bioB (H. influenzae )
In pSGX2 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  bioB
Gene Name:  biotin synthetase
Original Clone ID:
6107d3BNt7b1
Keyword:
None
Species:
Haemophilus influenzae
Type:
cDNA
Vector Name:
pSGX2
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"N-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
HiCD00311874
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 957nts
Open reading frame: 51 to 1007

CTTTAGAAGGAGATATACATATGGCTCATCATCACCATCACCACTCCCTTGAATATTGGT
CTGTTTGCAAAGTTGAGGCGTTATTTGAAACGCCATTTTTGGAATTAGTTTACCGAGCAA
CGCAAGTTCATCGTAAGCATTTTAATCCTCGCGCGATTCAGTTATCCACGTTAATGTCTA
TCAAAACGGGGGGATGCCCAGAAGATTGTAGTTATTGCCCTCAATCAGCCCGTTATCATA
CTGGCGTACAAAATCAGCAGTTATTAGATGTTGATGAGATCGTCGCTAAAGCAAAAATTG
CGAAAGCACGTGGTGCAGGGCGTTTCTGTATGGGGGCTGCTTGGCGAGGCCCTAAGCCAA
AAGATATTGAGAAAGTCACAGAAATTATTAAAGCCGTGAAATCACTTGGTTTAGAAACTT
GCGGTACCTTTGGTTTATTGCAAGACGGTATGGCGGAAGATTTAAAAGAAGCTGGATTAG
ATTATTACAATCATAATCTTGATACCGCACCAGAACATTATGCAGAAGTGATTGGTACTC
GCCGTTTTGATGATCGCCTTAGCACCTTAGGAAAAGTGCGCAAAGCTGGATTAAAAGTGT
GTTGTGGTGGCATTGTAGGGATGAATGAAACTCGCAAAGAACGGGCTGGATTAATCGCGA
GCCTTGCAAATCTTGATCCGCAACCTGAGTCAGTGCCGATTAATCAACTCGTTAAGGTTG
AAGGTACGCCGTTAGCCGATGCGGAAGAATTAGATTGGACGGAGTTTGTGCGAACGATTG
CTGTTGCACGTATTACTATGCCAAAAAGTTATGTTCGCCTTTCTGCAGGTCGCAGTGGTA
TGACTGAAGAAATGCAAGCGATGTGTTTTATGGCGGGTGCAAATTCTATTTTCTATGGCG
ATAAATTACTTGTTACAGATAATCCAGAAGAAGATGGCGATCAGTTATTGATGGCAAAAT
TAGATTTAGAGCCAGAAACTGCAGAAAATAAAAAGTTGGAAGGGTGATCCGAATTCGAGC
TCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

EYWSVCKVEALFETPFLELVYRATQVHRKHFNPRAIQLSTLMSIKTGGCPEDCSYCPQSA
RYHTGVQNQQLLDVDEIVAKAKIAKARGAGRFCMGAAWRGPKPKDIEKVTEIIKAVKSLG
LETCGTFGLLQDGMAEDLKEAGLDYYNHNLDTAPEHYAEVIGTRRFDDRLSTLGKVRKAG
LKVCCGGIVGMNETRKERAGLIASLANLDPQPESVPINQLVKVEGTPLADAEELDWTEFV
RTIAVARITMPKSYVRLSAGRSGMTEEMQAMCFMAGANSIFYGDKLLVTDNPEEDGDQLL
MAKLDLEPETAENKKLEG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-6107d
Recommended expression in:
Not Applicable