTGGTTGCCATGTATTATGTACTTGGTTGGAGAGCTGCTTAAAA
TCAGAAAATGGGAGAACAGAATCATGTGGGATCATGTATACAAAATGCACCTGCCCTCAG
CATTTGGGAGAGTGGGGGATCGACGACCAGTCGCTGATTTTGTTAAATAACGTGGTGTTA
CCCCTGAATGAGCAGACAGAGGGCTGCCTGACCCAGGAGCTGCAAACCACCCAAGTCTGC
AATCTTACCAGGGAGGCCAAGCGACCACCCAAAGAAGAATTTGGAATGATGGGAGATCAT
ACAATTAAAAGTCAGCGACCTCGATCTGTTCATGAAAAAAGGGTCCCTCAGGAACAAGCT
GATGCTGCTAAATTTATGGCACAAACTGGTGAATCTGGTGTGGAAGAGTGGTCCCAGTGG
AGCACATGTTCGGTTACTTGTGGTCAAGGGTCGCAGGTGCGAACCAGAACTTGTGTATCA
CCTTACGGGACACACTGCAGCGGCCCATTAAGAGAATCAAGGGTTTGCAATAACACTGCC
CTCTGTCCAGTACACGGAGTATGGGAGGAATGGTCACCATGGAGTTTATGTTCATTTACA
TGTGGTCGAGGCCAAAGAACAAGAACAAGGTCATGCACACCTCCTCAGTATGGAGGAAGG
CCGTGTGAAGGACCTGAAACACATCATAAGCCTTGTAATATTGCTCTTTGCCCAGTTGAT
GGACAGTGGCAAGAGTGGAGTTCGTGGAGCCAGTGCTCAGTAACGTGCTCGAATGGGACT
CAGCAGAGAAGCCGGCAGTGCACTGCAGCTGCCCATGGAGGCTCCGAATGCAGAGGGCCA
TGGGCAGAAAGCAGAGAGTGCTATAACCCTGAATGTACAGCCAATGGTCAATGGAATCAG
TGGGGTCATTGGAGTGGTTGTTCCAAGTCCTGTGATGGCGGCTGGGAAAGGCGAATAAGG
ACCTGTCAGGGTGCAGTGATAACAGGGCAGCAATGTGAAGGAACGGGCGAAGAAGTGAGA
AGATGCAGTGAGCAGCGATGCCCTGCACCTTATGAAATATGCCCTGAGGATTATCTGATG
TCGATGGTGTGGAAAAGAACTCCAGCAGGCGACTTGGCATTCAATCAATGTCCCCTGAAT
GCCACAGGCACCACTAGCAGACGCTGCTCTCTCAGTCTTCATGGAGTGGCCTTCTGGGAA
CAGCCGAGCTTTGCAAGATGCATATCAAATGAGTACAGACACTTGCAGCATTCAATTAAA
GAGCACCTTGCTAAGGGGCAGCGAATGCTGGCAGGTGATGGAATGTCCCAGGTGACCAAG
ACACTGTTGGATTTAACTCAGAGAAAAAATTTCTATGCAGGCGATCTTCTGATGTCTGTG
GAGATCCTGAGAAATGTGACAGACACATTTAAAAGGGCAAGTTACATCCCTGCATCTGAT
GGTGTCCAGAACTTCTTTCAAATAGTTAGCAACCTTCTAGATGAAGAAAACAAGGAAAAA
TGGGAAGATGCACAACAGATTTATCCAGGGTCAATAGAGTTAATGCAGGTGATTGAAGAT
TTTATACACATTGTTGGAATGGGGATGATGGACTTTCAGAATTCATACTTAATGACTGGA
AATGTAGTGGCTAGTATTCAGAAGCTTCCTGCAGCCTCTGTTCTAACAGACATCAACTTT
CCAATGAAAGGACGGAAGGGAATGGTTGACTGGGCAAGAAACTCAGAAGATAGGGTAGTA
ATTCCAAAAAGCATTTTCACTCCGGTGTCATCAAAAGAATTAGATGAATCATCTGTATTT
GTTCTTGGCGCAGTCCTATACAAAAACTTAGATCTAATTTTGCCCACTTTGAGAAATTAT
ACTGTCATTAATTCCAAAATCATCGTGGTCACAATAAGGCCTGAACCCAAAACAACCGAT
TCGTTTCTGGAGATAGAACTAGCTCATTTGGCTAATGGTACTTTGAATCCCTATTGTGTA
TTGTGGGATGACTCCAAAACGAACGAGTCTTTGGGAACGTGGTCCACCCAGGGATGTAAA
ACTGTGCTTACCGATGCATCCCATACGAAATGCTTATGTGATCGTCTCTCTACCTTCGCC
ATTTTGGCTCAGCAACCTAGAGAAATAATCATGGAATCCTCTGGCACACCTTCAGTTACC
CTAATAGTAGGCAGTGGTCTTTCTTGCTTGGCCTTGATTACCCTAGCAGTTGTCTATGCA
GCATTATGGAGGTACATACGCTCTGAGAGATCCATAATACTAATTAACTTCTGCCTGTCT
ATCATCTCATCCAATATCCTCATACTGGTTGGACAGACTCAGACACATAATAAGAGTATC
