DNASU Plasmid | CDK2 (Homo sapiens) in pDONR201 (Gateway donor/master vector)

Explanation of Terms

Gene:

Gene Symbol: CDK2

Gene Name: cyclin-dependent kinase 2

Map: pDONR cofig.pdf

Original Clone ID:

FLH272185.01L

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pDONR201

Format:

FUSION

Source:

HIP

Description:

None

Comments:

Clone insert was fully sequence-verified by the clone submitter but the sequence is not available.

Mutation / Discrepancy:

Unknown / Unknown

Publications:

None

Authors:

HIP

HsCD00305088

Price:

AVAILABLE

No restriction
Special MTA: None

Insert Sequence
Protein Sequence

Insert sequence: 897nts

Open reading frame: 1 to 897

ATGGAGAACTTCCAAAAGGTGGAAAAGATCGGAGAGGGCACGTACGGAGTTGTGTACAAA
GCCAGAAACAAGTTGACGGGAGAGGTGGTGGCGCTTAAGAAAATCCGCCTGGACACTGAG
ACTGAGGGTGTGCCCAGTACTGCCATCCGAGAGATCTCTCTGCTTAAGGAGCTTAACCAT
CCTAATATTGTCAAGCTGCTGGATGTCATTCACACAGAAAATAAACTCTACCTGGTTTTT
GAATTTCTGCACCAAGATCTCAAGAAATTCATGGATGCCTCTGCTCTCACTGGCATTCCT
CTTCCCCTCATCAAGAGCTATCTGTTCCAGCTGCTCCAGGGCCTAGCTTTCTGCCATTCT
CATCGGGTCCTCCACCGAGACCTTAAACCTCAGAATCTGCTTATTAACACAGAGGGGGCC
ATCAAGCTAGCAGACTTTGGACTAGCCAGAGCTTTTGGAGTCCCTGTTCGTACTTACACC
CATGAGGTGGTGACCCTGTGGTACCGAGCTCCTGAAATCCTCCTGGGCTGCAAATATTAT
TCCACAGCTGTGGACATCTGGAGCCTGGGCTGCATCTTTGCTGAGATGGTGACTCGCCGG
GCCCTATTCCCTGGAGATTCTGAGATTGACCAGCTCTTCCGGATCTTTCGGACTCTGGGG
ACCCCAGATGAGGTGGTGTGGCCAGGAGTTACTTCTATGCCTGATTACAAGCCAAGTTTC
CCCAAGTGGGCCCGGCAAGATTTTAGTAAAGTTGTACCTCCCCTGGATGAAGATGGACGG
AGCTTGTTATCGCAAATGCTGCACTACGACCCTAACAAGCGGATTTCGGCCAAGGCAGCC
CTGGCTCACCCTTTCTTCCAGGATGTGACCAAGCCAGTACCCCATCTTCGACTCTGA

Protein sequence predicted based on nucleotide sequence

MENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNH
PNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHS
HRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYY
STAVDIWSLGCIFAEMVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSF
PKWARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPVPHLRL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Reference Sequence Annotations:

End on reference sequence 1087

Start on reference sequence 191

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

None

Recommended expression in:

Not Applicable

Vector Name:

pDONR201

Synonyms:

Sequencing Primer:

Forward: pDONR201-forward

Reverse: pDONR201-reverse

Description:

Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with attL1 and attL2 and rrnB T2 and rrnB T1 and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and KanR and ori; 50ug/mL of Kanamycin resistance in Pichia;

Comments:

The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.

Size (bp):

4470

Parent Vector:

None

Properties:

Gateway, bacterial expression, donor (entry), recombinational cloning

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

CTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAAGTTTGTACAAAAAAGCAGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATGAATCAACTACTTAGATGGTATTAGTGACCTGTAGTCGACCGACAGCCTTCCAAATGTTCTTCGGGTGATGCTGCCAACTTAGTCGACCGACAGCCTTCCAAATGTTCTTCTCAAACGGAATCGTCGTATCCAGCCTACTCGCTATTGTCCTCAATGCCGTATTAAATCATAAAAAGAAATAAGAAAAAGAGGTGCGAGCCTCTTTTTTGTGTGACAAAATAAAAACATCTACCTATTCATATACGCTAGTGTCATAGTCCTGAAAATCATCTGCATCAAGAACAATTTCACAACTCTTATACTTTTCTCTTACAAGTCGTTCGGCTTCATCTGGATTTTCAGCCTCTATACTTACTAAACGTGATAAAGTTTCTGTAATTTCTACTGTATCGACCTGCAGACTGGCTGTGTATAAGGGAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGCCGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCGCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTGCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAGCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCTTTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAAATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATCCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCAGGGCTTCCCGGTATCAACAGGGACACCAGGATTTATTTATTCTGCGAAGTGATCTTCCGTCACAGGTATTTATTCGGCGCAAAGTGCGTCGGGTGATGCTGCCAACTTAGTCGACTACAGGTCACTAATACCATCTAAGTAGTTGATTCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGGCATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGCTTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAACTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAATCAGAATTGGTTAATTGGTTGTAACACTGGCAGAGCATTACGCTGACTTGACGGGACGGCGCAAGCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTT

