Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
None
Publications:
PMID:18988018 Title: Flexi vector cloning. PMID:25854603 Title: "Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies"
Authors:
University of Wisconsin
Center for Eukaryotic Structural Genomics
EvNO00306668
Price:
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AVAILABLE
No restriction
Special MTA:
PSI,Promega
Recommended Growth Condition:
Distributed in bacterial strain:
BR610
T1 phage resistant DH5-alpha
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Bacterial (E. coli) expression with N-terminal 8xHis, MBP tag; kanamycin resistance in bacteria; restriction enzyme cloning (SgfI, PmeI); Cloning into this vector will remove the BarCat cassette.
Comments:
Vector contains a lethal BarCat cassette that requires the strain BR610 for growth. Lethal cassette is removed during cloning.
Size (bp):
7174
Parent Vector:
None
Properties:
bacterial expression
Author Name:
University of Wisconsin
Center for Eukaryotic Structural Genomics
PSI