Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
p9T-c
Source:
Center for Eukaryotic Structural Genomics
Description:
Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI).
Comments:
None
Publications:
PMID: 19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Authors:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
EvNO00306573
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
hygromycin
Host Type: mycobacteria Marker: hygromycin
Host Type: mycobacteria Marker: hygromycin
Vector Name:
p9T-c
Synonyms:
''
Sequencing Primer:
Forward:
PtetO
Reverse:
Unknown
Description:
Mycoplasma coexpression expression vector with the PtetO promoter; tetracycline-inducible expression of insert; hygromycin resistance in bacteria and mycobacteria; restriction enzyme cloning (PtetO gene: AsiSI, PmeI; second constitutively expressed gene: PacI, SbfI).
Comments:
Target gene controlled by PtetO promoter for expression is cloned between AsiSI and PmeI restriction sites. Then, the second constitutive expression cassette with another target gene originally cloned into the intermediate vector p4-Sb, p4-Hb or p4-Ib is transferred to this vector between PacI and SbfI restriction sites.
Size (bp):
8203
Parent Vector:
None
Properties:
multiple cloning site, with tag/fusion/marker
Author Name:
Brian Fox
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Yong Chang
David Mead
Vinay Dhodda
Phil Brumm
Center for Eukaryotic Structural Genomics
PSI
Publications:
PMID:
19760663
Title: One-plasmid tunable coexpression for mycobacterial protein-protein interaction studies
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
MCS
MCS
multiple cloning site
376
418
bacterial origin
ColE
ColE1 replication origin (on complementary strand)
6361
6894
mycobacterial origin
myc ori
mycobacterial origin of replication
1048
3522
promoter
PtetO
UV15 with two tet operators; tetracycline-inducible promoter
65
370
promoter
Psmyc
Psmyc promoter
3784
4190
repressor protein gene
TET
tetR(B) Tet Repressor class B
4217
4840
selectable marker
HygR
hygromycin resistance cassette (on complementary strand)
4947
5945
trxn termination sequence
rrnB
2 rrnBT2 transcriptional terminators; located from nucleotides 24-43 and 3834-3853
24
43
trxn termination sequence
T4g32
T4g32 transcription terminator
939
957