Vector Features:

Type

Name

Description

Start

End

CDS

ccdB

CcdB, a bacterial toxin that poisons DNA gyrase

959

1264

CDS

CmR

confers resistance to chloramphenicol

1606

2265

CDS

KanR

confers resistance to kanamycin in bacteria or G418 (Geneticin) in eukaryotes

2868

3677

Terminator

rrnB T2

transcription terminator T2 from the E. coli rrnB gene

100

Terminator

rrnB T1

transcription terminator T1 from the E. coli rrnB gene

192

278

promoter

cat_promoter-var002

promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase

2266

2368

protein binding site

attP1

recombination site for the Gateway BP reaction

332

563

protein binding site

attP2

recombination site for the Gateway BP reaction

2513

2744

recombination site

attL1

attL recombination site 1 present in recombined (with insert) form only, position is approximate

332

563

recombination site

attL2

attL recombination site 2 present in recombined (with insert) form only, position is approximate

2744

2513

rep_origin

ori

high-copy-number ColE1/pMB1/pBR322/pUC origin of replication

3820

4408

Gene

Gene Symbol :

CDK2

Symbol Nomenclature :

CDK2

SYNONYM :

p33(CDK2)

Designation :

cdc2-related protein kinase|cell devision kinase 2|cell division protein kinase 2|p33 protein kinase

Full Nomenclature :

cyclin-dependent kinase 2

GENEID :

1017

HGNC :

1771

MIM :

116953

Vega :

OTTHUMG00000170575

Target GenBank:

M68520.1

Pathways

NCI :

ATR signaling pathway

NCI :

BARD1 signaling events

NCI :

E2F transcription factor network

NCI :

FOXM1 transcription factor network

NCI :

FoxO family signaling

NCI :

IL2-mediated signaling events

NCI :

mTOR signaling pathway

NCI :

p53 pathway

NCI :

p73 transcription factor network

NCI :

Regulation of nuclear SMAD2/3 signaling

NCI :

Regulation of retinoblastoma protein

NCI :

Signaling events mediated by PRL

Panther :

p53 pathway

Panther :

p53 pathway feedback loops 2

Reactome :

Activation of ATR in response to replication stress

Reactome :

Activation of the pre-replicative complex

Reactome :

APC/C-mediated degradation of cell cycle proteins

Reactome :

CDK-mediated phosphorylation and removal of Cdc6

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

Cellular responses to stress

Reactome :

Cellular Senescence

Reactome :

Cyclin A/B1 associated events during G2/M transition

Reactome :

Cyclin A:Cdk2-associated events at S phase entry

Reactome :

Cyclin E associated events during G1/S transition

Reactome :

DNA Damage/Telomere Stress Induced Senescence

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

Factors involved in megakaryocyte development and platelet production

Reactome :

G0 and Early G1

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

G1/S Transition

Reactome :

G2 Phase

Reactome :

G2/M Checkpoints

Reactome :

G2/M Transition

Reactome :

Hemostasis

Reactome :

M/G1 Transition

Reactome :

Meiosis

Reactome :

Meiotic recombination

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic G2-G2/M phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Orc1 removal from chromatin

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes

Reactome :

Regulation of APC/C activators between G1/S and early anaphase

Reactome :

Regulation of DNA replication

Reactome :

Regulation of mitotic cell cycle

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

SCF(Skp2)-mediated degradation of p27/p21

Reactome :

Senescence-Associated Secretory Phenotype (SASP)

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

WikiPathway :

Cell cycle

WikiPathway :

DNA damage response

WikiPathway :

DNA Replication

WikiPathway :

G1 to S cell cycle control

WikiPathway :

ID signaling pathway

WikiPathway :

Integrated Breast Cancer Pathway

WikiPathway :

Integrated Cancer pathway

WikiPathway :

miRNA regulation of DNA Damage Response

WikiPathway :

TSH signaling pathway

Protein

SMART domain :

TyrKc : Tyrosine kinase, catalytic domain

SMART domain :

S_TKc : Serine/Threonine protein kinases, catalytic domain

UniProt :

P24941

UniProt :

O75100

HPRD :

00310

Clone

Cloning Information :

272185

HIP Master Clone ID :

202548

Original Clone ID :

FLH272185.01L

Organism

Species Specific ID:

1017

Genome

Chromosome :

Map Location :

12q13

Ensembl :

ENSG00000123374

Reagents

Labome :

CDK2-antibody