TGCACAACCACCACTGCATTTTTGCACTTTTTCTTCCTGGCTTCATTCTGTTGGGTTTTG
ACTGAGGCGTGGCAATCATATATGGCTGTAACTGGAAAAATTAGGACACGGCTTATAAGA
AAACGCTTTTTGTGCCTTGGATGGGGTTTACCAGCATTAGTAGTGGCCACATCAGTAGGC
TTCACCAGAACAAAAGGATATGGCACTGATCACTACTGCTGGCTCTCTCTTGAAGGAGGA
CTACTCTATGCTTTTGTGGGACCTGCAGCCGCTGTTGTCCTGGTCAACATGGTGATTGGC
ATTTTGGTATTTAATAAACTTGTTTCCAGAGATGGAATCCTAGATAAAAAGCTCAAACAC
AGAGCCGGTCAGATGAGTGAGCCTCATAGCGGTTTGACGCTCAAATGTGCCAAGTGTGGA
GTAGTTTCAACAACAGCTTTGTCAGCCACCACCGCCAGTAACGCCATGGCGTCTCTTTGG
AGCTCCTGTGTGGTGTTGCCCCTTCTGGCTTTGACGTGGATGTCTGCGGTTCTGGCCATG
ACAGATAAACGCTCCATATTGTTTCAAATACTTTTTGCTGTGTTTGATTCATTGCAAGGC
TTTGTTATAGTCATGGTCCACTGCATTCTTCGGAGAGAGGTTCAGGATGCATTTAGATGC
CGATTGAGAAACTGTCAGGATCCCATCAATGCAGATTCTTCGAGTTCGTTTCCTAATGGG
CATGCTCAAATCATGACAGACTTTGAAAAGGATGTAGACATTGCCTGTCGATCAGTTCTT
CATAAGGATATTGGTCCTTGCCGAGCAGCCACAATAACAGGAACACTTTCTAGGATTTCT
CTAAATGATGATGAAGAAGAAAAGGGAACAAACCCTGAAGGGCTAAGCTATTCAACATTG
CCTGGAAATGTCATTTCCAAAGTCATCATCCAGCAACCCACAGGTTTGCACATGCCCATG
AGTATGAATGAGCTTAGCAATCCATGTTTGAAAAAAGAAAATAGTGAATTGCGGAGAACT
GTGTACTTATGTACGGATGATAATTTGAGAGGGGCTGACATGGACATAGTCCATCCTCAA
GAAAGAATGATGGAAAGTGACTATATTGTGATGCCCAGAAGTTCTGTAAATAACCAGCCT
TCAATGAAAGAAGAAAGCAAAATGAATATTGGCATGGAAACCTTGCCACATGAAAGGCTA
TTGCACTACAAAGTAAACCCTGAATTCAATATGAATCCCCCTGTAATGGACCAGTTCAAT
ATGAACTTAGAGCAACATCTCGCACCCCAGGAACATATGCAGAATTTGCCCTTTGAACCT
CGCACAGCTGTGAAGAATTTCATGGCCTCTGAGTTGGATGATAATGCAGGACTATCAAGA
AGTGAAACTGGATCAACGATATCAATGAGTTCTTTAGAGAGAAGAAAATCACGATATTCA
GACCTTGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACTATTTCAAGAA
CTAAATCAGAAATTTCAAACTTTGGACAGATTTCGGGATATACCAAATACAAGCAGTATG
GAAAACCCCGCACCAAACAAGAATCCATGGGACACTTTCAAAAACCCCAGTGAATACCCG
CATTACACCACAATCAATGTCTTAGACACAGAGGCAAAGGATGCTTTGGAACTGAGGCCA
GCAGAGTGGGAGAAGTGTCTGAATTTGCCTCTGGATGTGCAAGAGGGTGACTTTCAAACA
GAAGTTTAA
Protein sequence predicted based on nucleotide sequence
MKAVRNLLIYIFSTYLLVMFGFNAAQDFWCSTLVKGVIYGSYSVSEMFPKNFTNCTWTLE NPDPTKYSIYLKFSKKDLSCSNFSLLAYQFDHFSHEKIKDLLRKNHSIMQLCNSKNAFVF LQYDKNFIQIRRVFPTNFPGLQKKGEEDQKSFFEFLVLNKVSPSQFGCHVLCTWLESCLK SENGRTESCGIMYTKCTCPQHLGEWGIDDQSLILLNNVVLPLNEQTEGCLTQELQTTQVC NLTREAKRPPKEEFGMMGDHTIKSQRPRSVHEKRVPQEQADAAKFMAQTGESGVEEWSQW STCSVTCGQGSQVRTRTCVSPYGTHCSGPLRESRVCNNTALCPVHGVWEEWSPWSLCSFT CGRGQRTRTRSCTPPQYGGRPCEGPETHHKPCNIALCPVDGQWQEWSSWSQCSVTCSNGT QQRSRQCTAAAHGGSECRGPWAESRECYNPECTANGQWNQWGHWSGCSKSCDGGWERRIR