bioB (B. subtilis )
In pSGX2 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  bioB
Gene Name:  biotin synthase
Original Clone ID:
6107b2BNt1b1
Keyword:
None
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pSGX2
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"N-terminal 6-His, TOPO"
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BsCD00311876
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1002nts
Open reading frame: 51 to 1052

CTTTAGAAGGAGATATACATATGGCTCATCATCACCATCACCACTCCCTTAATCAATGGA
TGGAACTCGCAGACCGGGTGCTGGCTGGAGCAGAAGTGACTGACGAAGAGGCGCTTTCAA
TATTACATTGTCCTGATGAAGATATTTTGCTATTAATGCACGGGGCTTTTCACATCAGAA
AACACTTTTACGGAAAAAAAGTAAAGCTCAATATGATTATGAATGCGAAATCCGGGCTCT
GCCCGGAAAACTGCGGCTATTGTTCACAGTCTGCGATTTCGAAAGCGCCGATTGAGTCTT
ACCGGATGGTGAATAAGGAAACGCTGCTTGAAGGCGCGAAGCGGGCGCACGATCTGAATA
TCGGCACATATTGTATCGTGGCAAGCGGCAGAGGTCCGTCTAACAGAGAAGTGGATCAGG
TCGTAGATGCGGTTCAGGAAATTAAAGAGACGTATGGACTGAAGATTTGTGCATGTCTTG
GACTGTTGAAGCCAGAGCAGGCGAAGCGGCTCAAAGATGCAGGAGTAGACCGCTATAATC
ATAATTTGAATACGTCACAGAGAAACCATTCAAACATCACAACCTCACATACATACGATG
ACAGAGTCAATACGGTTGAAATCGCAAAAGAATCGGGGCTGTCTCCGTGTTCAGGCGCCA
TTATCGGGATGAAGGAGACGAAACAGGATGTCATTGACATCGCCAAAAGCTTGAAGGCTC
TTGACGCGGATTCCATTCCTGTGAATTTTTTGCATGCAATTGATGGCACGCCGTTAGAAG
GCGTCAACGAATTAAACCCGCTGTATTGTTTAAAAGTGCTGGCGCTGTTCCGTTTTATCA
ATCCATCAAAAGAAATTCGCATTTCCGGAGGAAGAGAGGTCAATCTCCGCACATTGCAGC
CATTAGGGCTTTACGCCGCAAACTCCATTTTTGTCGGAGACTACTTAACAACTGCCGGGC
AAGAGGAGACGGAGGATCATAAAATGCTGAGTGATTTAGGCTTTGAAGTTGAATCAGTCG
AAGAAATGAAGGCTAGTTTAAGTGAAGGGTGATCCGAATTCGAGCTCCGTCGACAAGCTT
GCGGCCGCACTCGAGCACCACCA

Protein sequence predicted based on nucleotide sequence

NQWMELADRVLAGAEVTDEEALSILHCPDEDILLLMHGAFHIRKHFYGKKVKLNMIMNAK
SGLCPENCGYCSQSAISKAPIESYRMVNKETLLEGAKRAHDLNIGTYCIVASGRGPSNRE
VDQVVDAVQEIKETYGLKICACLGLLKPEQAKRLKDAGVDRYNHNLNTSQRNHSNITTSH
TYDDRVNTVEIAKESGLSPCSGAIIGMKETKQDVIDIAKSLKALDADSIPVNFLHAIDGT
PLEGVNELNPLYCLKVLALFRFINPSKEIRISGGREVNLRTLQPLGLYAANSIFVGDYLT
TAGQEETEDHKMLSDLGFEVESVEEMKASLSEG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-6107b
Recommended expression in:
Not Applicable