Gene:
Gene Symbol:
dapE
Gene Name:
succinyl-diaminopimelate desuccinylase
Original Clone ID:
89b2TCt19b1
Keyword:
None
Species:
Neisseria meningitidis
Type:
cDNA
Vector Name:
pSB3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
"C-terminal 6-His, TOPO"
Mutation / Discrepancy:
Yes / No
Publications:
PMID: 16021622
Title: Structural analysis of a set of proteins resulting from a bacterial genomics project
Title: Structural analysis of a set of proteins resulting from a bacterial genomics project
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
NpCD00312065
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1173nts
Open reading frame: 51 to 1223
TCCCTCAGAAAATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTACCGAAACTC
AATCCCTAGAGCTTGCCAAAGAGCTGATTTCCCGACCATCCGTTACCCCTGACGACAGGG
ATTGCCAAAAACTCATGGCCGAACGCCTGCACAAAATCGGTTTTGCGGCTGAAGAAATGC
ATTTCGGCAACACCAAAAACATCTGGTTGCGACGCGGCACGAAAGCTCCCGTCGTCTGTT
TTGCAGGGCATACCGACGTTGTACCGACAGGTCCTGTCGAAAAATGGGATTCGCCCCCGT
TCGAACCGGCCGAGCGCGACGGAAGATTATACGGGCGCGGCGCGGCAGACATGAAAACCA
GCATTGCCTGTTTCGTTACCGCCTGCGAACGCTTCGTTGCCAAACATCCCAACCACCAAG
GCAGCATTGCGCTTCTGATTACTTCCGACGAAGAGGGCGACGCGTTGGACGGCACGACCA
AAGTCGTCGATGTATTGAAAGCGCGCGACGAGCTTATCGACTACTGCATCGTCGGCGAAC
CGACCGCCGTGGACAAATTGGGCGATATGATTAAAAACGGTCGGCGCGGCTCGCTGTCGG
GCAACCTGACCGTCAAAGGCAAGCAAGGCCATATTGCCTATCCGCATTTGGCAATCAATC
CCGTGCATACTTTTGCCCCGGCCTTGTTAGAGCTGACGCAGGAAGTCTGGGATGAAGGCA
ACGAATATTTCCCGCCAACCAGCTTTCAAATTTCCAATATCAACGGCGGCACAGGCGCGA
CCAACGTCATTCCGGGCGAGCTGAACGTCAAATTCAATTTCCGCTTCTCCACCGAGTCCA
CCGAAGCAGGGCTGAAACAACGCGTCCACGCCATTTTGGACAAACACGGCGTGCAATACG
ATTTGCAGTGGTCGTGTTCGGGGCAGCCCTTTCTGACCCAAGCGGGCAAACTGACCGACG
TGGCACGCGCAGCCATTGCCGAAACCTGCGGCATTGAGGCCGAATTGTCCACCACCGGCG
GCACTTCGGACGGACGCTTCATCAAAGCCATGGCGCAAGAACTTATCGAATTAGGCCCGT
CCAATGCGACCATCCACCAAATCAACGAAAACGTGCGGCTAAACGACATTCCGAAGCTGT
CTGCGGTGTATGAGGGGATATTGGTGCGCTTGTTGGCTGGAAATGCCGTCGAAGGGGGAT
CCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCG
CACTCGAGCACCAC
Protein sequence predicted based on nucleotide sequence
TETQSLELAKELISRPSVTPDDRDCQKLMAERLHKIGFAAEEMHFGNTKNIWLRRGTKAP VVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADMKTSIACFVTACERFVAKHP NHQGSIALLITSDEEGDALDGTTKVVDVLKARDELIDYCIVGEPTAVDKLGDMIKNGRRG SLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQEVWDEGNEYFPPTSFQISNINGG TGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAILDKHGVQYDLQWSCSGQPFLTQAGK LTDVARAAIAETCGIEAELSTTGGTSDGRFIKAMAQELIELGPSNATIHQINENVRLNDI PKLSAVYEGILVRLLAGNAVEGGSHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
L29M; L43M; D47N; I334M;
A372V;
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-89b
Recommended expression in:
Not Applicable
Vector Name:
pSB3
Synonyms:
pSB-3, pSB3_TC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
TOPO modified pSB3 vector -by Invitrogen. Addition of an extra G to the reverse primer which puts the vector-encoded C-terminal His-tag in-frame with the insert.
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
Initiation start (Met, Ser, Leu)
5072
5080
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4984
5003
repressor protein gene
LacI
LacI cds
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5092
5109
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5243
5289
