MCA1834 (M. capsulatus str. Bath )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCA1834
Gene Name:  chloromuconate cycloisomerase
Original Clone ID:
9454d1BCt1p1
Keyword:
None
Species:
Methylococcus capsulatus str. Bath
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
McCD00311758
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1092nts
Open reading frame: 51 to 1142

CCCTCTGAATATTTTGTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGATCGCCG
ACATCCAGGTGCGGACCGAACATTTTCCGCTGACGCGTCCCTACCGCATCGCATTCCGCT
CGATCGAGGAAGTCGACAACCTCATCGTCGAAATCAGGACCGCCGACGGACTGCTCGGAC
TGGGCGCCGCCTCGCCCGAACGGCATGTCACCGGCGAAACCCTGGAAGCCTGCCACGCCG
CTTTGGATCATGATCGTCTCGGGTGGCTGATGGGCCGGGACATCCGGACCCTGCCGCGGC
TGTGCCGGGAACTGGCCGAACGGCTGCCTGCCGCGCCGGCCGCCCGCGCCGCTCTCGACA
TGGCGCTGCACGATCTGGTGGCCCAGTGTCTCGGCCTGCCCCTGGTCGAAATTTTGGGAC
GCGCCCACGACAGCTTGCCGACCTCGGTCACGATCGGCATCAAGCCGGTCGAAGAAACGC
TGGCCGAGGCGCGCGAACATCTGGCGCTCGGCTTCCGGGTGCTCAAGGTCAAGCTTTGCG
GCGACGAGGAGCAAGACTTCGAACGCCTGCGCCGGCTGCACGAAACGCTGGCCGGGCGGG
CCGTCGTGCGGGTCGATCCCAATCAGAGCTACGATCGCGACGGCCTGCTCCGTCTGGACC
GGCTGGGGCAGGAACTCGGCATCGAGTTCATCGAACAGCCGTTCCCGGCAGGGCGAACCG
ACTGGTTGCGGGCGCTCCCGAAAGCGATACGGCGCCGGATCGCCGCCGACGAATCCCTGC
TGGGCCCCGCCGATGCCTTCGCTTTGGCTGCACCGCCGGCCGCCTGCGGCATCTTCAACA
TCAAGCTCATGAAGTGCGGAGGGCTGGCCCCGGCGCGGCGTATCGCCACGATCGCCGAAA
CCGCCGGGATCGATCTGATGTGGGGCTGCATGGACGAGAGCCGCATCAGCATCGCCGCCG
CCCTGCACGCCGCCCTCGCCTGCCCGGCCACCCGCTACCTGGACCTGGACGGCAGCTTCG
ACCTGGCCCGCGACGTCGCCGAAGGCGGCTTCATCCTCGAGGATGGCCGGCTCCGGGTGA
CCGAACGGCCCGGCCTCGGACTCGTATACCCGGATGAAGGGCATCATCACCATCACCACT
GATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

KIADIQVRTEHFPLTRPYRIAFRSIEEVDNLIVEIRTADGLLGLGAASPERHVTGETLEA
CHAALDHDRLGWLMGRDIRTLPRLCRELAERLPAAPAARAALDMALHDLVAQCLGLPLVE
ILGRAHDSLPTSVTIGIKPVEETLAEAREHLALGFRVLKVKLCGDEEQDFERLRRLHETL
AGRAVVRVDPNQSYDRDGLLRLDRLGQELGIEFIEQPFPAGRTDWLRALPKAIRRRIAAD
ESLLGPADAFALAAPPAACGIFNIKLMKCGGLAPARRIATIAETAGIDLMWGCMDESRIS
IAAALHAALACPATRYLDLDGSFDLARDVAEGGFILEDGRLRVTERPGLGLVYPDEGHHH
HHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-9454d
Recommended expression in:
Not Applicable