TCQGAVITGQQCEGTGEEVRRCSEQRCPAPYEICPEDYLMSMVWKRTPAGDLAFNQCPLN ATGTTSRRCSLSLHGVAFWEQPSFARCISNEYRHLQHSIKEHLAKGQRMLAGDGMSQVTK TLLDLTQRKNFYAGDLLMSVEILRNVTDTFKRASYIPASDGVQNFFQIVSNLLDEENKEK WEDAQQIYPGSIELMQVIEDFIHIVGMGMMDFQNSYLMTGNVVASIQKLPAASVLTDINF PMKGRKGMVDWARNSEDRVVIPKSIFTPVSSKELDESSVFVLGAVLYKNLDLILPTLRNY TVINSKIIVVTIRPEPKTTDSFLEIELAHLANGTLNPYCVLWDDSKTNESLGTWSTQGCK TVLTDASHTKCLCDRLSTFAILAQQPREIIMESSGTPSVTLIVGSGLSCLALITLAVVYA ALWRYIRSERSIILINFCLSIISSNILILVGQTQTHNKSICTTTTAFLHFFFLASFCWVL TEAWQSYMAVTGKIRTRLIRKRFLCLGWGLPALVVATSVGFTRTKGYGTDHYCWLSLEGG LLYAFVGPAAAVVLVNMVIGILVFNKLVSRDGILDKKLKHRAGQMSEPHSGLTLKCAKCG VVSTTALSATTASNAMASLWSSCVVLPLLALTWMSAVLAMTDKRSILFQILFAVFDSLQG FVIVMVHCILRREVQDAFRCRLRNCQDPINADSSSSFPNGHAQIMTDFEKDVDIACRSVL HKDIGPCRAATITGTLSRISLNDDEEEKGTNPEGLSYSTLPGNVISKVIIQQPTGLHMPM SMNELSNPCLKKENSELRRTVYLCTDDNLRGADMDIVHPQERMMESDYIVMPRSSVNNQP SMKEESKMNIGMETLPHERLLHYKVNPEFNMNPPVMDQFNMNLEQHLAPQEHMQNLPFEP RTAVKNFMASELDDNAGLSRSETGSTISMSSLERRKSRYSDLDFEKVMHTRKRHMELFQE LNQKFQTLDRFRDIPNTSSMENPAPNKNPWDTFKNPSEYPHYTTINVLDTEAKDALELRP AEWEKCLNLPLDVQEGDFQTEV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Reference Sequence Annotations:
Start on reference sequence
34
End on reference sequence
4602
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR201
Synonyms:
''
Sequencing Primer:
Forward:
pDONR201-forward
Reverse:
pDONR201-reverse
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;
Comments:
The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp):
4470
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
959
1264
CDS
CmR
confers resistance to chloramphenicol
1606
2265
CDS
KanR
confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes
2868
3677
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
73
100
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
192
278
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2266
2368
protein binding site
attP1
recombination site for the Gateway BP reaction
332
563
protein binding site
attP2
recombination site for the Gateway BP reaction
2513
2744
recombination site
attL1
attL recombination site 1 present in recombined (with insert) form only, position is approximate
332
563
recombination site
attL2
attL recombination site 2 present in recombined (with insert) form only, position is approximate
2744
2513
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
3820
4408
