Detailed Vector Information: pVP65K

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pVP65K
Source:
Center for Eukaryotic Structural Genomics
Description:
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
None
Publications:
PMID: 18988018
Title: Flexi vector cloning.
PMID: 25854603
Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors:
University of Wisconsin
Center for Eukaryotic Structural Genomics
EvNO00306668
Price: 
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AVAILABLE
No restriction
Special MTA: PSI,Promega

Recommended Growth Condition:

Distributed in bacterial strain:
BR610
T1 phage resistant DH5-alpha
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.