CD1325 (C. difficile 630 )
In pMCSG19 (MBP and His-tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CD630_13250
Gene Name:  tRNA 2-selenouridine synthase
Original Clone ID:
APC62643.1_SG154_pMCSG19
Keyword:
partial cds
Species:
Clostridioides difficile 630
Type:
cDNA
Vector Name:
pMCSG19
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
purifi_date: 2008-11-12 yield: imacI-18 and imacII-22
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
CdCD00310651
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 417nts
Open reading frame: 51 to 467

CTTCTGGCGTGGATCTGGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTGTCAGTTA
TAAAAATAGAAAAAGCACTTAAGCTAGATAAAGTAATATTTGTAGATGTGAGAACTGAAG
GAGAATATGAAGAAGACCATATATTGAATGCAATCAATATGCCTCTTTTTAAAAATAATG
AACATAATGAAGTTGGAACAATTTATAAGATGCAGGGAAAACATGAAGCAATACAAAAAG
GGTTTGACTATGTATCGTATAAATTAAAGGATATATATTTACAAGCAGCAGAACTTGCAT
TAAATTATGATAATATTGTCATATATTGTGCAAGAGGCGGAATGAGAAGTGGAAGTATAG
TAAATTTATTATCATCATTAGGTGTAAATGTATATCAACTTGAAGGTGGATATAAGGCTT
ATAGAAATTTTGTTTTAGAGTATTTAAGGCATGTAATGGATACAAAATAACATTGGAAGT
GGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTT

Protein sequence predicted based on nucleotide sequence

LSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNEHNEVGTIYKMQGKHEA
IQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGSIVNLLSSLGVNVYQLEGG
YKAYRNFVLEYLRHVMDTK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in APC62643.1
Recommended expression in:
Not Applicable