Phb (Rattus norvegicus) in pVP16

Explanation of Terms

Gene:
Gene Symbol:  Phb
Gene Name:  prohibitin
Original Clone ID:
2189.1.91_GO.91364
Keyword:
partial cds
Species:
Rattus norvegicus
Type:
cDNA
Vector Name:
pVP16
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
Parent TargetDB: GO.59424; His8-MBP fusion prtotein with TEV protease cleavage site is produced; after cleavage the protein obtained will be the translated CDS with the addition of SER at the N-term
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
RnCD00310291
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 750nts
Open reading frame: 28 to 777

ACAAGTTTGTACAAAAAAGCAGGCTCCGAAAACCTGTACTTCCAGTCCGATGCCGGACAC
AGAGCTGTCATCTTCGACCGATTCCGTGGCGTGCAGGACATCGTGGTAGGGGAAGGGACT
CACTTCCTCATCCCCTGGGTACAGAAGCCAATCATCTTTGACTGCCGCTCTCGACCACGT
AATGTGCCGGTCATCACCGGCAGCAAAGACTTGCAGAATGTCAACATCACACTACGTATC
CTCTTCCGGCCGGTGGCCAGCCAGCTTCCTCGTATCTACACCAGCATTGGCGAGGACTAT
GATGAGCGGGTGCTGCCATCTATCACCACAGAGATCCTCAAGTCGGTGGTGGCTCGATTC
GATGCTGGAGAATTGATTACCCAGCGAGAGCTGGTCTCCAGGCAGGTGAGTGATGACCTC
ACAGAGCGAGCAGCAACATTCGGGCTCATCCTGGATGACGTGTCCCTGACACATCTGACC
TTCGGGAAGGAGTTCACAGAGGCGGTGGAAGCCAAACAGGTGGCTCAGCAGGAAGCAGAG
AGAGCCAGATTTGTGGTGGAAAAGGCTGAGCAGCAGAAGAAGGCGGCCATCATCTCTGCT
GAGGGTGACTCCAAAGCGGCTGAGCTGATCGCCAACTCACTGGCCACCGCCGGGGATGGC
CTGATCGAGCTGCGAAAGCTGGAAGCTGCTGAGGACATTGCTTATCAGCTCTCCCGCTCT
CGGAACATCACCTACCTGCCAGCAGGGCAGTCCGTGCTCCTCCAGCTCCCCCAGTAATAG
GACCCAGCTTTCTTGTACAAAGTGGT

Protein sequence predicted based on nucleotide sequence

ENLYFQSDAGHRAVIFDRFRGVQDIVVGEGTHFLIPWVQKPIIFDCRSRPRNVPVITGSK
DLQNVNITLRILFRPVASQLPRIYTSIGEDYDERVLPSITTEILKSVVARFDAGELITQR
ELVSRQVSDDLTERAATFGLILDDVSLTHLTFGKEFTEAVEAKQVAQQEAERARFVVEKA
EQQKKAAIISAEGDSKAAELIANSLATAGDGLIELRKLEAAEDIAYQLSRSRNITYLPAG
QSVLLQLPQ*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.91364
Recommended expression in:
Not Applicable