CD2412 (C. difficile 630 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CD630_24120
Gene Name:  negative regulator of gluconeogenesis
Original Clone ID:
APC62623_SG154_pMCSG19
Keyword:
full length
Species:
Clostridioides difficile 630
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
CdCD00310638
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 636nts
Open reading frame: 43 to 678

GTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCGTGATTATTATTCAGCTT
AATGAAAGACAACTAAAAATTATAGATATAGTTAAGGAAAATGAACCAATAACGAGTGAA
AGTATTGCCTCCAGCTTGAATGTGACTCGTGCTACACTTAGATCTGATTTAGCTATATTA
ACAATGACAGGAATATTAGACGCAAGACCAAAAGTTGGATATTTTTATTCAGGAGTTAGT
GAAATAAATTTAATTGGTAAAAGTGTAAAAGAAAAAACAGTAGAGGATATAATGAGCATG
CCTGTACTAGCTAAGAAGGATGAAAGTATATATGATGTAATAGTCACTATGTTTTTATCT
GATGTAGGGAGTATAGTTATTATAGATGAGAATGAAGAGTTATGTGGAGTTGTTTCCAGA
AAAGACTTGCTAAAAGCAACTATAGGAGGTTCTGATATAAATAAAATGCCAATAGGTATG
ATAATGACAAGAACTCCAAATGTAGTCACTTTAACTAAAGGAGCAAGTGTTTTATTAGCT
TCAAGAAAAATAATAGAACATGAAGTAGATTCGATTCCGATTGTTGAATATAAGGGAGAA
GACAAAAATCATATGAGAGTTGTTGGGAGAATATCAAAAACCAACATAACTAAGCTATTC
TTAGAAATAGTTGACAATTAACATTGGAAGTGGATAACGGATCCGAATTCGAGCTCCGTC
GACAAGCTT

Protein sequence predicted based on nucleotide sequence

VIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLAILTMTGILDARPKVGY
FYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVTMFLSDVGSIVIIDENEEL
CGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKGASVLLASRKIIEHEVDSIPI
VEYKGEDKNHMRVVGRISKTNITKLFLEIVDN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC62623
Recommended expression in:
Not Applicable