CD3531 (C. difficile 630 )
In pMCSG9 (MBP and His tagged expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CD630_35310
Gene Name:  polymerase/histidinol phosphatase-like protein
Original Clone ID:
APC61517.1_SG139_pMCSG9
Keyword:
partial cds
Species:
Clostridioides difficile 630
Type:
cDNA
Vector Name:
pMCSG9
Format: 
FUSION
Source:
Midwest Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
Midwest Center for Structural Genomics
Argonne National Laboratory
CdCD00311473
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 780nts
Open reading frame: 51 to 830

CTGATTACGATATCCCAGGTACCGAGAACCTGTACTTCCAATCCAATGCCTTGCTAACAG
AGTTACATTGTCATACAAGTATATCAGATAATAATTTTACTACTGAAGAAATAATAAAAA
AAGCAGCAGAAAAGAATATAAATATCTTAGCAATAACAAATCATGACACATTATCTGGAT
TGGATGAAGCTCTTGTGCTTGGAGAGCATTATGGAATAACAATAATACCAGGTATAGAAA
TTTCAGCCTATGATTATAAAAATAGAAAAAGAGTTCATATTCTTGGATATAACATAGATT
TAAACAGTCAAGAAATAAAAAATTTGTGCAATCCAATGGTATGTGACAGACATAAAGCAT
CTGTTAAGATGGTAAATAAAATTATAGATTTAGGATACAAAATATCTCTGGAAGATGTAA
AGAAATATTCATCAAAGACTGGAATATTTAAACAACATATAATGAAAGCTCTTATAGATA
AAGGATATACAGATAATATATATTCAAATCTATATAAAGAATTGTTTCATAGAGGAAACG
GCAAGGTATATGTATCTTTAAAATATGTGGATTACAGAGATGCAATAAAAGCTATAAAAA
ATTCTGGTGGAATTTGTGTGTTAGCTCACCCGGGTCAAATGGATAATTTTAGCGCTATTG
AAGAGATGGTAAAAGTAGGATTAGATGGAATAGAAGTATATCATCCATCTCACAACAAAG
AACTAGAAAAAGAGTCGCTTTCTTATGCTAAGAAGTATAATCTAGTGGTCACAGGAGGTT
CAGATTACCACGGATTTTATTCTCAACATAATTATGAACTTGGAAGCAAGTAACATTGGA
AGTGGATAACGGATCCGAATTCGAGCTCCGTCGACAAGCTT

Protein sequence predicted based on nucleotide sequence

LLTELHCHTSISDNNFTTEEIIKKAAEKNINILAITNHDTLSGLDEALVLGEHYGITIIP
GIEISAYDYKNRKRVHILGYNIDLNSQEIKNLCNPMVCDRHKASVKMVNKIIDLGYKISL
EDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSNLYKELFHRGNGKVYVSLKYVDYRDAIK
AIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYHPSHNKELEKESLSYAKKYNLVV
TGGSDYHGFYSQHNYELGSK

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Tested_Not_Found
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in APC61517.1
Recommended expression in:
Not Applicable