hepA (A. baylyi ADP1 )
In pMCSG19B (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  hepA
Gene Name:  ATP-dependent helicase HepA
Original Clone ID:
APC89136.2_SG84_pMCSG19b
Keyword:
partial cds
Species:
Acinetobacter baylyi ADP1
Type:
cDNA
Vector Name:
pMCSG19B
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: 2008-05-12 yield: imacI-44.685 and imacII-38.4
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
AaCD00310571
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 573nts
Open reading frame: 51 to 623

CTTCTGGCGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCATTGAAGCAT
TGCAAATGCAGGCTCAGATGCATCGCAGTCCTTTACGTGGTCTGATTGGAGCACGTGTGG
GGTTGATCCCGCATCAGCTCTATATTGCACATGAAGTTGGTCAGCGCTTTGCACCGCGTG
TATTACTGGCCGATGAAGTTGGTTTGGGTAAAACCATTGAAGCTGGTCTAATCATCCATC
AACAGCTTAAAACAGGACGTGCTGAGCGTATCCTGATTTTGGTACCAGACTCATTACAGT
ATCAGTGGATGATTGAAATGCGCCGCCGTTTCAATCTGCAATTTTCTCTGTTTGATTTGA
CCCGTACCGCCTCAATCAAAGAGCATGATCCTGATTTAAATCCGTTCCTGACAGAACAAT
GTATCATTGCCAGTGTCGATTTGATGGTTGACCACGATGATTTACGTGAGCAGGCACTTG
AAGCTGGCTTTGATTTATTGGTGGTTGATGAAGCCCATCACTTGATGTGGAACGAAGAAG
AAGGTGGTAATGACCGCTATGATCTAGTTGAGGAACTGGCACAGCAAACGCCAGGTGTCT
TGCTTTTGACTGCGACACCAGAATAACATTGGAAGTGGATAACGGATCCGAATTCGAGCT
CCGTCGACAAGCTT

Protein sequence predicted based on nucleotide sequence

IEALQMQAQMHRSPLRGLIGARVGLIPHQLYIAHEVGQRFAPRVLLADEVGLGKTIEAGL
IIHQQLKTGRAERILILVPDSLQYQWMIEMRRRFNLQFSLFDLTRTASIKEHDPDLNPFL
TEQCIIASVDLMVDHDDLREQALEAGFDLLVVDEAHHLMWNEEEGGNDRYDLVEELAQQT
PGVLLLTATPE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC89136.2
Recommended expression in:
Not Applicable